Optimization of growth, lipid and chlorophyll contents in Chlorella vulgaris under different conditions of nitrate, phosphate and photoperiod by central composite design (CCD)

	Optimization of growth, lipid and chlorophyll contents in Chlorella vulgaris under different conditions of nitrate, phosphate and photoperiod by central composite design (CCD)
مجله علمی شیلات ایران
Article 9, Volume 27, Issue 3, September and October 2018, Pages 85-95 PDF (1010.91 K)
Document Type: Research Paper
DOI: 10.22092/isfj.2018.117018
Authors
A. Vazirzadeh; H. Moghadaszadeh
Abstract
The effects of nitrate (750-2250 mg/L), phosphate (20-60 mg/L) and photoperiod (8-24 h) on the growth, chlorophyll content and lipid yield of Chlorella vulgaris were studied using central composite design (CCD) method. The second-order quadratic model was used to predict the effects of input variables on the output responses and the results were verified by F test, ANOVA and regression analyses. Results showed that maximum growth (255.88 ×10⁶)as well as highest chlorophyll content (15.34%) were observed at 16 h of light, 1500 mg/L of nitrate and 40 mg/L of phosphate concentration. Also, condition of 24 h of light, 750 mg/L nitrate and 20 mg/L phosphate concentrations resulted in maximum percent of lipid content (17.74%). The optimization of culture condition for simultaneous achievement of highest levels of growth, chlorophyll and lipid content were investigated.
Keywords
Environmental and nutritional conditions; Chlorella vulgaris; biochemical components

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