Effective factors on micropropagation of medicinal plant of Satureja khuzistanica | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| Article 7, Volume 26, Issue 1 - Serial Number 51, June 2018, Pages 53-62 PDF (642.88 K) | ||
| DOI: 10.22092/ijrfpbgr.2018.116656 | ||
| Authors | ||
| L. Mirjani1; A. Salimi* 2; M. Matinizadeh3; K. Razavi4; M. Shahbazi5 | ||
| 1Ph.D. student, College of Biological Sciences, Kharazmi University, Tehran, I.R. Iran. | ||
| 2Corresponding author, Assoc. Prof., College of Biological Sciences, Kharazmi University, Tehran, I.R. Iran, | ||
| 3Assoc. Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran. | ||
| 4Accis. Prof., National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, I.R.Iran. | ||
| 5Accis. Prof., Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, I.R. Iran. | ||
| Abstract | ||
| Satureja khuzistanica Jamzad is an endemic species of Iran. The species is an endangered and valuable medicinal plant. In order to propagate through micropropagation, lateral buds were collected from Lorestan province, region of Pol of Dokhtar. The best treatment of sterilization of the explants was 0.1% HgCl2 for 6 minutes. The best medium for shoot regeneration was MS with BA, 2iP and IBA 0.3, 0.3 and 1 mg/l, respectively. Applying 12 different treatments, the mentioned medium was better than other treatments in term of the coefficient of elongation, leaf elongation and leaf greenness. The best medium for root regeneration was MS with 1 mg/l IBA and 0.1 mg/l NAA. It initiated the highest number of primary and secondary roots. Various combinations of different soils were evaluated for survival during the acclimatization, and sand: soil: peat: perlite at a ratio of 1: 1: 1: 1 showed the highest percentage of survival and growth. The best propagation method of the species was developed and the propagated plants could be transplanted into the habitat of the species. | ||
| Keywords | ||
| In vitro regeneration; Micropropagation; Plant growth regulator and Satureja khuzistanica | ||
| References | ||
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