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تأثیر ازتوباکتر بر شاخص‌های رشدی، عملکرد و میزان اسانس دو توده محلی زیره سبز (Cuminum cyminum L.) در شرایط شوری | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 8، دوره 34، شماره 2 - شماره پیاپی 88، خرداد و تیر 1397، صفحه 261-271 اصل مقاله (266.95 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2018.120473.2244 | ||
| نویسندگان | ||
| هنگامه وثوقی تبار1؛ سید علی حسینی تفرشی2؛ حمید دهقان زاده* 3 | ||
| 1کارشناس ارشد، گروه علوم کشاورزی، دانشگاه پیام نور، تهران، ایران | ||
| 2استادیار، گروه بیوتکنولوژی، دانشکده شیمی، دانشگاه کاشان، کاشان، ایران | ||
| 3استادیار، گروه علوم کشاورزی، دانشگاه پیام نور، تهران، ایران | ||
| چکیده | ||
| به‌منظور بررسی تأثیر ازتوباکتر بر شاخص‌های رشدی، عملکرد دانه و میزان اسانس دو توده محلی زیره سبز (Cuminum cyminum L.) در شرایط شوری، آزمایشی به‌صورت فاکتوریل در قالب بلوک‌های کامل تصادفی و در سه تکرار در سال 1394 در دانشگاه کاشان به‌صورت گلدانی انجام شد. تیمارهای آزمایش شامل دو توده محلی زیره سبز اردستان و مشهد اردهال، چهار سطح شوری (صفر، 100، 150 و 200 میلی‌مولار کلرید سدیم) و دو سطح باکتری ازتوباکتر (تلقیح و عدم تلقیح بذر) بودند. نتایج نشان داد که شوری و تلقیح بذر با باکتری تأثیر بسیار معنی‌داری بر درصد و سرعت جوانه‌زنی، طول ریشه‌چه و ارتفاع ساقه‌چه داشت. افزایش غلظت کلرید سدیم تا سطح 200 میلی‌مولار منجر به کاهش معنی‌دار درصد جوانه‌زنی و سرعت جوانه‌زنی، طول ریشه‌چه، ارتفاع ساقه‌چه، عملکرد دانه و عملکرد اسانس شد. همچنین نتایج نشان داد در هر دو توده و در تمام سطوح شوری، تلقیح با باکتری در مقایسه با شاهد، منجر به افزایش معنی‌دار کلروفیل a، کلروفیل b، میزان آنزیم‌های کاتالاز، آسکوربات پراکسیداز، عملکرد دانه و عملکرد اسانس گردید. توده محلی اردستان کلروفیل a و b، آنزیم‌های کاتالاز و آسکوربات پراکسیداز درصد اسانس بیشتری در مقایسه با توده محلی مشهد اردهال داشت و شرایط شوری را بهتر تحمل نمود. در شرایط شوری تلقیح بذرها با باکتری ازتوباکتر به‌دلیل تأثیرات مثبت، توانست اثرات منفی تنش را کاهش داده و سرعت جوانه‌زنی، عملکرد دانه و عملکرد اسانس را نسبت به نمونه‌های شاهد بهبود ببخشد. بنابراین می‌توان در شرایط مشابه با این آزمایش، توده محلی اردستان را کشت نموده و برای کاهش اثرات شوری از تلقیح بذر با ازتوباکتر استفاده و عملکرد دانه و اسانس بالاتری بدست آورد. | ||
| کلیدواژه‌ها | ||
| ازتوباکتر؛ آنتی‌اکسیدان؛ تنش شوری؛ زیره (Cuminum cyminum L.)؛ سرعت جوانه‌زنی | ||
| عنوان مقاله [English] | ||
| Effect of azetobacter on growth indices, yield and essence content of two cumin (Cuminum cyminum L.) landraces under salinity conditions | ||
| نویسندگان [English] | ||
| H. Vosoughi Tabar1؛ S.A. Hosseini Tafreshi2؛ H. Dehghanzadeh3 | ||
| 1Department of Agricultural Sciences, Payame Noor University (PNU), Tehran, Ira | ||
| 2Department of Biotechnology, Faculty of Chemistry, University of Kashan, Kashan, Iran | ||
| 3Department of Agricultural Sciences, Payame Noor University (PNU), Tehran, Iran | ||
| چکیده [English] | ||
| In order to investigate the effects of Azotobacter on growth indices, yield and essential oil content of two cumin (Cuminum cyminum L.) landraces under salinity conditions, an experiment was carried out in a factorial design arranged as randomized complete block design with three replications in 2015 at the Kashan University. The treatments included two cumin landraces (Ardestān and Mashhad Ardehal), four salinity levels (control, 100, 150 and 200 mili molar Nacl) and two levels of azotobacter (control and seed inoculation). Results showed that salinity and inoculation with bacteria had a very significant effect on germination rate and percentage, radicle length and plumule height. Increased concentrations up to 200 Mm sodium chloride resulted in a significant reduction in germination percentage and germination rate, radicle length, plumule height, grain yield and essential oil yield. According to the results, in both landraces and at all salinity levels, inoculation with bacteria, resulted in a significant increase in chlorophyll a, b, catalase, ascorbate peroxidase, grain yield and essential oil yield compared to the control. The Ardestān landrace had the highest chlorophyll a and b, catalase, ascorbate peroxidase and essential oil content and tolerated salinity better compared to the Ardehal Mashhad landrace. In salinity conditions, inoculation of seeds with Azotobacter could reduce the negative effects of stress and improve the germination rate, seed yield and essential oil yield. The results of this experiment indicated that Ardestān landrace could be cultivated and used to reduce the effects of salinity due to the inoculation with Azotobacter, and higher grain yield and essential oil yield could be obtained under conditions similar to this experiment. | ||
| کلیدواژه‌ها [English] | ||
| Azotobacter, Antioxidant, Salinity stress, cumin (Cuminum cyminum L.), germination speed | ||
| مراجع | ||
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