Naraghi, T. (2003). In Vitro Propagation of Taxus Baccata Through Shoot Tip Culture. , 11(3), 309-325. doi: 10.22092/ijrfpbgr.2003.116091
T. S. Naraghi. "In Vitro Propagation of Taxus Baccata Through Shoot Tip Culture". , 11, 3, 2003, 309-325. doi: 10.22092/ijrfpbgr.2003.116091
Naraghi, T. (2003). 'In Vitro Propagation of Taxus Baccata Through Shoot Tip Culture', , 11(3), pp. 309-325. doi: 10.22092/ijrfpbgr.2003.116091
Naraghi, T. In Vitro Propagation of Taxus Baccata Through Shoot Tip Culture. , 2003; 11(3): 309-325. doi: 10.22092/ijrfpbgr.2003.116091

In Vitro Propagation of Taxus Baccata Through Shoot Tip Culture

تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران
Article 3, Volume 11, Issue 3 - Serial Number 11, March 2003, Pages 309-325
DOI: 10.22092/ijrfpbgr.2003.116091
Author
T. S. Naraghi*
Research Institute of Forests And Rangelands, Department of Plant Genetics and Physiology, Tehran, Iran
Abstract
In order to investigate in vitro culture of Taxus baccata, apical buds were cut in autumn from selected mature trees at Astara, Asalem forest in Gillan and Aliabadkatol in Golestan province, Iran. Application of 0.1% mercuric chloride solution for 10 minutes was the most effective way for the surface sterilization of the buds. Then the buds were cultured on MS (N/2), B5 and MCM medium plus BA (0.5- 1 mg/L) + IBA 0.1 mg/L. The best treatment for shoot proliferation and elongation was MS (N/2) medium containing 0.5 mg/L BA+ 0.1 mg/L IBA.
The shoots were treated as cuttings by dipping the basal ends in 50 mg/L IBA solution for 24 hours, then they were placed in an auxin- free medium plus 1% activated charcoal, for stimulating rooted shoot propagation. Rooted plantlets after acclimation were transferred to mixed soil with equal parts of (vermiculate, sand and peat) under greenhouse conditions.
Keywords
Taxus Baccata (Yew); In vitro culture; Apical Bud; Shoot- Tip CULTURE
References
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