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بررسی اثر گیاه دارویی خار مریم بر اندازه قطر هسته و شکست DNA اسپرم تیمار شده با کلرید کادمیوم در قوچ نژاد فراهانی | ||
| علوم دامی | ||
| مقاله 18، دوره 30، شماره 117، اسفند 1396، صفحه 215-226 اصل مقاله (1.06 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2017.110573.1450 | ||
| نویسندگان | ||
| عاطفه خاوری1؛ مهدی خدایی مطلق* 2؛ حمیدرضا مومنی3 | ||
| 1دانشآموخته کارشناسی ارشد گروه زیست شناسی دانشکده علوم پایه دانشگاه اراک. | ||
| 2دانشیارگروه علوم دامی دانشکده کشاورزی و منابع طبیعی دانشگاه اراک. | ||
| 3دانشیار گروه زیست شناسی دانشکده علوم پایه دانشگاه اراک. | ||
| چکیده | ||
| اسپرم مورد استفاده در مطالعه حاضر از اپیدیدیم بیضههای قوچهای فراهانی کشتار شده جمعآوری و به چهار گروه تقسیم شد. در هر نمونه، 106× 5 اسپرم وجود داشت. تیمارهای آزمایشی شامل اسپرمهای لحظه صفر، اسپرمهای لحظه 180 دقیقه (شاهد)، اسپرمهای تیمار شده با کلرید کادمیوم (10 میکرومولار) به مدت 180 دقیقه و اسپرمهای تیمار شده با سیلیمارین (5 میکرومولار) همراه با کلرید کادمیوم (10 میکرومولار) به مدت 180 دقیقه بودند. برای بررسی جنبههای مورفولوژیکی آپوپتوز در هسته اسپرم از رنگآمیزیDiff-Quick استفاده گردید. همچنین برای برآورد شکست DNA از رنگآمیزی اکریدین اورنژ و تست SCD استفاده شد. آنالیز دادهها از طریق روش تجزیه واریانس یک طرفه صورت گرفت. مقایسه میانگین تیمارها با استفاده از آزمون توکی در سطح خطای 05/0 انجام شد. در این پژوهش، قطر هسته اسپرم در گروه تیمار شده با کلرید کادمیوم نسبت به گروه شاهد به طور معنیداری (05/0P<) کاهش یافت. استفاده از سیلیمارین همراه با کلرید کادمیوم توانست کاهش قطر هسته اسپرم را نسبت به گروه تیمار شده با کلرید کادمیوم به طور معنیداری جبران کند. همچنین نتایج نشان دادند که درصد شکست DNA در گروه تیمار شده با کلرید کادمیوم در مقایسه با گروه شاهد (001/0>P) و گروه تیمار شده با سیلیمارین همره با کلرید کادمیوم (01/0>P) به طور معنیداری افزایش یافت | ||
| کلیدواژهها | ||
| "کادمیوم کلراید"؛ " سیلیمارین"؛ " اسپرم"؛ " آپوپتوزیس"؛ " قوچ فراهانی" | ||
| عنوان مقاله [English] | ||
| Effects of medicinal plant Silybum marianum on core diameter and DNA fragmentation in the sperm of rams Farahani treated with cadmium | ||
| نویسندگان [English] | ||
| Atefeh Khavari1؛ Mahdi Khodaei2؛ HamidReza Momeni3 | ||
| 1Department of Biology | ||
| 2Department of Animal Science Faculty of Agriculture Arak university | ||
| 3Department of biology faculty of Science Arak university | ||
| چکیده [English] | ||
| The sperm used in the current study was collected from the testis epidydim of slaughtered Farahani's ram and divided to four groups. There are 106 sperms in each sample. The experimental treatments included sperm at 0 hour, sperm at 180 minutes (control), sperm treated with cadmium chloride (10μM) for 180 minutes and sperm treated with silymarin (5 μM) and cadmium chloride (10 μM) for 180 minutes. Diff-Quick staining was used to investigate the apoptosis morphological features in sperm nucleus. Also, acridine orange staining and sperm chromatin dispersion (SCD) test were used for estimation of sperm DNA fragmentation. Data were analyzed using one way ANOVA. Comparison of treatment means was done by Tukey's test at 0.05 confidence level.In this research, the sperm nucleus diameter was significantly (P< 0.05) decreased in cadmium chloride-treated group when compared with control. The utilization of silymarin with cadmium chloride could compensate significantly the reduction in sperm nucleus diameter in cadmium chloride- treated group. The results also showed that the DNA fragmentation percentage was significantly increased in cadmium chloride- treated group in comparison to control (P<0.001) and silymarin with cadmium chloride-treated group (P< 0.01). Silymarin as a potent antioxidant can inhibit the detrimental effect of cadmium chloride on ram sperm nucleus diameter and DNA integrity. | ||
| کلیدواژهها [English] | ||
| "cadmium chloride", " silymarin", "sperm", " apoptosis", "Farahani´s ram" | ||
| مراجع | ||
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