Micropropagation of wild servis tree (Sorbus torminalis (L.) Crantz) 1- Shoot Proliferation | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| Article 7, Volume 6, Issue 1 - Serial Number 6, March 2001, Pages 115-129 PDF (477.76 K) | ||
| DOI: 10.22092/ijrfpbgr.2001.115874 | ||
| Author | ||
| M. Nasiri* | ||
| Scientific member of Research lnstitute of Forests and Rangelands | ||
| Abstract | ||
| Wild servis tree (Sorbus tormhnalis) is an important forest species for wood production in Iran. This experiment was conducted for shoot proliferation in this species. Apical and lateral buds from elite trees were collected in northern forest (Loveh, Farim and Pardcola) in different seasons (spring, autumn and winter). Sterilized explants were then cultured on basal MS medium. The results showed that the best treatments for explants sterilizing were: -1% (w/v) Benomyl for 30 minutes - 70% Ethanol for 5 seconds -0.1% Mercuric chloride with 1-2 drops of liquid soap for 1 minute The results indicated that the best season for taking explant from mother plants was late autumn. The suitable media for explants establishment and shoot proliferation were basal MS suplemented with 0.5 ppm BAP and 1/2N basal medium with 1 ppm BAP, respectively. Using GA3 was not effective for stopping rosette growth and increasing internode length. | ||
| Keywords | ||
| Sorbus torminalis; Micropropagation; Wild Servis; Tree | ||
| References | ||
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