| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شمارهها | 3,196 |
| تعداد مقالات | 35,423 |
| تعداد مشاهده مقاله | 52,243,825 |
| تعداد دریافت فایل اصل مقاله | 91,777,238 |
Experimental Infection of Turkeys with A Virulent Newcastle Disease Virus Isolated from Broiler Chickens | ||
| Archives of Razi Institute | ||
| مقاله 6، دوره 74، شماره 1، خرداد 2019، صفحه 51-57 اصل مقاله (1.06 M) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2017.115524.1149 | ||
| نویسندگان | ||
| R. A. Jafari* 1؛ Z. Boroomand1؛ Annahita Rezaie2؛ M. Mayahi1؛ A. Nejati Saravi3 | ||
| 1Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| 2Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran | ||
| 3Postgraduate Student of Avian Medicine, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| چکیده | ||
| Newcastle disease (ND) is a highly contagious infection of many avian species, mainly chickens and turkeys, with a devastating impact on worldwide poultry production. The ND accounts for heavy losses in Iranian poultry flocks. There are some reports regarding the epidemiology of this infection in Iran. This study was performed to investigate the infection of turkeys with a Newcastle disease virus (NDV) isolated from a broiler chicken flock in southwestern Iran during 2013. For the purpose of the study, 70 day-old Wishard bronze poults were allocated into two groups of control (n=25) and infected (n=45). At 32 days of age, each bird in the infected group was inoculated with 0.1 mL (50 μL per eye) of NDV-infected allantoic fluid through an ocular route and received 105 EID50 of viral inoculum. On the other hand, the birds in the control group were inoculated with phosphate buffered saline by the same route. Swab samples were taken from both groups at different time points, namely from 1 to 21 days postinoculation, and verified for NDV infection by using reverse transcription-polymerase chain reaction (RT-PCR). Both groups were also examined serologically by haemagglutination inhibition test. Clinically, the infected turkeys exhibited anorexia, severe depression, sitting on the hock joint, white to greenish (sometimes bloody) diarrhea, neurological disorders, and mild respiratory problems. Out of 45 inoculated birds, 9 (20%) cases died. Based on RT-PCR, virus shedding was observed in the challenged birds 3-8 days postinoculation. The NDV was detected more in tracheal swabs (50%) than in cloacal swabs (12.5%). The infected birds showed a high seroconversion. Therefore, the NDV circulating in Iranian chicken flocks has the potential to cause a serious illness in commercial turkeys. The vaccination of turkeys, as well as biosecurity, should be considered carefully to prevent the ND outbreaks in the future. | ||
| کلیدواژهها | ||
| Newcastle disease؛ Turkeys؛ Immune response؛ pathogenicity؛ RT-PCR | ||
| مراجع | ||
|
Ahmadi, E., Pourbakhsh, S.A., Ahmadi, M., Mardani, K., Talebi, A., 2016. Phylogenetic characterization of virulent Newcastle disease viruses isolated during outbreaks in northwestern Iran in 2010. Arch Virol 161, 3151-3160.
Alexander, D.J., Manvell, R.J., Banks, J., Collins, M.S., Parsons, G., Cox, B., et al., 1999. Experimental assessment of the pathogenicity of the Newcastle disease viruses from outbreaks in Great Britain in 1997 for chickens and turkeys, and the protection afforded by vaccination. Avian Pathol 28, 501-511.
Alexander, D.J., Morris, H.T., Pollitt, W.J., Sharpe, C.E., Eckford, R.L., Sainsbury, R.M., et al., 1998. Newcastle disease outbreaks in domestic fowl and turkeys in Great Britain during 1997. Vet Record 143. 212-209.
Boroomand, Z., Jafari, R.A., Mayahi, M., 2016. Molecular characterization and phylogenetic study of the fusion genes of Newcastle disease virus from the recent outbreaks in Ahvaz, Iran. Virus Dis 27, 102-105.
Capua, I., Dalla, P.M., Mutinelli, F., Marangon, S., Terregino, C., 2002. Newcastle disease outbreaks in Italy during 2000. Vet Record 150, 565-568.
Diel, D.G., Susta, L., Cardenas Garcia, S., Killian, M.L., Brown, C.C., Miller, P.J., et al., 2012. Complete genome and clinicopathological characterization of a virulent Newcastle disease virus isolate from South America. J Clin Microbiol 50, 378-387.
Gale, C., Mc, C.M., Sanger, V.L., 1961. Newcastle disease in turkeys. J Am Vet Med Assoc 139, 462-465.
Ghiamirad, M., Pourbakhsh, A., Keyvanfar, H., Momayaz, R., Charkhkar, S., Ashtari, A., 2010. Isolation and characterization of Newcastle disease virus from ostriches in Iran. Afr J Microbiol Res 4, 2492-2497.
Gowthaman, V., Singh, S.D., Barathidasan, R., Ayanur, A., Dhama, K., 2013. Natural Outbreak of Newcastle Disease in Turkeys and Japanese Quails Housed Along With Chicken in a Multi–Species Poultry Farm in Northern India. Advanc Anim Vet Sci 1, 17-20.
Graham, D.A., Connor, T.J., McCullough, S.J., McKillop, E.R., Alexander, D.J., Manvell, R.J., et al., 1996. Isolation and characterisation of an avian paramyxovirus type 1 from turkeys in Northern Ireland. Vet Record 138, 416-417
Haque, M., Hossain, M., Islam, M., Zinnah, M., Khan, M., Islam, M., 2010. Isolation and detection of Newcastle disease virus from field outbreaks in broiler and layer chickens by reverse transcription-polymerase chain reaction. J Vet Med 8, 87-92.
Heckert, R.A., Collins, M.S., Manvell, R.J., Strong, I., Pearson, J.E., Alexander, D.J., 1996. Comparison of Newcastle disease viruses isolated from cormorants in Canada and the USA in 1975, 1990 and 1992. Can J Vet Res 60, 50-54.
Kaleta, E.F., Baldauf, C., 1988. Newcastle disease in free- living and pet birds. Newcastle disease, Springer, pp. 197-246.
Madadgar, O., Karimi, V., Nazaktabar, A., Kazemimanesh, M., Ghafari, M.M., Azimi Dezfouli, S.M., et al., 2013. A study of Newcastle disease virus obtained from exotic caged birds in Tehran between 2009 and 2010. Avian pathology: journal of the W.V.P.A 42, 27-31.
Manual, O.T., 2008. World organisation for animal health manual of diagnostic tests and vaccines for terrestrial animals (mammals, birds and bees). Volume 2, 2008.
Mehrabanpour, M.J., Khoobyar, S., Rahimian, A., Nazari, M.B., Keshtkar, M.R., 2014. Phylogenetic characterization of the fusion genes of the Newcastle disease viruses isolated in Fars province poultry farms during 2009-2011. Veterinary research forum: an international quarterly journal 5, 187-191.
Miller, P.J., Koch, G., 2013. Newcastle disease. Diseases of poultry 13, 89-138.
Piacenti, A.M., King, D.J., Seal, B.S., Zhang, J., Brown, C.C., 2006. Pathogenesis of Newcastle disease in commercial and specific pathogen-free turkeys experimentally infected with isolates of different virulence. Veterinary pathology 43, 168-178.
Villegas, P., 2008. Titration of biological suspensions, p 217–221. A laboratory manual for the isolation, identification, and characterization of avian pathogens. American Association of Avian Pathologists, Kennett Square, PA.
Wakamatsu, N., King, D.J., Kapczynski, D.R., Seal, B.S., Brown, C.C., 2006. Experimental pathogenesis for chickens, turkeys, and pigeons of exotic Newcastle disease virus from an outbreak in California during 2002-2003. Veterinary pathology 43, 925-933. | ||
|
آمار تعداد مشاهده مقاله: 2,790 تعداد دریافت فایل اصل مقاله: 3,397 |
||