Sobhani, M., Lotfi, M., Seyfi Abad Shapouri, M., Ghiami Rad, M. (2018). Evaluation of MDBK, BT, CEF and Vero cell lines sensitivity for replication of bovine parainfluenza virus type 3 (bPI3V) in comparison with RBK cell line. , 31(1), 48-57. doi: 10.22092/vj.2017.114572.1336
M. Sobhani; M. Lotfi; M.R. Seyfi Abad Shapouri; Mehdi Ghiami Rad. "Evaluation of MDBK, BT, CEF and Vero cell lines sensitivity for replication of bovine parainfluenza virus type 3 (bPI3V) in comparison with RBK cell line". , 31, 1, 2018, 48-57. doi: 10.22092/vj.2017.114572.1336
Sobhani, M., Lotfi, M., Seyfi Abad Shapouri, M., Ghiami Rad, M. (2018). 'Evaluation of MDBK, BT, CEF and Vero cell lines sensitivity for replication of bovine parainfluenza virus type 3 (bPI3V) in comparison with RBK cell line', , 31(1), pp. 48-57. doi: 10.22092/vj.2017.114572.1336
Sobhani, M., Lotfi, M., Seyfi Abad Shapouri, M., Ghiami Rad, M. Evaluation of MDBK, BT, CEF and Vero cell lines sensitivity for replication of bovine parainfluenza virus type 3 (bPI3V) in comparison with RBK cell line. , 2018; 31(1): 48-57. doi: 10.22092/vj.2017.114572.1336

Evaluation of MDBK, BT, CEF and Vero cell lines sensitivity for replication of bovine parainfluenza virus type 3 (bPI3V) in comparison with RBK cell line

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 6, Volume 31, Issue 1 - Serial Number 118, March 2018, Pages 48-57    PDF (3.69 M)
Document Type: Full Research Paper
DOI: 10.22092/vj.2017.114572.1336
Authors
M. Sobhani* 1; M. Lotfi2; M.R. Seyfi Abad Shapouri3; Mehdi Ghiami Rad4
1Department of Biology, Higher Education Institute of Rabe–Rashid, Tabriz, Iran.
2Razi Vaccine and Serum Research Institute, AgriculturalResearch, Education and Extension Organization (AREEO), Karaj, Iran.
3Department of Pathobiology, Faculty of Veterinary Medicine, ShahidChamran University, Ahvaz, Iran.
4Microbiology Department, Faculty of Basic science, Islamic Azad University Ahar branch, Ahar, Iran.
Abstract
Bovine parainfluenza virus type 3 (bPI3V), as one of the most common viral agents causing bovine respiratory disease complex, belongs to the genus Respirovirus. The aim of this study was to compare the sensitivity of RBK, MDBK, BT, Vero cell lines and CEF primary cell for proliferation of bPI3V. For this purpose, first the best MOI for the selected bPI3V strain was determined on RBK cell line, and then used for sensitivity assay of each above cell lines. Tests repeated three times in the same condition and data were analyzed with independent samples t-test also as a completely randomized design with LSD method (SAS 9.4). According to the results of the t-test, there is no significant difference between the growth of the bPI3V on the RBK with MDBK and BT cell lines, whereas the RBK cell line showed a statistically significant difference with respect to Vero and CEF cell lines separately (P-value 0.01). No significant differences were seen between the RBK, MDBK, and BT cell lines as well as between Vero and CEF cells based on LSD test results, but RBK, MDBK and BT in comparison with Vero and CEF are significantly different with 99% confidence. It can be concluded that the RBK cell line, due to its higher sensitivity to bPI3V, as well as easier and faster cell culturing, is the superior to the other cell lines in this study.
Keywords
Cell sensitivity; Bovine parainfluenza virus type 3; RBK; MDBK; BT; Vero; CEF
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