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تغییرات شاخص های فیزیولوژیک و نمایه متابولیتی مرزه خوزستانی (Satureja khuzistanica) در پاسخ به تنش خشکی | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 5، دوره 25، شماره 2 - شماره پیاپی 50، مهر 1396، صفحه 232-246 اصل مقاله (280.47 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2017.113313 | ||
| نویسندگان | ||
| آناهیتا شریعت1؛ قاسم کریم زاده* 2؛ محمد حسن عصاره3؛ جواد هادیان4 | ||
| 1دانش آموخته دکتری گروه اصلاح نباتات، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران | ||
| 2دانشیار، گروه اصلاح نباتات، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران | ||
| 3استاد، مؤسسه تحقیقات جنگلها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران | ||
| 4دانشیار، گروه مهندسی کشاورزی، پژوهشکده گیاهان و مواد اولیه دارویی، دانشگاه شهید بهشتی، تهران | ||
| چکیده | ||
| مرزه خوزستانی یکی از نه گونه انحصاری جنس مرزه در ایران محسوب میشود که بهدلیل داشتن ترکیب فنلی کارواکرول در اسانس و اسیدهای فنلی رزمارینیک و کافئیک در عصاره، دارای اثرات ضد میکروبی قوی میباشد که در درمان تعدادی از بیماریها مؤثر است. در این تحقیق، تنش خشکی از طریق روش قطع آبیاری در مرحله گلدهی بر یک کلن از مرزه خوزستانی اعمال شد. نمونهبرداری در فواصل سه روز و در پنج مرحله انجام شد و تعدادی از شاخصهای فیزیولوژیک اندازه گیری شد. بهمنظور استخراج اسانس و تعیین بازده آن از دستگاه کلونجر، برای تعیین درصد ترکیبات موجود در اسانس از دستگاه های GC و GC/MS و برای تعیین مقدار ترکیبات موجود در عصاره الکلی از دستگاه HPTLC استفاده شد. نمایه متابولیتی تهیه شده بر حسب الگوی زمان بیانگر روند افزایشی -کاهشی برای ترکیبات فنلی و ترپنهایی مانند تیمول، گاماترپینن، پاراسیمن، کافئیک اسید و رزمارینیک اسید بود. همچنین، تنش خشکی منجر به افزایش معنیدار بازده اسانس، قندهای محلول و پرولین و نیز کاهش معنیدار شاخصهای توان آبی برگ، محتوای نسبی آب برگ و رنگیزههای گیاهی شد. نمایه متابولیتی مرزه خوزستانی بیانگر راهبردهای بکار گرفته شده این گیاه در ایجاد فنوتیپهای بیوشیمیایی مختلف میباشد. همچنین از نتایج کاربردی این تحقیق میتوان به کاربرد اثربخش تنش خشکی قبل از برداشت محصول اشاره کرد که منجر به افزایش کیفی محصول میشود. این تحقیق بیانگر اثر تنش خشکی بر خصوصیات فیزیولوژیک و متابولیسم ثانویه میباشد تا در آینده با استفاده از مهندسی متابولیک این گونه با ارزش بتوان تولید ترکیبات مهمی مانند کارواکرول، رزمارینیک اسید و کافئیک اسید را افزایش داد. | ||
| کلیدواژهها | ||
| مرزه؛ تنش خشکی؛ متابولیتهای ثانویه؛ بازده اسانس | ||
| عنوان مقاله [English] | ||
| Variations of physiological indices and metabolite profiling in Satureja khuzistanica in response to drought stress | ||
| نویسندگان [English] | ||
| A. Shariat1؛ G. Karimzadeh2؛ M.H. Assareh3؛ J. Hadian4 | ||
| 1Ph.D., Department of Plant Breeding, Faculty of Agriculture, Tarbiat Modares University, Tehran, I.R. Iran. | ||
| 2Assoc. Prof., Department of Plant Breeding, Faculty of Agriculture, Tarbiat Modares University, Tehran, I.R. Iran | ||
| 3Prof., Research Institute of Forests and Rangelands of Iran, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran. | ||
| 4Assoc. Prof., Department of Agricultural Engineering, Medicinal Plants and Drug Research Institute, Shahid Beheshti University, Tehran, I.R. Iran | ||
| چکیده [English] | ||
| Savory (Satureja khuzistanica) is one of the nine endemic species which have phenolic compounds such as carvacrole and thymol in its essential oil and rosmarinic and other phenolic acids in its extract, has strong antioxidant and antimicrobial properties and remarkable effects on several diseases. Drought stress was induced by stopping irrigation at flowering stage. Samples were taken five times with three interval days and several physiological traits were measured. For oil extraction, Clevenger apparatus was used. For determination of essential oil’s components and methanolic extract compounds, GC, GC/MS and HPTLC were utilized. Profiling of volatiles using GC/MS, showed an increasing-decreasing trend at major phenolic and terpenes compounds such as thymol, γ- Terpinene, p- Cymene, Rosmarinic acid and Caffeic acid. Drought stress also led to a significant increase in oil yield, soluble sugars and proline as well as a significant reduction in leaf water potential, relative water content and pigments. Metabolite profiling of Satureja khuzistanica represents the strategies employed by savory in generating different biochemical phenotype. Applicable results of this study for increasing product quality is effective application of drought stress before harvesting. The results verified that drought stress affected physiological characteristics and secondary metabolism of savory which were useful for future work by using metabolic engineering focusing on important species to increase main compounds, such as carvacrol and rosmarinic acid and caffeic acid. | ||
| کلیدواژهها [English] | ||
| Drought Stress, oil yield, Savory, Secondary metabolites | ||
| مراجع | ||
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