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A Molecular and Serological Study on Visceral Leishmaniasis in Asymptomatic Stray Dogs in Mashhad, Iran | ||
| Archives of Razi Institute | ||
| مقاله 3، دوره 72، شماره 4، اسفند 2017، صفحه 231-235 اصل مقاله (112.5 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2017.113300 | ||
| نویسندگان | ||
| S. Sabzavari1؛ G. Razmi* 1؛ A. Naghibi1؛ J. Khoshnegah2 | ||
| 1Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| 2Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| چکیده | ||
| Zoonotic visceral leishmaniasis is caused by Leishmania infantum (L. infantum),and its major reservoir hosts are domestic dogs, most of which are asymptomatic. This study aimed to detect L. infantum spp. in asymptomatic stray dogs by molecular and serological methods in Mashhad, Iran, during 2011-12. In this study, 94 asymptomatic stray dogs were randomly selected and their blood samples were collected for indirect fluorescent antibody testing. Furthermore, tissue samples from all the L. infantum seropositive stray dogs were examined using semi-nested polymerase chain reaction (PCR). Accordding to the results, 11.7 %(11/94) of the dogs were L. infantum seropositive. The PCR positivity rate of L. infantum was 63.6% (7/11) in at least one of the collected specimens of the seropositive dogs. The L. infantum kinetoplast DNA (kDNA) was detected in the liver of 36% (4/11), the spleen of 27% (3/11), and the skin of 54.5 %(6/11) of the stray dogs. In this study, based on the molecular and serological examinations, visceral leishmaniasis infection among the stray dogs in Mashhad was confirmed. | ||
| کلیدواژهها | ||
| Leishmania Infantum؛ Stray dogs؛ Mashhad | ||
| عنوان مقاله [English] | ||
| بررسی سرولوژی و ملکولی لیشمانیوز احشایی در سگهای ولگرد فاقد علائم در شهر مشهد | ||
| نویسندگان [English] | ||
| صدف سبزواری1؛ غلامرضا رزمی1؛ ابوالقاسم نقیبی1؛ جواد خوش نگاه2 | ||
| 1گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه فردوسی مشهد، مشهد، ایران | ||
| 2گروه علوم بالینی، دانشکده دامپزشکی، دانشگاه فردوسی مشهد، مشهد، ایران | ||
| چکیده [English] | ||
| بیماری لیشمانیوز احشایی ناشی از لیشمانیا انفانتوم بوده و سگهای اهلی به عنوان مخزن بیماری شناسایی شده اند. بسیاری از سگهای آلوده فاقد علائم بالینی بیماری بوده و به عنوان مخزن بیماری شناخته میشوند . هدف این مطالعه بررسی سرولوژی و ملکولی لیشمانیوز احشایی در سگهای ولگرد شهرستان مشهد میباشد. از سال 1390 تا 1392، تعداد 94 قلاده سگ بطور اتفاقی انتخاب و نمونههای خون برای آزمایش ایمونوفلورسنت غیر مستقیم جمع آوری شدند. علاوه بر این نمونههای بافتی جمع آوری شده سگهای ولگرد سرم مثبت جمع آوری شده با روش seminested-PCR بررسی ملکولی شدند. در این بررسی نمونههای سرمی 11 قلاده سگ ( 7/11%)، واجد عیار سرمی مثبت بر علیه لیشمانیا اینفانتوم بودند. همچنین آلودگی به لیشمانیا اینفانتوم با روش seminested-PCR در 7 قلاده از سگهای ولگرد سرم مثبت (6/63% ) تایید گردید. وجود kDNAلیشمانیا اینفانتوم به ترتیب در 36% بافت کبد، 27% بافت طحال و 5/54% بافت پوست سگهای ولگرد سرم مثبت تعیین شدند. در این مطالعه بر پایه نتایج سرولوژی و ملکولی وجود آلودگی به لیشمانیا اینفانتوم دربین سگهای ولگرد فاقد علائم شهر مشهد تعیین گردید. | ||
| کلیدواژهها [English] | ||
| لیشمانیا اینفانتوم, سگ ولگرد, شهر مشهد | ||
| مراجع | ||
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