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Effects of Eucalyptus camaldulesis aquatic leaf extract on Sorghum halapense seed germination, antioxidants enzyme and α-amylase enzyme activities | ||
| علوم و فناوری بذر ایران | ||
| Article 7, Volume 6, Issue 1 - Serial Number 11, June 2017, Pages 69-77 PDF (512.78 K) | ||
| Document Type: Original Article | ||
| DOI: 10.22034/ijsst.2017.113289 | ||
| Authors | ||
| R. Farhoudi* 1; F. Por Hassan2 | ||
| 1Associate Professor/ Department of Weed Science, Shoushtar branch, Islamic Azad University, Shoushtar, Iran. | ||
| 2Post-Graduated/ Department of Weed Science, Shoushtar branch, Islamic Azad University, Shoushtar, Iran. | ||
| Abstract | ||
| In order to evaluate the allelopatic potential of Eucalyptus camaldulesis aquatic extract on antioxidant enzyme activities, cell membrane damage and α-amylase enzyme activity of S. halapense, thisexperiments was conducted in Islamic Azad University, Shoushtar branch at 2012. The experiment was laid out according to a Completely Randomized Design with five replications and six treatments were various concentration of E. camaldulesis aquatic extract (0, 10, 20, 30, 40, 50 and 60%). The results indicated E. camaldulesis aquatic extract application exhibited gradual rise inhibitory effect on seed germination, seedling fresh weight, antioxidants enzymes activities, and α-amylase enzyme activity. Contrary to the previous traits, elevated malondialdehyde concentration and seedling fatty acid in S. halapense seedlings were detected. The minimum α-amylase enzyme activity (3.1 and 3.5 nmol prot -1 min-2 ), seedling fresh weight (0.1 and 0.8 mg) and seed germination (54% and 42 %) showed in 40% and 50% Eucalyptus camaldulesis aquatic extract. The highest fatty acid (23.6% and 23.1%) was noted at 40% and 50% Eucalyptus camaldulesis aquatic extract. In conclusion, E. camaldulesis aquatic extract decreased seedling growth, α-amylase enzyme activity and cell membrane stability of S. halapense seedling. | ||
| Keywords | ||
| Allelopathy; catalase; Fatty acid; germination; malondialdehyde; peroxidase | ||
| References | ||
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