Babai Gourchinloo, Z., Kefayat, M., Namavari, M. (2017). Comparison of virulence and histopathological changes of NC-1 and NC-SweB1 isolates of Neospora caninum in in chicken embryonated eggs. , 30(4), 92-99.
Z. Babai Gourchinloo; M. Kefayat; Mehdi Namavari. "Comparison of virulence and histopathological changes of NC-1 and NC-SweB1 isolates of Neospora caninum in in chicken embryonated eggs". , 30, 4, 2017, 92-99.
Babai Gourchinloo, Z., Kefayat, M., Namavari, M. (2017). 'Comparison of virulence and histopathological changes of NC-1 and NC-SweB1 isolates of Neospora caninum in in chicken embryonated eggs', , 30(4), pp. 92-99.
Babai Gourchinloo, Z., Kefayat, M., Namavari, M. Comparison of virulence and histopathological changes of NC-1 and NC-SweB1 isolates of Neospora caninum in in chicken embryonated eggs. , 2017; 30(4): 92-99.

Comparison of virulence and histopathological changes of NC-1 and NC-SweB1 isolates of Neospora caninum in in chicken embryonated eggs

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 11, Volume 30, Issue 4, December 2017, Pages 92-99    PDF (2.31 M)
Document Type: Full Research Paper
Authors
Z. Babai Gourchinloo1; M. Kefayat* 2; Mehdi Namavari3
1Veterinary Faculty, Islamic Azad University, Karaj, Iran.
2Department of Pathobiology, Veterinary Faculty, Islamic Azad University, Karaj, Iran
3Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran.
Abstract
Inspite of limited data on the role of birds in the field of infection with Neospora caninum, there is no report about the role of chicken in the field of infection with this parasite. In this study, two isolates of NC-1 and NC-SewB1 in Ross embryonated eggs were used. For each of the isolates, six different dilutions (10, 102 ,103,104,105,106) were prepared for infection. Group 7 was considered as control group. Insemination was conducted in chorioallantoic liquid of embryonated eggs in 9 days. Rates of death, macroscopic changes, and tissue histopathology of dead chicken and chickens that were kept one month after hatching were evaluated. The results showed that the mortality rate in both NC-1 and NC-SweB1 was dependent on amount of inseminated tachyzoite. Thus, in greater quantities of tachyzoite, rates of death and histopathological lesions were higher. The comparison of death of embryonated eggs in isolated NC-1 and NC-SweB1 both using Fisher test showed that the total mortality rate in both groups was statistically significant. According to the average lethal dose (LD50) that was 101.5 and 101.1 for NC-1 and NC-SweB1, respectively, we can conclude that virulence of the NC-1 is higher than NC-SweB1. The results of this study showed that the study of embryonated eggs can be a valuable approach to test live and attenuated vaccines in the in vivo environments.
Keywords
Neospora caninum; Embryonated eggs; NC-1; NC-SweB1
References

1. Corbelini, L.G., Colodel, E.M. and Driemeier, D (2001). Granulomatous encephalitis in a neurologically impaired goat kid associated with degeneration of Neospora caninum tissue cysts. Journal of Veterinary Diagnostic Investigation, 13(5): 416-419,
2. Dubey, J.P., Carpenter, J.L. Speer, C.A. Topper, M.J. and Uggla, A (1988a). Newly recognized fatal protozoan disease of dogs. J Am Vet Med Assoc, 192:1269-1285.
3. Dubey, JP. and. Lindsay, D.S (1993). "Neosporosis."Parasitol. Today, 9:452–458.
4. Dubey, JP., Lindsay, D.S. Adams, D.S. Gay, J.M. Basler, T.V. Blagburn, B.L. and Thulliez, P (1996). "Serologic responses of cattle and other animals infected with Neospora caninum."Am. J. Vet. Res, 57: 329–336.
5. Dubey, J.P (2003b). "Review of Neospora caninum and Neosporosis in animal." Korean. J. Parasitology, 41(1): 1-16.
6. Dubey, J.P. and Schares, G (2006). "Diagnosis of Bovine Neosporosis."Vet. Parasitol, 140: 1–34.
7. Dubey, JP., Schares, G. and Ortega-Mora L.M (2007). "Epidemiology and control of neosporosis and Neospora caninum." Clin. Microbiol. Rev, 20: 323–367
8. Folkman, J. and Moscona, A (1978). "Role of cell shape in growth control." Nature. 273: 345–349.
9. Furuta, PI., Mineo, T.W.P., Carrasco, A.O., Godoy, G.S. Pinto, A.A. and Machado R.Z (2007). "Neospora caninum infection in birds: experimental infections in chicken and embryonated eggs." Parasitology, 134: 1931– 1939.
10. Goedbloed, E. and Kinyanjui, H (1970) . "Development of African Pathogenic trypanosomes in chicken Embroys" , Emperimental Parasitology, 27(3): 464-478.
11. Khodakaram-Tafti, A., Mansourian, M. Namavari, M.M. and Hosseini, A (2012). Immunohistochemical and polymerase chain reaction studies in Neospora caninum experimentally infected broiler chicken embryonated eggs. Vet Parasitol. 188:10–13.
12. Lindsay, D.S. and Current, W.L (1984). "Complete Development of Isospora Suis of Swine in Chicken Embryos", The Journal of Protozoology,31(1): 152-155.
13. Lindsay DS, Steinberg ,H., Dubielzig, R.R., Semrad,S.D., Konkle, D.M. and Miller, P.E. (1996). Central nervous system neosporosis in a foal. J Vet Diagn Invest. 8(4): 507-510.
14. Mansourian, M., Khodakaram, A. and Namavari,M.M (2009).”Histopathological and clinical investigations in Neospora caninum experimentally infected broiler chicken embryonted egges.”Veterinary Parasitology. 166:185-190.
15. Mello, M.N. and Deane, M.P (1976). Patterns of development of Trypanosoma cruzi in the embryonated chicken egg. Ann. Trop. Med. Parasit. 70: 380–388
16. Moraveji, M., Hosseini,A., Moghaddar, N., Namavari, M.M. and Eskandari, M.H. (2012). Development of latex agglutination test with recombinant NcSAG1 for the rapid detection of antibodies to Neospora caninum in cattle. Veterinary Parasitology.
17. Namazi, F., Oryan, A., Namavari, M.M. and Rahimain,A (2010). "Experimental infection of embryonated eggs of chicken with Besnoitia caprae".Tropical Biomedicine, 27(3):417-423.
18. Namavari,M., Mansourian, M., Khodakaram Tafti, A., Hosseini, M.A., Rahimiyan, A., Khordadmehr, M. And Lotfi, M (2012). Application of chicken embryonated eggs as a new model for evaluating the virulence of Neospora caninum tachyzoites, Comp Clin Pathol, 21:1665–1668.
19. Que, X., Wunderlich, A., Joiner, K.A. and Reed, S.D (2004). Toxopain-1 is critical for infection in a novel chicken embryo model of congenital toxoplasmosis. Infect. Immun. 72:2915–2921.
20. Ramamoorthy, S., Duncan,R., Lindsay, D.S. and Sriranganathan, N (2007). Optimization of the use of C57BL/6 mice as a laboratory animal model for Neospora caninum vaccine studies. Veterinary Parasitology 145: 253-259.
21. Warren, J. and Russ, S.B (1948). Cultivation of toxoplasma in embryonated eggs – an antigen derived from chorioallantoic membrane. Proc. Soc. Exp. Biol. Med. 67:85–89
22. Wunderlin, E., Wild, P. and Eckert, J (1997). Comparative reproduction of Cryptosporidium baileyi in embryonated eggs and in chickens. Parasitol. Res, 83:712–7.

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