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امکانسنجی کاربرد چند استروئید گیاهی بهعنوان حشرهکشهای زیستی از طریق بررسی مدلهای برهمکنش آنها با گیرندههای کداستروئیدی کرم غوزهی Helicoverpa armigera و بالتوری سبز Chrysoperla carnea | ||
| مهار زیستی در گیاهپزشکی | ||
| مقاله 5، دوره 4، شماره 2 - شماره پیاپی 8، اسفند 1395، صفحه 55-69 اصل مقاله (471.27 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2017.112923 | ||
| نویسندگان | ||
| آرزو حیدرزاده* 1؛ اروج ولیزادگان2؛ محمود جوگار3 | ||
| 1دانشجوی دکتری تحصصی، دانشگاه کشاورزی دانشگاه ارومیه | ||
| 2گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه ارومیه | ||
| 3دانشکده کشاورزی، دانشگاه ارومیه | ||
| چکیده | ||
| کرم غوزهی پنبه Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) یکی از آفات مهم و کلیدی پنبه در جهان و در ایران است. این گونه دارای دامنهی وسیع میزبانی میباشد و هر ساله خسارت غیر قابل جبرانی را به کشاورزان تحمیل میکند. در زمینهی مبارزه با آفات گیاهی از روشهای مختلفی استفاده میشود. در طی سالیان اخیر محققین در جستجوی یافتن تکنولوژی تولید حشره کشهای بیخطری هستند که دارای خصوصیاتی از قبیل نحوهی اثر انتخابی بیشتر روی حشرات هدف و خطرات زیست محیطی کمتر روی موجودات غیرهدف باشند. در این میان حشرهکشهای مختل کننده پوست اندازی بسیار مورد اقبال واقع شدند. در این مقاله سعی شده تا به استفاده از غربالگری مجازی توسط داکینگ مولکولی مدلهای برهمکنش گیرندههای اکدیزونی و تعدادی استروئید گیاهی که در سالهای 2013 تا 2015 در بانک ترکیبات استروئیدی بهثبت رسیده است، بهکارگیری این ترکیبات در مبارزه با کرم غوزهی Helicoverpa armigera با کمترین اثر سو بر روی بالتوری سبزChrysoperla carnea بهعنوان دشمن طبیعی آن امکانسنجیگردد. مدل گیرندههای اکدیزون آفت و بالتوری سبز طراحی و برآورد کارایی شد. شاخص Z-score، کیفیت مدل گیرنده EcR ساخته شده برای کرم غوزه و بالتوری سبز را بهترتیب معادل روشهای NMR وX-ray Crystallography دانست. نتایج نشان داد که در بین استروئیدهای گیاهی مورد بررسی، استروئیدهای 11β-HYDROXY-20-DEOXYSHIDASTERONE، 2,3,14,20,26-PENTAHYDROXY-6-OXO-STIGMAST-7-ENE-22,26-LACTOL، CALLECDYSTEROL A,B,C و GLUTINOSTERONE کمترین ثابت بازداری (Ki)، منفیترین انرژی پیوند و کمترین میزان تغییرات RMSD در هنگام اتصال بهگیرنده هدف اکدیزون آفت را نشان دادهاند و همچنین در اتصال به گیرندههای اکدیزونی بالتوری سبز بسیار کم اثر ظاهر شدند. لذا استروئیدها گیاهی ذکر شده از پتانسیل بالایی برای کنترل کرم غوزه برخوردار هستند و تأثیر بسیار کمی در برهم زدن سیکل زندگی بالتوری سبز خواهند داشت. | ||
| کلیدواژهها | ||
| داکینگ مولکولی؛ کرم غوزهی پنبه؛ گیرندهی اکدیزونی؛ همولوژی مدل | ||
| عنوان مقاله [English] | ||
| Feasibility study on the use of several phytoecdysteroids as biopesticides via evaluation of their interaction models with Ecdysone receptors of Helicoverpa armigera and Chrysoperla carnea | ||
| نویسندگان [English] | ||
| Arezoo Heydarzade1؛ Orouj Valizadegan2؛ Mahmood Jokar3 | ||
| چکیده [English] | ||
| Helicoverpa armigera (Lepidoptera: Noctuidae) is one of the important pests of cotton with wide host range and cause an irrecoverable damage in the agriculture system in Iran. In the last decade, researchers probe a new technology to produce safe pesticides with some properties like selective effect on target pests and fewer environmental risks on non-target organisms. One of the most popular pesticides is disruptive molting (IGRs) which use widespread in farms. The aim of this article is to evaluate the interaction between some phytoecdysteroids which submitted in data base, during 2013-2015, and Ecdysone receptors in order to control of H. armigera with less adverse effect on Chrysoperla carnea as a natural enemy by molecular Docking. Ecdysone receptor models of H. armigera and C. carnea were designed and their efficacies were estimated. According to Z-score index, qualities of EcR receptors made for H. armigera and C. carnea were equivalent to NMR and X-ray Crystallography methods respectively. Result shown that among tested phytoecdysteroids, 11β-Hydroxy-20-Deoxyshidasterone; 2,3,14,20,26-PentaHydroxy-6-OXO-Stigmast-7-ENE-22.26-Lactol; Callecdysterol A,B,C and Glutinosterone, had minimum inhibition constant (Ki), the most negative bonding energy and minimum changes in RMSD in binding to target pest ecdysone receptor .In conclusion, the mentioned phytoecdysteroids, have a potential to control of H. armigera with fewer side effects on C. carnea life cycle. | ||
| کلیدواژهها [English] | ||
| Molecular Docking, Helicoverpa armigera؛ EcR؛ Homology Model | ||
| مراجع | ||
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