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بررسی خصوصیات فیتوشیمیایی، آنتی‌اکسیدانی و اثر عوامل اکولوژیکی بر پراکنش Salvia aethiopis L. در منطقه هزارجریب بهشهر | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 8، دوره 33، شماره 2 - شماره پیاپی 82، تیر 1396، صفحه 256-267 اصل مقاله (293.91 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2017.107622.1850 | ||
| نویسندگان | ||
| عیسی جعفری فوتمی* 1؛ موسی اکبرلو2؛ معصومه مازندرانی3؛ محمدرحیم فروزه4 | ||
| 1دانشجوی دکترا، گروه علوم مرتع، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران | ||
| 2دانشیار، گروه علوم مرتع، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران | ||
| 3استادیار، گروه زیست‌شناسی، واحد گرگان، دانشگاه آزاد اسلامی، گرگان، ایران | ||
| 4استادیار، گروه علوم مرتع، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران | ||
| چکیده | ||
| این مطالعه با هدف بررسی ویژگی‌های فیتوشیمیایی، فنل و فلاونویید کل و آنتی‌اکسیدانی گیاه دارویی Salvia aethiopis L. و مهمترین عوامل محیطی مؤثر در پراکنش در منطقه هزارجریب بهشهر انجام شد. 5 نمونه سرشاخه‌های گلدار گیاه در اوایل خردادماه 1395 از ارتفاعات منطقه جمع‌آوری و به‌روش تقطیر با آب (طرح کلونجر)، اسانس‌گیری شد. مشخصات مربوط به رویشگاه یادداشت‌برداری گردید و همین‌طور 5 نمونه خاک برای ارزیابی خصوصیات فیزیکی و شیمیایی خاک برداشت شد. ترکیب‌های تشکیل‌دهنده اسانس با استفاده از دستگاه‌های کروماتوگراف گازی (GC) و گاز کروماتوگراف متصل شده به طیف‌سنج جرمی (GC/MS) شناسایی شدند. استخراج عصاره اتانولی نمونه‌ها به روش پرکولاسیون، ارزیابی فیتوشیمیایی (فنول و فلاونویید کل) با استفاده از روش‌های اسپکتروفتومتری و بررسی عملکرد آنتی‌اکسیدانی نمونه‌ها نیز با استفاده از آزمون DPPH ارزیابی گردیدند. با استفاده از روش PCA نیز مهمترین عوامل مؤثر در پراکنش گونه مورد نظر مشخص شدند. در اسانس 7 ترکیب شناسایی شد. بتا-کاریوفیلن (28.4%) و 1،6-سیکلودکادین (26%) ترکیب‌های اصلی اسانس گل را تشکیل می‌دهند و بتا-کاریوفیلن (30.3%) و کوپائن (23.3%) مهمترین ترکیب‌های تشکیل‌دهنده برگ هستند. فنل کل، فلاونوئید کل و آنتی‌اکسیدان موجود در گل از برگ گیاه بیشتر است. عوامل محیطی تأثیرگذار بر پراکنش گیاه Salvia aethiopis شامل ارتفاع، آهک، ماده آلی، اسیدیته، کلسیم، سیلت، شن و جهت شیب هستند. | ||
| کلیدواژه‌ها | ||
| Salvia aethiopis L؛ عوامل محیطی؛ PCA؛ هزارجریب؛ فعالیت آنتی‌اکسیدانی | ||
| عنوان مقاله [English] | ||
| An investigation of phytochemical and antioxidant characteristics of Salvia aethiopis L. and ecological factors affecting its distribution in Hezarjarib, Behshahr Province | ||
| نویسندگان [English] | ||
| I. Jafari Footami1؛ M. Akbarlo2؛ M. Mazandarani3؛ M.R. Foroozeh4 | ||
| 1Ph.D. Student, Department of Rangeland, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran | ||
| 2Department of Rangeland, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran | ||
| 3Department of Biology, Islamic Azad University, Gorgan Branch, Gorgan, Iran | ||
| 4Department of Rangeland, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran | ||
| چکیده [English] | ||
| The aim of this study was to investigate the essential oil composition, total phenol and flavonoid, antioxidant activities and the effect of ecological factors on distribution of Salvia aethiopis L.in Hezar Jerib region of Behshahr, Mazandaran province. Five samples of flowering branches were collected in early June 2016 from the highlands of the study area and extracted with water distillation method. In addition, five soil samples were taken to evaluate the physical and chemical properties of soil. Oil composition was identified by (GC) and (GC/MS). The ethanol samples were extracted by percolation method. Total phenol (TP) and total flavonoids (TF) of methanolic extracts were measured by spectrophotometry and antioxidant activity was measured by scavenging free radicals of DPPH. The most important of ecological factors affecting the distribution of species were identified by PCA method. Seven compounds were identified in the essential oil. The major components in the flower essential oil were β-caryophyllene (28.64) and 1,6-cyclodecadiene (24.98) while β-caryophyllene (30.3) and copaene (23.3) were the major components in the leaf essential oil. Ecological factors affecting the distribution of Salvia aethiopis werealtitude, Caco3, organic matter, pH, calcium, silt, sand, and direction. | ||
| کلیدواژه‌ها [English] | ||
| Salvia aethiopis L, ecological factors, PCA, Hezar Jerib area, Antioxidant activity | ||
| مراجع | ||
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