| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شمارهها | 3,168 |
| تعداد مقالات | 35,197 |
| تعداد مشاهده مقاله | 50,944,368 |
| تعداد دریافت فایل اصل مقاله | 90,949,918 |
باززایی غیرمستقیم کیکم ترکمنی (Acer monospessulanum) در شرایط درون شیشهای | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 1، دوره 25، شماره 1 - شماره پیاپی 49، خرداد 1396، صفحه 1-12 اصل مقاله (510.72 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2017.109811 | ||
| نویسندگان | ||
| عباس صفرنژاد* 1؛ هادی درودی2 | ||
| 1عضو هیات علمی مرکز تحقیقات کشاورزی و منابع طبیعی خراسان رضوی، سازمان تحقیقات، آموزش و ترویج کشاورزی | ||
| 2دکترای جنگلداری، بخش تحقیقات کشت بافت، پژوهشکده بیوتکنولوژی کشاورزی ایران، سازمان تحقیقات، آموزش و ترویج کشاورزی، مشهد | ||
| چکیده | ||
| گونه کیکم یکی از گونه های مهم جنس Acer از خانواده Aceraceae میباشد. زیرگونه turcomanicum در جنگلهای ناحیه رویشی ایران تورانی بهعنوان گونـه همـراه ارس در ارتفاعات استانهـای خراسـان رضـوی و شـمالی پراکنش دارد. سال بذردهى این گونه هر سه سال یکبار است. قوه نامیه بذر آن پایین و تعداد بذرهای رسیده و سالم کم میباشد. بذرهای این گونه دارای خواب بوده و قوه نامیه آن پس از طى دوره سرمادهى کاهش مییابد. متأسفانه، بسیاری از رویشگاههای این گونه تحت تأثیر چرای دام و دخالتهای انسانی با تخریب زیادی مواجه شده است. بهدلیل مشکلات تکثیر جنسی، استفاده از روشهای غیرجنسی بهمنظور تولید نهالهای با کیفیت مطلوب میتواند در احیاء رویشگاههای این گونه بسیار راهگشا باشد. یکی از راههای مطمئن تولید نهال، تکثیر غیرجنسی از طریق ریزازدیادی است. به این منظور ریزنمونهها از رویشگاه آن تهیه و باززایی انجام شد. القاء کالوس در محیطهای هورمونی مختلف انجام و غلظتهای مختلف هورمونی برای باززایی کالوس بررسی شد. از بین محیطهای مورد بررسی، باززایی غیرمستقیم از کالوس تنها در غلظتهای 1/0 و 5/0 میلیگرم در لیتر هورمون رشد گیاهی تیدیازورون (TDZ) انجام شد. تعداد، رشد طولی و شادابی ساقهچهها تحت تأثیر غلظتهای مختلف هورمون TDZ بررسی شد و تیمار 5/0 میلیگرم در لیتر هورمون TDZ با متوسط 16/4 عدد بیشترین تعداد ساقهزایی را داشت. از نظر شادابی و ارتفاع ساقهچهها تفاوت معنیداری بین تیمارها وجود نداشت. بهطورکلی محیط پایه MS و هورمون TDZ برای باززایی کالوس گونه کیکم ترکمنی مناسب میباشد. گیاهچههای باززایی شده بهمنظور سازگاری به گلدان منتقل و بعد به خاک انتقال یافتند. | ||
| کلیدواژهها | ||
| افراکیکم؛ باززایی؛ کالوس؛ TDZ | ||
| عنوان مقاله [English] | ||
| Indirect regeneration of Acer monospessulanum by in vitro techniques | ||
| نویسندگان [English] | ||
| A. Safarnejad1؛ H. Darroudi2 | ||
| 1Faculty member of Khorasan Razavi Agricultural and Natural Resources Research Center or Mashhad Branch, Education and Extension Organization (AREEO) | ||
| 2PhD, Mashhad Branch, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, I.R. Iran | ||
| چکیده [English] | ||
| Acer monospessulanum is an important species of the genus Acer from Aceraceae family. Turcamanicum subspecies spreads in Irano-Turanian region as associated plant of juniper forests in highlands of Razavi Khorasan province and northern part of the province. Its mast year is every three years, germination rate of seeds and number of healthy matured seeds is low. Also, the seeds have dormancy period and their germination percentage is decreased after chilling periods. Unfortunately, most of the species habitats are under intense grazing and human intervention effects, so that the species has been encountered with severe destruction. Due to sexual reproduction problems, application of vegetative propagation methods could be advantageous in high quality seedling production and reclamation of the habitats. One of seedling production methods is propagation using in vitro techniques. For this purpose, explants were collected from their natural habitats, and their regeneration was assessed in different hormonal treatments. Embryogenesis percentage in different hormonal treatments was investigated. Applying different hormonal treatments, callus regeneration was observed only in 0.1 and 0.5 mg/l of TDZ hormone concentration. Shoot number, shoot growth and shoot vigority were affected by different concentrations of TDZ hormones. MS media supplemented by 0.5 mg/l TDZ had the highest shoot production. But vigority and shoot length were not significantly different by utilizing different hormonal treatments. Finally, MS basal media and TDZ hormone are suitable for regeneration from callus in the valuable tree species. | ||
| کلیدواژهها [English] | ||
| Acer monospessulanum, Callus, regeneration, TDZ | ||
| مراجع | ||
|
- Abbasi, B., Saxena, P. K., Murch, S. J. and Liu, C.Z., 2007. Echinacea biotechnology: Challenges and opportunities. In vitro Cellular and Developmental Biology-Plant, 43: 481-492. - Bhaskaran, S. and Smith, R.H. 1990. Regeneration in cereal tissue culture: A review. Crop Science, 30: 1329- 1336. - Bonga, J. M. and von Aderkas, P., 1992. Clonal Propagation. In: "In vitro Culture of Trees", (Eds.): Bonga, J. M. and von Aderkas, P., Kluwer Academic Publishers, Dordrecht, PP. 76-104. - Durkovic, J., 2003. Regeneration of Acer caudatifolium Hayata plantlets from juvenile explants. Plant Cell Report, 21:1060–1064. - Emam, M., 2005: Micropropagation of Acer cinerasens by shoot tip culture. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 13: 37- 51. - Emam, M., Shahrzad, Sh., Naraghi, T.S., Khanhasani, M. and Hamzepoor, Y., 2006. Regeneration of Acer cinerasens through embryo culture. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 14: 170-174. - Gallo-Meagher, M., English, R.G. and Abouzid, A., 2000. Thidiazuron stimulates shoot regeneration of sugarcane embryogenic callus. In Vitro Cellular and Developmental Biology-Plant, 36: 37-40. - Guo, B., Abbasi,B, H., Zeb, A., Xu, L. L., and Wei, Y. H., 2011. Thidiazuron: A multi-dimensional plant growth regulator. African Journal of Biotechnology, 10: 8984-9000 - Han, Y., Jin, X., Wu, F. and Zhang, G., 2011. Genotypic differences in callus induction and plant regeneration from mature embryos of barley (Hordeum vulgare L.). JZUS, 12(5): 399-407. - Huetteman, C. A. and Preece, J.E., 1993. Thidiazuron: A potent cytokinin for woody plant tissue culture. Plant Cell Tissue and Organ Culture. 33(2): 105-119. - Husain, M.K., Anis, M., and Shahzad, A., 2007. In vitro propagation of Indian Kino (Pterocarpus marsupium Roxb.) using Thidiazuron. In vitro Cellular and Developmental Biology-Plant, 43: 59-64. - Latteir, J.D., Touchell, D.H., Ranney, T.G. and Smith, J.C., 2013. Micropropagation and polyploidy induction of Acer platanoides Crimson sentry. Journal of Environment and Horticulture , 31:246–252. - Madani, G., Ghobadi, S., Seyed Tabatabaei, B,E., Talebi, M. and Yamchi, A., 2013. Effect of plant growth regulators and explant types on regeneration and micropropagation of a commercial strawberry cultivar (Fragaria ×ananassa cv. Selva). Journal of Science and Technology Greenhouse Culture, 15(4): 111 – 121. - Mok, M. C., Mok, D., Turner, J. and Mujer, C., 1987. Biological and biochemical effects of cytokinin active phenylurea derivatives in tissue culture systems. HortScience, 22:1194-1197. - Mozaffarian, V., 2004. Trees and shrubs of Iran, Res Inst of forest and rangeland, Tehran, 1003 pp. - Mroginski, E., Rey, H.Y., Gonzalez, A.M. and Mroginski, L.A., 2004. Thidiazuron promotes in vitro plant regeneration of Arachis correntina (Leguminosae) via organogenesis. Journal of Plant Growth Regulators, 23: 129-134. - Murthy, B., Murch, S. and Saxena, P. K., 1998. Thidiazuron: A potent regulator of in vitro plant morphogenesis. In vitro Cellular and Developmental Biology-Plant, 34:267-275. - Nasiri, M., 2008. Investigation of suitable seed germination enhancement and breaking seed dormancy treatment of Montpellier maple (Acer monospessulanum L.). Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 16: 94-105. - Neto, V.B.P., Mota, T.R. and Otoni, W.C., 2003. Direct organogenesis from hypocotyl-derived explants of annatto (Bixaorellana). Plant Cell Tissue and Organ Culture, 75: 159-167. - O’Connor, A., Hubstenberger, J., Killough, C., VanLeeuwen, D. and St Hilaire, R., 2007. In vitro propagation of Acer grandidentatum Nutt. In vitro Cellular and Developmental Biology-Plant, 43: 40-50. - Radhika, K., Sujatha, M. and Rao, N, T., 2006. Thidiazuron stimulates adventitious shoot regeneration in different safflower explants. Biologia Plantarum, 50: 174-179 - Sabeti, H., 1992. Forests, Trees and Shrubs of Iran. Yazd University Press, Yazd, 890pp . - Saeedi Heidari, A. and Safarnejad, A., 2015. Micropropagation of Acer monospessulanum through tissue culture. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 23: 237-246. - Safarnejad, A., 2015. Effects of growth regulators on in vitro regeneration of Ziziphus jujube. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 23(1): 40 – 48. - Sagheb Talebi, Kh., Sajedi, T., and Yazdian, F., 2004. Take the Forests of Iran. Research Institute of Forests and Rangelands, Tehran, 270pp. - Salavati, Gh., Payamnoor, V., Kavoosi, M.R. and Ali-Arab, A., 2013. Storage behavior and desiccation sensitivity of seeds in Acer monspessulanum L. Journal of Forest and Wood Products, 66: 293- 304. - Sayed Tabatabaei, B.E. and Omidi, M., 2009. Plant Cell and Tissue Culture. University of Tehran Press. Tehran. (in persian). 368pp. - Sotiropoulos, T.E., Mouhtaridou, G.N., Thomidis, T., Tsirakoglou, V., Dimassi, K.N. and Therios, I.N., 2005. Effects of different N-sources on growth, nutritional status, chlorophyll content, and photosynthetic parameters of shoots of the apple rootstock MM 106 cultured In vitro. Biologia Plantarum, 49: 2. 297-299. - Sujatha, G. and Ranjitha Kumari, B.D., 2007. High-frequency shoots multiplication in Artemisia vulgaris L. using thidiazuron. Plant Biotechnology Report, 1: 149-154. - Tomas, W., Motyka, V., Strnad, M. and Schmulling, T., 2001. Regulation of plant growth by cytokinins. Journal of Horticultural Science, 6: 36-39. - Vlašínová, H. and Havel, L., 1999. Continuous somatic embryogenesis in Japanese Maple (Acer palmatum Thunb). Journal of Plant Physiology, 154:212-218. - Wilhelm, E., 1999. Micropropagation of juvenile sycamore maple via adventitious shoot formation by use of thidiazuron. Plant Cell, Tissue and Organ Culture, 57:57-60. - Yucesan, B., Turker, A.U., and Gurel, E., 2007. TDZ-induced high frequency plant regeneration through multiple shoot formation in witloof chicory (Cichorium intybus L.). Plant Cell Tissue and Organ Culture, 91: 243-250. | ||
|
آمار تعداد مشاهده مقاله: 822 تعداد دریافت فایل اصل مقاله: 686 |
||