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بررسی اثر فلز روی بر بیان ژن لیمونن سینتاز در زیره سبز با PCR در زمان واقعی | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 3، دوره 24، شماره 2 - شماره پیاپی 48، آذر 1395، صفحه 190-198 اصل مقاله (228.58 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2016.109409 | ||
| نویسندگان | ||
| محمد طغرلی1؛ جعفر وطندوست* 2؛ ملک حسین اسدی3 | ||
| 1دانشجوی کارشناسی ارشد، بیوتکنولوژی کشاورزی، دانشگاه آزاد اسلامی، واحد سبزوار | ||
| 2نویسنده مسئول مکاتبات، استادیار، گروه زیستشناسی، دانشگاه حکیم سبزواری، سبزوار | ||
| 3استادیار، گروه بیوتکنولوژی، پژوهشگاه علوم و تکنولوژی پیشرفته و علوم محیطی، دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان | ||
| چکیده | ||
| روی بهعنوان یک عنصر ضروری برای رشد و نمو گیاهان، نقش مهمی در بسیاری از فرایندهای متابولیکی گیاهان ازجمله بیان ژن لیمونن سینتاز برعهده دارد. لیمونن سینتاز بهعنوان یک آنزیم کلیدی در مسیر بیوسنتز روغنهای فرار میباشد که در واکنشهای دفاعی گیاهان نقش دارد. از اینرو در این پژوهش اثر غلظتهای مختلف روی بر بیان ژن لیمونن سینتاز در زیره سبز و در قالب طرح کاملاً تصادفی بررسی شد. بعد از استخراج RNA و سنتز cDNA، بیان ژن با روش Real time PCR بررسی شد. نتایج مقایسه میانگین میزان بیان ژن با آزمون چند دامنهای دانکن در غلظتهای مختلف نشان داد که در گیاهان تیمار شده، افزایش غلظت روی تا 100 میکرومولار با افزایش بیان ژن لیمونن سینتاز رابطه مستقیم دارد. با توجه بهنقش آنزیم لیمونن سینتاز، افزایش بیان آن میتواند از طریق افزایش تولید مونوترپنها در گیاه باعث تولید بیشتر روغنهای فرار و در نتیجه محافظت گیاه در مقابل علفخوارها شود. | ||
| کلیدواژهها | ||
| بیان ژن؛ روی؛ زیره سبز؛ لیمونن سینتاز؛ real time PCR | ||
| عنوان مقاله [English] | ||
| Study of zinc effect on limonene synthase gene expression in cumin by Real Time PCR | ||
| نویسندگان [English] | ||
| Mohammad Toghroli1؛ Jafar Vatandoost2؛ M.H. Asadi3 | ||
| 1M.Sc., Department of Biotechnology, Faculty of Agriculture, Islamic Azad University, Sabzevar, I.R. Iran | ||
| 2Corresponding author Assis. Prof., Department of Biology, Faculty of Science, Hakim Sabzevari University, Sabzevar, I.R. Iran, E-mail: j.vatan@hsu.ac.ir | ||
| 3Assis. Prof., Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, I.R. Iran. | ||
| چکیده [English] | ||
| Zinc as an essential element for plant growth, plays an important role in many metabolic processes of plants, such as limonene synthase gene expression pathway. Limonene synthase is considered as an important enzyme in volatile oil biosynthesis that plays significant role in plant defensive reactions. In the present study, limonene synthase gene expression in Cumin was evaluated in presence of different concentration of Zinc elicitors based on a completely randomized design. Total RNA was extracted and cDNA synthesis was performed and Real Time PCR quantity assay method was used for limonene synthase gene expression. Results of the comparison of gene expression with Duncan's multiple range tests in different concentrations showed that in treated plants, there was a direct correlation between increasing of Zinc concentration up to 100 µM and limonene synthase gene expression. Limonene synthase gene expression by increasing production of monoterpens may produce more volatile oils in plant species and thus protects the plant against herbivores. | ||
| کلیدواژهها [English] | ||
| Cuminum cyminum, Gene expression, limonen synthase, real time PCR, Zinc | ||
| مراجع | ||
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