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بررسی تنوع ژنتیکی کاکوتی (Ziziphora tenuior L) با استفاده از نشانگر SCoT | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 2، دوره 24، شماره 2 - شماره پیاپی 48، آذر 1395، صفحه 177-189 اصل مقاله (343.25 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2016.109406 | ||
| نویسندگان | ||
| نوید زمانی* 1؛ وحید زمانی2؛ خالد میرزایی3 | ||
| 1مربی، دانشکده محیطزیست و منابع طبیعی، دانشگاه صنعتی خاتمالانبیا، بهبهان | ||
| 2مربی، دانشگاه ژوزففوریر، دانشکده اکولوژی آلپین، گرونوبل، فرانسه | ||
| 3کارشناس ارشد، دانشگاه کردستان، سنندج | ||
| چکیده | ||
| در این مطالعه از 25 آغازگر SCoT برای مطالعه تنوع ژنتیکی 42 نمونه کاکوتی از مناطق مختلف جغرافیایی ایران استفاده شد. در مجموع 209 قطعه تکثیر شد و 66% از آنها چندشکل بودند. بیشترین قطعات تکثیر شده چندشکل (10) مربوط به آغازگر SCoT-54 بود، و همچنین کمترین تعداد قطعات چندشکل (2) مربوط به آغازگر SCoT-20 بود. متوسط تعداد باند برای هر آغازگر 36/8 و متوسط باندهای چندشکل 53/5 بود. محتوای اطلاعات چندشکلی و قدرت تفکیک (PICD) بین 217/0 در آغازگر SCoT-31 و 07/0 در آغازگر SCoT-30 با میانگین 156/0 متغیر بود. ماتریس تشابه با استفاده از ضریب جاکارد، تجزیه خوشهای با الگوریتم UPGMA و تجزیه به مختصات اصلی انجام شد. براساس تجزیه خوشهای، در ضریب تشابه 7/0 نمونههای کاکوتی به 7 گروه تقسیم شدند. براساس ماتریس تشابه، کمترین شباهت (59/0) مربوط به دو نمونه نائین و یاسوج، و همچنین بیشترین شباهت (98/0) بین دو نمونه از دامغان و گرمسار از یک منطقه جغرافیایی مشابه بود. نتایج حاصل از تجزیه واریانس مولکولی (AMOVA) حکایت از تنوع زیاد در داخل نمونهها داشت. در واقع 89% از واریانس در داخل نمونهها و 11% از واریانس در بین نمونههای کاکوتی بود. در نهایت نتایج نشاندهنده تنوع مناسب در بین ژنوتیپهای مورد مطالعه بود. | ||
| کلیدواژهها | ||
| تجزیه ژنتیکی؛ تشابه ژنتیکی؛ گیاه دارویی؛ نشانگر مولکولی | ||
| عنوان مقاله [English] | ||
| Genetic diversity analysis of Ziziphora tenuior L. using SCoT markers | ||
| نویسندگان [English] | ||
| Navid Zamani1؛ Vahid Zamani2؛ Khaled Mirzaei3 | ||
| 1College of Environment and Natural Resources, Khatam Alanbia University of Technology, Behbahan | ||
| 2Laboratory of Alpine Ecology, Joseph Fourier University, Grenoble, France | ||
| 3M.Sc., University of Kurdistan, Sanandaj, I.R. Iran | ||
| چکیده [English] | ||
| Twenty five SCoT primers were employed to investigate genetic diversity of 42 samples of Ziziphora tenuior from different geographical regions of Iran. A total of 209 fragments were amplified and 66% of them were polymorphic. Most amplified polymorphic fragments (10) were related to a primer named SCoT-54, while another primer named SCoT-20 showed the lowest (2) amplified polymorphic bands. Average number of amplified bands for primers was 8.36, while average number of polymorphic bands was 5.53. Polymorphic information content and discrimination power (PICD) varied between 0.217 in SCoT-31 and 0.074 in SCoT-30 with an average of 0.156. Similarity matrix was generated using Jaccard coefficient, Cluster analysis was carried out by UPGMA algorithm and principle coordinate analysis. According to the cluster analysis within the similarity coefficient of 0.7, Ziziphora samples were divided into 7 groups. Based upon similarity matrix, the lowest similarity (0.59) was found for two samples from Naein and Yasuj. The highest similarity (0.98) found between two samples from Damghan and Garmsar within the same geographical region. Analysis of molecular variance (AMOVA) suggested significant genetic differences within the samples. In fact, 89% of variation occurred within the species, whereas 11% of the variation was observed among the samples of Ziziphora tenuior. The results finally indicated moderate genetic diversity among the studied genotypes. | ||
| کلیدواژهها [English] | ||
| genetic analysis, genetic similarity, medicinal plant, molecular marker | ||
| مراجع | ||
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