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بررسی تنوع ژنتیکی کاکوتی (Ziziphora tenuior L) با استفاده از نشانگر SCoT | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 2، دوره 24، شماره 2 - شماره پیاپی 48، آذر 1395، صفحه 177-189 اصل مقاله (343.25 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2016.109406 | ||
| نویسندگان | ||
| نوید زمانی* 1؛ وحید زمانی2؛ خالد میرزایی3 | ||
| 1مربی، دانشکده محیطزیست و منابع طبیعی، دانشگاه صنعتی خاتمالانبیا، بهبهان | ||
| 2مربی، دانشگاه ژوزففوریر، دانشکده اکولوژی آلپین، گرونوبل، فرانسه | ||
| 3کارشناس ارشد، دانشگاه کردستان، سنندج | ||
| چکیده | ||
| در این مطالعه از 25 آغازگر SCoT برای مطالعه تنوع ژنتیکی 42 نمونه کاکوتی از مناطق مختلف جغرافیایی ایران استفاده شد. در مجموع 209 قطعه تکثیر شد و 66% از آنها چندشکل بودند. بیشترین قطعات تکثیر شده چندشکل (10) مربوط به آغازگر SCoT-54 بود، و همچنین کمترین تعداد قطعات چندشکل (2) مربوط به آغازگر SCoT-20 بود. متوسط تعداد باند برای هر آغازگر 36/8 و متوسط باندهای چندشکل 53/5 بود. محتوای اطلاعات چندشکلی و قدرت تفکیک (PICD) بین 217/0 در آغازگر SCoT-31 و 07/0 در آغازگر SCoT-30 با میانگین 156/0 متغیر بود. ماتریس تشابه با استفاده از ضریب جاکارد، تجزیه خوشهای با الگوریتم UPGMA و تجزیه به مختصات اصلی انجام شد. براساس تجزیه خوشهای، در ضریب تشابه 7/0 نمونههای کاکوتی به 7 گروه تقسیم شدند. براساس ماتریس تشابه، کمترین شباهت (59/0) مربوط به دو نمونه نائین و یاسوج، و همچنین بیشترین شباهت (98/0) بین دو نمونه از دامغان و گرمسار از یک منطقه جغرافیایی مشابه بود. نتایج حاصل از تجزیه واریانس مولکولی (AMOVA) حکایت از تنوع زیاد در داخل نمونهها داشت. در واقع 89% از واریانس در داخل نمونهها و 11% از واریانس در بین نمونههای کاکوتی بود. در نهایت نتایج نشاندهنده تنوع مناسب در بین ژنوتیپهای مورد مطالعه بود. | ||
| کلیدواژهها | ||
| تجزیه ژنتیکی؛ تشابه ژنتیکی؛ گیاه دارویی؛ نشانگر مولکولی | ||
| عنوان مقاله [English] | ||
| Genetic diversity analysis of Ziziphora tenuior L. using SCoT markers | ||
| نویسندگان [English] | ||
| Navid Zamani1؛ Vahid Zamani2؛ Khaled Mirzaei3 | ||
| 1College of Environment and Natural Resources, Khatam Alanbia University of Technology, Behbahan | ||
| 2Laboratory of Alpine Ecology, Joseph Fourier University, Grenoble, France | ||
| 3M.Sc., University of Kurdistan, Sanandaj, I.R. Iran | ||
| چکیده [English] | ||
| Twenty five SCoT primers were employed to investigate genetic diversity of 42 samples of Ziziphora tenuior from different geographical regions of Iran. A total of 209 fragments were amplified and 66% of them were polymorphic. Most amplified polymorphic fragments (10) were related to a primer named SCoT-54, while another primer named SCoT-20 showed the lowest (2) amplified polymorphic bands. Average number of amplified bands for primers was 8.36, while average number of polymorphic bands was 5.53. Polymorphic information content and discrimination power (PICD) varied between 0.217 in SCoT-31 and 0.074 in SCoT-30 with an average of 0.156. Similarity matrix was generated using Jaccard coefficient, Cluster analysis was carried out by UPGMA algorithm and principle coordinate analysis. According to the cluster analysis within the similarity coefficient of 0.7, Ziziphora samples were divided into 7 groups. Based upon similarity matrix, the lowest similarity (0.59) was found for two samples from Naein and Yasuj. The highest similarity (0.98) found between two samples from Damghan and Garmsar within the same geographical region. Analysis of molecular variance (AMOVA) suggested significant genetic differences within the samples. In fact, 89% of variation occurred within the species, whereas 11% of the variation was observed among the samples of Ziziphora tenuior. The results finally indicated moderate genetic diversity among the studied genotypes. | ||
| کلیدواژهها [English] | ||
| genetic analysis, genetic similarity, medicinal plant, molecular marker | ||
| مراجع | ||
|
-Aguilar-Støen, M. and Moe, S., 2007. Medicinal plant conservation and management: distribution of wild and cultivated species in eight countries. Biodiversity and Conservation, 16: 1973-1981. -Amirmoradi, B., Talebi, R. and Karami, E., 2012. Comparison of genetic variation and differentiation among annual Cicer species using start codon targeted (SCoT) polymorphism, DAMD-PCR, and ISSR markers. Plant Systematics and Evolution, 298: 1679-1688. -Arif, M., Zaidi, N. W., Singh, Y. P., Haq, Q. M. R., and Singh, U. S., 2009. A comparative analysis of ISSR and RAPD markers for study of genetic diversity in Shisham (Dalbergia sissoo). Plant Molecular Biology Reporter, 27: 488-495. -Belokurova, V.B., 2010. Methods of biotechnology in system of efforts aimed at plant biodiversity preservation (Review). Cytology and Genetics, 44: 174-185. -Collard, B.Y. and Mackill, D., 2009. Start Codon Targeted (SCoT) Polymorphism: A Simple, Novel DNA Marker Technique for Generating Gene-Targeted Markers in Plants. Plant Molecular Biology Reporter, 27: 86-93. -Dakah, A., Zaid, S., Suleiman, M., Abbas, S. and Wink, M., 2014. In vitro propagation of the medicinal plant Ziziphora tenuior L. and evaluation of its antioxidant activity. Saudi Journal of Biological Sciences, 21: 317-323. -Doyle, J.J. and Doyleو J.L., 1987. A rapid DNA isolation procedure from small quantities of fresh leaf tissues. Phytochem Bull., 19:11–15. -Gorji, A.M., Poczai, P., Polgar, Z. and Taller, J., 2011. Efficiency of arbitrarily amplified dominant markers (SCoT, ISSR and RAPD) for diagnostic fingerprinting in tetraploid potato. American Journal of Potato Research, 88: 226-237. -Grime, J., 2002. Benefits of plant diversity to ecosystems: immediate, filter and founder effects. Journal of Ecology, 86: 902-910. -Hatri, Z., Zamani, Z., Nazeri, W. and Tabrizi, L., 2012. Genetic diversity evaluation of a medicinal plant (Ziziphora tenuior.L) of Iran, using RAPD molecular markers. Iranian Journal of Horticulture, 43:337-334. -Hodkinson, T., Waldren, S., Parnell, J.N., Kelleher, C., Salamin, K. and Salamin, N., 2007. DNA banking for plant breeding, biotechnology and biodiversity evaluation. Journal of Plant Research, 120: 17-29. -Jannatdoust, M.,Darvishzadeh, R., Ebrahomi M. A., 2014. Studying Genetic Diversity in Confectionery Sunflower(Helianthus annuus L.) by Using Microsatellite Markers. Crop Biotechnology, 6: 61-72. -Kumar, P., Gupta, V., Misra, A., Modi, D. and Pandey, B., 2009. Potential of molecular markers in plant biotechnology. Plant Omics Journal, 2: 141-162. -Kumar, V., Sharma, S., Kero, S., Sharma, S., Sharma, A.K., Kumar, M. and Bhat, K.V., 2008. Assessment of genetic diversity in common bean (Phaseolus vulgaris L.) germplasm using amplified fragment length polymorphism (AFLP). Scientia Horticulturae, 116:138-143. -Luo, C., He, X. H., Chen, H., Ou, S. J. and Gao, M. P., 2010. Analysis of diversity and relationships among mango cultivars using Start Codon Targeted (SCoT) markers. Biochemical Systematics and Ecology, 38: 1176-1184. -Luo, C., He, X.-H., Chen, H., Hu, Y. and Ou, S.-J., 2012. Genetic relationship and diversity of Mangifera indica L.: revealed through SCoT analysis. Genetic Resources and Crop Evolution, 59: 1505-1515. -Mirzaei, K. and Mirzaghaderi, G., 2015. Genetic diversity analysis of Iranian Nigella sativa L. landraces using SCoT markers and evaluation of adjusted polymorphism information content. Plant Genetic Resources, 1-8. -Mohammadi, S. and Prasanna, B., 2003. Analysis of genetic diversity in crop plants salient statistical tools and considerations. Crop Science, 43: 1235-1248. -Mulpuri, S., Muddanuru, T. and Francis, G., 2013. Start codon targeted (SCoT) polymorphism in toxic and non-toxic accessions of Jatropha curcas L. and development of a codominant SCAR marker. Plant Science, 207: 117-127. -Naeini, A., Khosravi, A., Tadjbakhsh, H., Ghazanfari, T., Yaraee, R. and Shokri, H., 2010. Evaluation of the immunostimulatory activity of Ziziphora tenuior extracts. Comparative Clinical Pathology, 19: 459-463. -Ovesna, J., Polakova, K. and Leisová, L., 2002. DNA analyses and their applications in plant breeding. Czech Journal of Genetics and Plant Breeding, 38: 29-40. -Pecina-Quintero, V., Anaya-López, J.L., Colmenero, A.Z., García, N.M., Colín, C.A.N., Bonilla, J.L.S., Aguilar-Rangel, M.R., Langarica, H.R.G. and Bustamante, D.J.M., 2011. Molecular characterisation of Jatropha curcas L. genetic resources from Chiapas, Mexico through AFLP markers. Biomass and Bioenergy, 35:1897-1905. -Ramanatha Rao, V. and Hodgkin, T., 2002. Genetic diversity and conservation and utilization of plant genetic resources. Plant Cell, Tissue and Organ Culture, 68: 1-19. -Rohlf, F. and Taxonomy, N.P.N., 1998. Multivariate Analysis System, Version 2.02. New York: Exeter Software, Applied Biostatistics Inc. -Tripathi, L. and Tripathi, J.N., 2005. Role of biotechnology in medicinal plants. Tropical Journal of Pharmaceutical Research, 2: 243-253. -Xiong, F., Zhong, R., Han, Z., Jiang, J., He, L., Zhuang, W. and Tang, R., 2011. Start codon targeted polymorphism for evaluation of functional genetic variation and relationships in cultivated peanut (Arachis hypogaea L.) genotypes. Molecular Biology Reports, 38: 3487-3494. | ||
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