Sadeghvaziri, S., Shokrollahi, B., Mohammadi, S. (2017). Ghrelin localization in testicular and epididymis tissues of Ram. , 30(1), 75-81. doi: 10.22034/vj.2017.107798
Saman Sadeghvaziri; Borhan Shokrollahi; Sabah Mohammadi. "Ghrelin localization in testicular and epididymis tissues of Ram". , 30, 1, 2017, 75-81. doi: 10.22034/vj.2017.107798
Sadeghvaziri, S., Shokrollahi, B., Mohammadi, S. (2017). 'Ghrelin localization in testicular and epididymis tissues of Ram', , 30(1), pp. 75-81. doi: 10.22034/vj.2017.107798
Sadeghvaziri, S., Shokrollahi, B., Mohammadi, S. Ghrelin localization in testicular and epididymis tissues of Ram. , 2017; 30(1): 75-81. doi: 10.22034/vj.2017.107798

Ghrelin localization in testicular and epididymis tissues of Ram

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 9, Volume 30, Issue 1, March 2017, Pages 75-81    PDF (3.54 M)
Document Type: Full Research Paper
DOI: 10.22034/vj.2017.107798
Authors
Saman Sadeghvaziri1; Borhan Shokrollahi* 2; Sabah Mohammadi3
1Sadegh Vaziri, S., MSc, Poultry Physiology, Islamic Azad University Branch Sanandaj.
2Asistant Professor Islamic Azad University Branch Sanandaj.
3MSc in Poultry Physiology, University of Kurdestan.
Abstract
The aim of the present study was to immunohistochemical positioning of ghrelin in testicular and epididymis tissues of Kurdish ram. Ghrelin, a 28-amino acid acylated peptide is an important factor involved in most of the metabolic and hormonal signals which adapt the reproductive functions in conditions of altered energy balance. This study investigated the immunohistochemical localization of ghrelin in testicular and epididymis tissues of Kurdish ram, using mouse monoclonal anti ghrelin antibody as primary antibody and polyclonal donkey anti IgG Horseradish Peroxidase (HRP) antibody as secondary antibody. Samples of testis collected from three native male sheep aged of 1.5 to 2 years old, and preserved in 10% formalin for posterior inclusion in paraffin. Histological sections with 5 micron in thickness were prepared for IHC. Immunoreactions was assessed for Germ cells, Leydig and Sertoli cells and head of epididymis. It is believed that the site of ghrelin expression in the spermatogenesis process, Leydig, sertoli cells and head of epididymis may indicate its role in local (autocrine / paracrine) regulations. This is one of the first studies to provide molecular evidence for the presence of ghrelin within the entire testicular and epididymis tissues of ram.
Keywords
Ghrelin; leydig; sertoli; epididymis; kurdish ram
References

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