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The Study of Booroola Gene Polymorphism In Zandi Breed Sheep by PCR-RFLP | ||
| علوم و فنون دامپروری | ||
| Article 1, Volume 5, Issue 19, September 2016, Pages 3-8 PDF (764.99 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/aasrj.2016.107582 | ||
| Authors | ||
| Lida Taher Khani; Alireza Noshary* ; alireza Noshary | ||
| Islamic Azad University, Karaj Branch | ||
| Abstract | ||
| The high ovulation rate and litter size were most important factors on fecundity and could be effect on more economic prolificacy in sheep breeding industries. The aim of this study was investigation of genetic polymorphism in Booroola gene region in Zandi breed sheep. For this a sample of 170 Zandi breed sheep were selected in Ram breeding farms in Ghom province. The genomic DNA was extracted by salting out method. The PCR was done for amplify of 190 bp of FecB gene in Exon 8 available in NCBI and the PBR reaction was done by AvaII restriction endonuclease (RE). The RFLP products were loaded on Agars and used for genotyping by comparison with suitable size marker. The results showed that there is A allele in all samples only and we can’t find any mutation in AvaII RE site in studied sample. So, the studied locus of FecB was monomorph. Despite of twining ability of Zandi breed sheep we find there was no association between mutation in Exon 8 of Booroola gene and twining ability in Zandi breed sheep and the study of other region of FecB or other major genes can be purposed. | ||
| Keywords | ||
| Zandi; Booroola; Prolificacy; Polymorphism; PCR-RFLP | ||
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