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Subtyping of Salmonella enterica isolated from humans and food animals using Pulsed-Field Gel Electrophoresis | ||
| Archives of Razi Institute | ||
| مقاله 4، دوره 71، شماره 2، شهریور 2016، صفحه 97-102 اصل مقاله (230.92 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22034/ari.2000.106447 | ||
| نویسندگان | ||
| N. Golab1؛ N. Khaki* 2؛ F. Noorbakhsh1 | ||
| 1Department of Microbiology, Faculty of Science, Islamin Azad University, Varamin Branch, Varamin, Iran | ||
| 2Department of Microbiology, Razi Vaccine & Serum Research Institute, Karaj, Iran | ||
| چکیده | ||
| Salmonella infections are the second leading cause of zoonotic bacterial foodborne illness. Main source of infection in human is contaminated food products. The aim of this study was sub typing isolates of Salmonella entericaobtained during our previous study byPulsed Field Gel Electrophoresis (PFGE) technique. All 46 Salmonella isolates were serotyped and then subjected to PFGE. Total isolates were analyzed by means of the molecular technique XbaI PFGE. In this study, PFGE and serotyping were used to subtype 46 Salmonella isolates belonging to 27different serovars and derived from human and different food origins. Among these isolates, S. Typhimurium was found to be the most predominant serovar. 40 PFGE patterns out of 46 isolates were obtained. The Discrimination Index obtained by serotyping (DI = 0.93) was lower than PFGE (DI = 0.99). Subtyping of Salmonella enterica is very important and shows that animal origin can be one of a reservoir that potentially could be transferred to human through the food chain. In addition, results of this study also revealed that this procedure is a golden standard for genotyping of such salmonellaserotypes. | ||
| کلیدواژهها | ||
| PFGE؛ Salmonella enterica؛ serotyping؛ Subtyping؛ Human؛ Food animals | ||
| عنوان مقاله [English] | ||
| شناسایی زیرگونه سالمونلا انتریکا، مطالعه جدا شده از انسان و خوراک دام با استفاده از Pulse-Field الکتروفورز | ||
| نویسندگان [English] | ||
| نرگس گلاب1؛ پژواک خاکی2؛ فاطمه نوربخش1 | ||
| 1گروه میکروب شناسی، دانشکده علوم، دانشگاه آزاد اسلامی واحد ورامین، ورامین ایران | ||
| 2بخش میکروب شناسی، موسسه تحقیقات واکسن و سرم سازی رازی، کرج، ایران | ||
| چکیده [English] | ||
| آلودگی سالمونلا دومین علت بیماریهای مشترک بین انسان و دام می باشد که با منشا مواد خوراکی قابل سرایت است. منبع اصلی این عفونت در انسان محصولات غذایی آلوده میباشد. هدف از انجام این مطالعه، جداسازی زیرگونه سالمونلا انتریکا میباشد که در مطالعه پیشین توسط تیم تحقیقاتی حاضر و با استفاده از روش الکتروفورز پالس فیلد صورت گرفته است. همه 46 گونه جدایه سالمونلا تعیین سروتیپ شده و سپس مورد آزمون PFGE قرار گرفتند. همه جدایهها با استفاده از روش های مولکولی XbaI PFGE بررسی شدند. در این مطالعه PFGE و سروتایپینگ برای جداسازی 46 زیرگونه سالمونلا که متعلق به 27 سرووار مختلف میباشد، مورد استفاده قرار گرفت. این زیرگونهها با منشاء انسان و منابع غذایی مختلف به دست آمدهاند. در میان این جدایه ها سالمونلا تیفیموریوم به عنوان شایعترین سرووار شناسایی شد. از 46 جدایه، 40 الگوی PFGE بدست آمده است. شاخص تمایز بدست آمده ناشی از سروتایپینگ (DI=0.93) پایینتر از شاخص مربوط به PFGE (DI=0.99) بوده است. تعیین زیرگونه سالمونلا بسیار مهم بوده و نشان دهنده منشا حیوانی است که به عنوان یکی از منابع ذخیرهای این آلودگی قلمداد شده و بالقوه دارای توانایی انتقال به انسان از طریق زنجیره غذایی میباشد. ضمنا نتایج این مطالعه مشخص نمود که این رویه به عنوان یک استاندارد طلایی به منظور تعیین ژنوتیپ سروتیپهای مختلف سالمونلا به حساب میآید. | ||
| کلیدواژهها [English] | ||
| PFGE, سالمونلا انتریکا, سروتایپینگ, تعیین زیرگونه, انسان, دام | ||
| مراجع | ||
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Archambault, M., Petrov, P., Hendriksen, R.S., Asseva, G., Bangtrakulnonth, A., Hasman, H., Aarestrup, F.M., 2006. Molecular characterization and occurrence of extended-spectrum -lactamase resistance genes among Salmonella enterica serovar Corvallis from Thailand, Bulgaria, and Denmark. Microb Drug Resist 12. Fendri, I., Ben Hassena, A., Grosset, N., Barkallah, M., Khannous, L., Chuat, V., Gautier, M., Gdoura, R., 2013. Genetic diversity of food-isolated Salmonella strains through Pulsed Field Gel Electrophoresis (PFGE) and Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR). PLoS One 8, e81315. Fitzgerald, C., Helsel, L.O., Nicholson, M.A., Olsen, S.J., Swerdlow, D.L., Flahart, R., Sexton, J., Fields, P.I., 2001. Evaluation of methods for subtyping Campylobacter jejuni during an outbreak involving a food handler. J Clin Microbiol 39, 2386-2390. Foley, S.L., Lynne, A.M., 2008. Food animal-associated Salmonella challenges: pathogenicity and antimicrobial resistance. J Anim Sci 86, E173-187. Foley, S.L., Lynne, A.M., Nayak, R., 2009. Molecular typing methodologies for microbial source tracking and epidemiological investigations of Gram-negative bacterial foodborne pathogens. Infect Genet Evol 9, 430-440. Garaizar, J., Lopez-Molina, N., Laconcha, I., Lau Baggesen, D., Rementeria, A., Vivanco, A., Audicana, A., Perales, I., 2000. Suitability of PCR fingerprinting, infrequent-restriction-site PCR, and pulsed-field gel electrophoresis, combined with computerized gel analysis, in library typing of Salmonella enterica serovar enteritidis. Appl Environ Microbiol 66, 5273-5281. Gaul, S.B., Wedel, S., Erdman, M.M., Harris, D.L., Harris, I.T., Ferris, K.E., Hoffman, L., 2007. Use of pulsed-field gel electrophoresis of conserved XbaI fragments for identification of swine Salmonella serotypes. J Clin Microbiol 45, 472-476. Gerner-Smidt, P., Kincaid, J., Kubota, K., Hise, K., Hunter, S.B., Fair, M.A., Norton, D., Woo-Ming, A., Kurzynski, T., Sotir, M.J., Head, M., Holt, K., Swaminathan, B., 2005. Molecular surveillance of shiga toxigenic Escherichia coli O157 by PulseNet USA. J Food Prot 68, 1926-1931. Gillespie, B.E., Mathew, A.G., Draughon, F.A., Jayarao, B.M., Oliveri, S.P., 2003. Detection of Salmonella enterica somatic groups C1 and E1 by PCR-enzyme-linked immunosorbent assay. J Food Prot 66, 2367-2370. Golab, N., Khaki, P., Noorbakhsh, F., 2014. Molecular Typing of Salmonella Isolates in Poultry by Pulsed-Field Gel Electrophoresis in Iran. Int J Enteric Pathog 2, e21485. Gorman, R., Adley, C.C., 2004. Characterization of Salmonella enterica serotype Typhimurium isolates from human, food, and animal sources in the Republic of Ireland. J Clin Microbiol 42, 2314-2316. Harbottle, H., White, D.G., McDermott, P.F., Walker, R.D., Zhao, S., 2006. Comparison of multilocus sequence typing, pulsed-field gel electrophoresis, and antimicrobial susceptibility typing for characterization of Salmonella enterica serotype Newport isolates. J Clin Microbiol 44, 2449-2457. Healy, M., Huong, J., Bittner, T., Lising, M., Frye, S., Raza, S., Schrock, R., Manry, J., Renwick, A., Nieto, R., Woods, C., Versalovic, J., Lupski, J.R., 2005. Microbial DNA typing by automated repetitive-sequence-based PCR. J Clin Microbiol 43, 199-207. Herikstad, H., Motarjemi, Y., Tauxe, R.V., 2002. Salmonella surveillance: a global survey of public health serotyping. Epidemiol Infect 129, 1-8. Hunter, P.R., Gaston, M.A., 1988. Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. J Clin Microbiol 26, 2465-2466. Kumao, T., Ba-Thein, W., Hayashi, H., 2002. Molecular subtyping methods for detection of Salmonella enterica serovar Oranienburg outbreaks. J Clin Microbiol 40, 2057-2061. Liebana, E., Guns, D., Garcia-Migura, L., Woodward, M.J., Clifton-Hadley, F.A., Davies, R.H., 2001. Molecular typing of Salmonella serotypes prevalent in animals in England: assessment of methodology. J Clin Microbiol 39, 3609-3616. Logue, C.M., Nolan, L.K., 2009. Molecular Analysis of Pathogenic Bacteria and Their Toxins. In: Toldrá, F. (Ed.), Safety of Meat and Processed Meat, Springer New York, New York, NY, pp. 461-498. Majowicz, S.E., Musto, J., Scallan, E., Angulo, F.J., Kirk, M., O'Brien, S.J., Jones, T.F., Fazil, A., Hoekstra, R.M., International Collaboration on Enteric Disease 'Burden of Illness, S., 2010. The global burden of nontyphoidal Salmonella gastroenteritis. Clin Infect Dis 50, 882-889. Modarressi, S., Thong, K., 2010. Isolation and molecular sub typing of Salmonella enterica from chicken, beef and street foods in Malaysia. Sci Rese Essay 5, 2713-2720. Olsen, J.E., Skov, M.N., Threlfall, E.J., Brown, D.J., 1994. Clonal lines of Salmonella enterica serotype Enteritidis documented by IS200-, ribo-, pulsed-field gel electrophoresis and RFLP typing. J Med Microbiol 40, 15-22. Popoff , M.Y., Bockemuhl, J., Gheesling. L, L., 2004. To the Kauffmann-White scheme. Res Microbiol 155, 568-570. Rahmani, M., Peighambari, S.M., Svendsen, C.A., Cavaco, L.M., Agersø, Y., Hendriksen, R.S., 2013. Molecular clonality and antimicrobial resistance in Salmonella enterica serovars Enteritidis and Infantis from broilers in three Northern regions of Iran. BMC Veterinary Research 9, 1-9. Sandt, C.H., Fedorka-Cray, P.J., Tewari, D., Ostroff, S., Joyce, K., M'Ikanatha N, M., 2013. A comparison of non-typhoidal Salmonella from humans and food animals using pulsed-field gel electrophoresis and antimicrobial susceptibility patterns. PLoS One 8, e77836. Stepan, R.M., Sherwood, J.S., Petermann, S.R., Logue, C.M., 2011. Molecular and comparative analysis of Salmonella enterica Senftenberg from humans and animals using PFGE, MLST and NARMS. BMC Microbiol 11, 153. Wattiau, P., Boland, C., Bertrand, S., 2011. Methodologies for Salmonella enterica subsp. enterica Subtyping: Gold Standards and Alternatives. Applied and Environmental Microbiology 77, 7877-7885. Woo, Y.K., 2005. Finding the sources of Korean Salmonella enterica serovar Enteritidis PT 4 isolates by pulsed-field gel electrophoresis. J Microbiol 43, 424-429. Zahraei- Salehi, T., Madadgar, O., Tadjbakhsh, H., Mahzounieh, M.R., Feizabadi, M.M., 2011. Molecular study of the Salmonella enterica serovars Abortusovis, Typhimurium, and Enteritidis. Turk J Vet Anim Sci 35, 281-294. Zou, W., Lin, W.J., Foley, S.L., Chen, C.H., Nayak, R., Chen, J.J., 2010. Evaluation of pulsed-field gel electrophoresis profiles for identification of Salmonella serotypes. J Clin Microbiol 48, 3122-3126. | ||
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