| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شمارهها | 3,196 |
| تعداد مقالات | 35,423 |
| تعداد مشاهده مقاله | 52,253,861 |
| تعداد دریافت فایل اصل مقاله | 91,783,998 |
MOLECULAR PHYLOGENY OF ROCHELIA (BORAGINACEAE) BASED ON NRDNA ITS AND CPDNA TRNL-F SEQUENCES | ||
| The Iranian Journal of Botany | ||
| مقاله 3، دوره 16، شماره 1، شهریور 2010، صفحه 22-29 اصل مقاله (446.69 K) | ||
| نوع مقاله: Research Paper | ||
| نویسندگان | ||
| Maryam Khoshsokhan1؛ Shahrokh Kazempour Osaloo* 2؛ Sara Saadatmand3؛ Farideh Attar4 | ||
| 1Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Qom Branch, Qom, Iran | ||
| 2Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran | ||
| 3Department of Plant Biology, Faculty of Basic Sciences, Islamic Azad University, Science & Research Branch, Tehran, Iran | ||
| 4Department of Plant Biology, School of Biology, University of Tehran, Tehran, Iran | ||
| چکیده | ||
| We here present molecular phylogeny of the genus Rochelia (Boraginaceae-Eritrichieae). A total of 8 species of Rochelia and 2 species of Lappula as outgroups were included in analyses using nrDNA ITS and cpDNA trnL-F separately and in combination. To examine evolutionary trend of morphological characters, we mapped six diagnostic characters on the combined tree using MacClade 4. The analyses revealed that sect. Rochelia due to inclusion of the monotypic section Cryptocarpa (Rochelia cardiosepala) is not monophyletic. Likewise, its subsections, Rochelia and Pedunculares are paraphyletic. Rochelia persica and R. disperma along with R. cancellata of the monospecific subgenus Neo-Rochelia, as unresolved branches, were sisters to the remaining species. One of six diagnostic characters examined (non-hamate tip of calyx hairs) had evolved as reversal inboth R. persica and R. bungei and the other one (nutlets completely clasping the adaxial part of gynobase) had undergone parallel evolution between R. cancellata plus R. peduncularis and R. cardiosepala. Based on the present molecular analyses, the current infrageneric classification of Rochelia, at least at the sectional and subsectional level based upon traditional morphological characters is artificial. | ||
| کلیدواژهها | ||
| Boraginaceae؛ cpDNA trnL-F؛ Eritrichieae؛ Molecular Phylogeny؛ nrDNA ITS؛ Rochelia | ||
| عنوان مقاله [English] | ||
| فیلوژنی مولکولی جنس ( Rochelia (Boraginaceae بر اساس توالی های nrDNA ITS و cpDNA trnL-F | ||
| نویسندگان [English] | ||
| مریم خوشسخن مظفر1؛ شاهرخ کاظم پور اوصالو2؛ سارا سعادتمند3؛ فریده عطار4 | ||
| 1استادیار، گروه زیست شناسی دانشگاه آزاد اسلامی، واحد قم | ||
| 2دانشیار، گروه علوم گیاهی، دانشگاه تربیت مدرس | ||
| 3استادیار، گروه زیست شناسی دانشگاه آزاد اسلامی، واحد علوم و تحقیقات | ||
| 4دانشیار، گروه زیست شناسی گیاهی، دانشگاه تهران | ||
| چکیده [English] | ||
| تعداد 8 گونه از جنس Rochelia و دو گونه از Lappula (به عنوان برون گروه) با استفاده از توالی هستهای ITS و توالی کلروپلاستی trnL-F به طور جداگانه و نیز به صورت ترکیبی آنالیز شدند. برای مشخص کردن روند تکامل صفات مورفولوژیکی، شش صفت تشخیصی بر روی درخت ترکیبی با استفاده از برنامه MacClade 4 نشان داده شد. آنالیز ها آشکار کرد که بخشه Rochelia به دلیل قرار گرفتن بخشه مونوتیپیک Cryptocarpa (Rochelia cardiosepala) در میان آن، تک تبار نیست. همچنین زیر بخشه های مربوط به این بخشه (Rochelia وPedunculares) تک تبار نیستند.Rochelia persica و R. dispermaهمراه با R. cancellataاز زیرجنس تک گونه ای Neo- Rochelia، در شاخههایی با روابط حل نشده، به عنوان گروه خواهری با بقیه گونهها قرار می گیرند. یکی از شش صفت تشخیصی (کرک های کاسه با راس غیر قلابی) به عنوان یک صفت برگشت در هر دوگونه ی R. persicaو R. bungeiمشاهده شد و صفت دیگر (فندقهها به طور کامل متصل به بخش شکمی ژینوباز) متحمل تکامل موازی در بین سه گونه Rochelia cancellata، R. peduncularisو R. cardiosepala شده بود. بر اساس آنالیز مولکولی حاضر، رده بندی موجود در سطوح تحت جنس در Rochelia، حداقل در سطح بخشه و زیر بخشه با استفاده از صفات مورفولوژیکی، مصنوعی است. | ||
| کلیدواژهها [English] | ||
| Boraginaceae, cpDNA trnL-F, Eritrichieae, Molecular Phylogeny, nrDNA ITS, Rochelia | ||
| مراجع | ||
|
Ahangarian, S., Kazempour Osaloo, S. & Maassoumi, A. A. 2007: Molecular phylogeny of the tribe Hedysareae with special reference to Onobrychis (Fabaceae) as inferred from nrDNAITS sequences. -Iran. Journ. Bot. 13: 64- 74. Baldwin, B. G. 1992: Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: An example from the Compositae. -Mol. Phylogenet. Evol. 1: 3-16. Baldwin, B. G., Sanderson, M. J., Porter, J. M., Wojciechowski, M. F., Campbell, C. S. & Donoghue, M. J. 1995: The ITS region of nuclear ribosomal DNA: A valuable source of evidence on angiosperm phylogeny. - Ann. Mo. Bot. Gard. 82: 247- 277. De Candolle, A. P.1846: Rochelieae in Prodromnus Systematis Naturalis Regni Vegetabilis 10: 175-176. -Sumptibus Victoris Masson, Parisii. Diez, M. J. & Benito, V. 1991: Pollen morphology of the tribes Eritrichieae and Cynoglosseae (Boraginaceae) in the Iberian Peninsula and its taxonomic significance. -Bot. Journ. Linn. Soci. 107: 49- 66. Doyle, J. J. & Doyle, J. L. 1987: A rapid DNA isolation of fresh leaf tissue. –Phytochem. Bull. 19:11-15. Farris, J. S., Kallersjo, M., Kluge, A. G. & Bult, C. 1995: Testing significance of incongruence. -Cladistics. 10:315-319. Felsenstein, J.1985: Confidence limits on phylogenies: An approach using the bootstrap. -Evolution. 39: 783- 791. Hall, T. A. 1999: BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows95/98/NT. -Nucl. Acids. Symp. Ser. 41: 95-98. Hilger, H. H.1984: Gynoecium and fruit development in Rochelia disperma (Boraginaceae) and the arrangement of Rochelia mericarps. -Pl. Syst. Evol. 146: 123- 139. Holmgren, P. K. & Holmgren, N. H.1998: [continuously updated]. Index Herbariorum: A global directory of public herbaria and associated staff. -New York Botanical Garden's Virtual Herbarium. -http://sweetgum.nybg.org/ih/. Kazempour Osaloo, S.1993: Revision of the tribe Eritrichieae (Boraginaceae) in Iran, M.S. thesis (unpubl.). -Tarbiat Modares University of Tehran. Kazempour Osaloo, S., Maassoumi, A. A. & Murakami, N. 2003: Molecular systematics of the genus Astragalus L. (Fabaceae): Phylogenetic analyses of nuclear ribosomal DNA internal transcribed spacers and chloroplast gene ndhF sequences. -Plant Syst. Evol. 242: 1-32. Kazempour Osaloo, S., Maassoumi, A. A. & Murakami, N.2005: Molecular systematics of the Old World Astragalus (Fabaceae) as inferred from nrDNA ITS sequence data. -Brittonia. 57: 367-381. Khatamsaz, M. 2001: Pollen morphology of Iranian Boraginaceae family and its taxonomic significance. -Iran. Journ. Bot. 9 (1): 27-40. Khatamsaz, M.2002: Boraginaceae in Assadi & al. Flora of Iran no. 39. -Research Institute of Forests and Rangelands. Tehran. Khoshsokhan, M. & Kazempour Osaloo, S. 2008: Phylogenetic analysis of tribe Eritrichieae (Boraginaceae) based on cpDNA (trnL intron/trnL-trnF intergenic spacer) sequences . -15th national and 3rd international conference of biology, 37 (abstract). Khoshsokhan, M., Kazempour Osaloo, S., Attar, F., Saadatmand, S. & Nejadsattari, T.2008: Phylogeny of tribe Eritrichieae (Boraginaceae) based on nrDNA ITS. -10th congressIranian Genetics Society, 273 (abstract). Larkin, M. A., Blackshields, G., Brown, N. P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I. M., Wilm, A., Lopez, R., Thompson, J. D., Gibson, T. J. & Higgins, D. G. 2007: Clustal W and Clustal X version 2.0. -Bioinformatics 23: 2947-2948. Luque, T.1992: Karyological studies on Spanish Boraginaceae. VI. Contribution to the tribe Eritrichieae. –Bot. Journ. Linn. Soc. 110: 77-94. Mabberley, D. J.1990: The plant book: a portable dictionary of the higher plants. -Cambridge University Press. Cambridge. Maddison, W. P. & Maddison, D. R. 2005: MacClade: Analysis of Phylogeny and Character Evolution. Version 4.08. Sinauer Association. -Sunderland, Massachusetts. Nasir, Y. J.1989: Boraginaceae in S. I. Ali and Y. J. Nasir Flora of Pakistan no. 191. -Pakistan Agricultural Research Council, Islamabad. Popov, M. G.1953: Boraginaceae in B. K. Shischkin Flora USSR, vol. 19: 97-691. -Moskva & Leningrad. Riedl, H.1967: Boraginaceae in K. H. Rechinger Flora Iranica no. 48. -Akademische Druck, Graz, Austria. Sang, T., Crawford, D. J. & Stuessy, T.1995: Documentation of reticulate evolution in peonies (Paeonia) using internal transcribed spacer sequences of nuclear ribosomal DNA: Implications for biogeography and concerted evolution. -Proc. Natl. Acad. Sci. USA. 92: 6813-6817. Shaw, J., Lickey, E. B., Beck, J. T., Farmer, S. B., Liu, W., Miller, J., Siripun, K. C., Winder C. T., Schilling, E. E. & Small R. L. 2005: The tortoise and the hare II: Relative utility of 21 noncoding chloroplast DNA sequences for phylogenetic analysis. –Amer. Journ. Bot. 921: 142- 166. Swofford, D. L.2002: Phylogenetic analysis using parsimony (PAUP). Ver. 4. Sinauer Associates, Sunderland, Massachusetts. Taberlet, P., Gielly, L., Pautou, G. & Bouvet, J.1991: Universal primers for amplification of three non-coding regions of chloroplast DNA.- Plant Molec. Biol. 17: 1105- 1109. White, T. J., Bruns, T., Lee, S. & Taylor, J. 1990: Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics in Innis DH. Gelfand JJ. Sninsky et al. (eds.) PCR protocols: a guide to methods and applications. -Academic Press, San Diego. pp. 315-322. | ||
|
آمار تعداد مشاهده مقاله: 1,634 تعداد دریافت فایل اصل مقاله: 1,517 |
||