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بررسی اثر ضدویروسی عصاره آبی گیاه سرخاب (Phytolacca americana L.) در کنترل ویروس وای سیبزمینی | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 10، دوره 31، شماره 3 - شماره پیاپی 71، مرداد 1394، صفحه 479-488 اصل مقاله (509.11 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2015.101898 | ||
| نویسندگان | ||
| اطهر علیشیری* 1؛ فرشاد رخشندهرو2 | ||
| 1دانشجوی کارشناسی ارشد رشته بیماریشناسی گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران | ||
| 2استاد، گروه بیماریشناسی گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران | ||
| چکیده | ||
| سرخاب یا علفپوک (pokeweed) (Phytolacca americana L.) گیاه بومی کشورهای مختلف قاره آمریکا بوده و با وجود احتمال سمّیت گوارشی، از توان کنترلکنندگی برخی عوامل بیماریزای انسانی و گیاهی برخوردار است. این ویژگی بهدلیل حضور پروتئین غیرفعالکننده ریبوزوم (RIPs) در این گیاه میباشد. در این تحقیق، اثر عصاره آبی برگ و ساقه گیاه سرخاب در کنترل ویروس وای سیبزمینی (Potato virus Y-PVY) که از مهمترین ویروسهای آلودهکننده سیبزمینی است، در گیاه مدل توتون مورد بررسی قرار گرفت و حضور پروتئین ضدویروسی گیاه سرخاب (PAP) در عصاره با استفاده از SDS-PAGE تأیید شد. نتایج آزمون سرولوژیکی الایزا (DAS-ELISA) حکایت از کاهش میانگین جذب سرولوژیکی چاهکهای مربوط به نمونههای تیمار شده با عصاره آبی سرخاب در مقایسه با چاهک شاهد (نمونههای آلوده به ویروس که با عصاره علف پوک تیمار نشدهاند) در آزمون الایزا داشت. نتایج نشان داد که عصاره آبی گیاه سرخاب در غلظت ppm14/0 قادر است میزان آلودگی ویروس وای سیبزمینی را در گیاهان آلوده کاهش دهد. بهطوری که بیشترین میزان کنترلکنندگی عصاره تا چهار روز بعد از تیمار توتونهای آلوده به ویروس با عصاره سرخاب مشاهده شد. | ||
| کلیدواژهها | ||
| گیاه سرخاب (Phytolacca americana L.)؛ عصاره آبی؛ اثر ضدویروسی؛ ویروس وای سیبزمینی | ||
| عنوان مقاله [English] | ||
| Study on the antiviral activity of aqueous pokeweed (Phytolacca Americana L.) extracts against Potato virus Y | ||
| نویسندگان [English] | ||
| A. Alishiri1؛ F. Rakhshandehroo2 | ||
| 1Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| چکیده [English] | ||
| Pokeweed (Phytolacca Americana L.) is a plant native to American countries which is widely used across the continent. Despite the probability of having digestive toxicity, this plant has an important role in many therapeutic applications for humans and plants. This plant has a capacity to control some plant and human pathogens due to the presence of ribosome-inactivating proteins (RIPs) in this plant. In this work, the antiviral effect of aqueous pokeweed leaf and stem aqueous extracts against Potato virus Y, one of the most important viruses infecting potatoes, was studied in tobacco model plants. The presence of the pokeweed antiviral protein (PAP) in the extract was confirmed by SDS-PAGE analysis. Results of serological assay (DAS-ELISA) showed lower absorbance values for the pokeweed treated samples as compared with non-treated, PVY-infected control plants, indicating lower levels of PVY infections in the treated plants. Results showed that the aqueous extract of pokeweed, at concentration of 0.14 ppm, had an inhibitory effect against PVY infection in tobacco model plants with a maximum controlling effect up to four days post-inoculation. | ||
| کلیدواژهها [English] | ||
| Pokeweed (Phytolacca americana L.), aqueous extract, antiviral activity, Potato virus Y | ||
| مراجع | ||
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