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تاثیر سیلوکردن محصول فرعی پسته بر فعالیت‌های تخمیری و کنترل تولید متان در شکمبه در شرایط آزمایشگاهی | ||
| علوم دامی | ||
| مقاله 4، دوره 28، شماره 106، خرداد 1394، صفحه 43-54 اصل مقاله (1.01 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2015.101346 | ||
| نویسندگان | ||
| پیروز شاکری* 1؛ علی‌رضا آقاشاهی2؛ حمید مصطفوی3؛ مهدی میرزایی4 | ||
| 1بخش تحقیقات علوم دامی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان کرمان، سازمان تحقیقات، آموزش و ترویج کشاورزی. | ||
| 2موسسه تحقیقات علوم دامی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی | ||
| 3مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان کرمان، سازمان تحقیقات، آموزش و ترویج کشاورزی | ||
| 4گروه علوم دامی، دانشکده کشاورزی و منابع طبیعی، دانشگاه اراک | ||
| چکیده | ||
| این آزمایش با هدف بررسی تاثیر تانن موجود در محصول فرعی پسته آفتاب‌خشک و سیلو‌شده بر فعالیت‌های تخمیری و کنترل تولید متان در شکمبه در شرایط آزمایشگاهی انجام شد. به‌این منظور، محصول فرعی پسته بدون افزودنی در یک سیلوی خندقی سیلو شد و هم‌زمان بخشی از آن در مقابل آفتاب خشک گردید. پس‌از 3 ماه نمونه‌های سیلاژ با روش خشک کردن انجمادی خشک شد. سپس ترکیبات شیمیایی هر دو نوع محصول تعیین شد. تخمیرپذیری نمونه‌ها با 4 تکرار از طریق انکوباسیون به‌همراه مایع شکمبه بافری شده و به‌مدت 24 ساعت در شرایط آزمایشگاهی تعیین شد. از قصیل جو و یک کنسانتره تجاری به‌عنوان کنترل مثبت استفاده گردید. در پایان حجم‌گاز، متان تولیدی، غلظت کل و نسبت هر یک از اسیدهای چرب فرار و غلظت آمونیاک تعیین شد. سیلوکردن محصول فرعی پسته، افزایش ترکیبات فنلی و کاهش کربوهیدرات‌های محلول در آب و الیاف نامحلول در شوینده خنثی را به‌همراه داشت (05/0>P). حجم‌گاز و آمونیاک تولیدی هر دو نوع محصول فرعی پسته به‌طور معنی‌داری از قصیل جو و کنسانتره تجاری کمتر بود (01/0>P)، همچنین مقدار کل اسیدهای چرب فرار در هر دو نوع محصول از کنسانتره تجاری کمتر (01/0>P) بود. کمترین نسبت استات به پروپیونات و بالاترین pH با تخمیر سیلاژ محصول فرعی پسته مشاهده شد (05/0>P) و در اثر تخمیر هر دو نوع محصول فرعی، غلظت کمتری (01/0>P) از متان در مقایسه با نمونه‌های کنترل مثبت تولید شد. به‌طور کلی نتایج نشان داد که محصول فرعی پسته باعث کاهش تولید متان گردید و می‌تواند به‌عنوان راهکاری جدید برای دستکاری تخمیر شکمبه‌ای استفاده شود | ||
| کلیدواژه‌ها | ||
| محصول فرعی پسته؛ تانن؛ متان؛ اسیدهای چرب فرار و آمونیاک | ||
| عنوان مقاله [English] | ||
| Effects of ensiling pistachio by-products on ruminal fermentation and methane emission mitigation using in vitro batch fermentation | ||
| نویسندگان [English] | ||
| Pirouz shakeri1؛ Ali reza Aghashahi2؛ Hamid Mostafavi3؛ Mehdi Mirzaee4 | ||
| چکیده [English] | ||
| The present study was conducted to examine the effects of sun dried and ensiled pistachio by-product (PBP) on fermentation and methane emission in the rumen using an in vitro batch fermentation system. For this purpose, fresh PBP were ensiled into a trench silo without any additives and simultaneously some sun dried PBP were prepared. After 3 month, the ensiled PBP samples were freeze-dried and then chemical analysis was conducted for both samples. All samples were incubated with four replications using buffered rumen fluid for 24 hours. Two positive controls (i.e., oaten chaff and commercial concentrate) were included in the assay as standards to detect differences. At the end of incubation gas pressure, pH and total volatile fatty acids were used as indicators of overall sample fermentability, and concentrations of methane, ammonia, acetate and propionate indicated changes in fermentation end products. The result showed that the ensiled PBP had lower aNDFom and water soluble carbohydrates but greater in phenolic compounds compared with the sun dried PBP. Gas production were lower (P<0.01) in sun dried and ensiled pistachio by-products than the oaten chaff and commercial concentrate, although the amounts of volatile fatty acids and ammonia for both PBPwere lower (P<0.01) than the commercial concentrate. The lowest acetate to propionate ratio and the highest pH were in ensiled PBPhowever, a significant reduction (P<0.01) in methane emission was observed with both PBP samples in comparison to the positive controls. In general, these findings indicate that PBP has a great potential to decrease methane mitigation and can used as a new alternative for modify of rumen fermentation | ||
| کلیدواژه‌ها [English] | ||
| Pistachio by-products, tannin, methane, volatile fatty acids, ammonia | ||
| مراجع | ||
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