Comparison of several treatment methods against saprolegniosis in rainbow trout fertilized eggs

Akhlaghi, A; Bahaoodini, A.A

doi:10.22092/vj.2012.101075

	Comparison of several treatment methods against saprolegniosis in rainbow trout fertilized eggs
تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Editorial, Volume 25, Issue 1, March 2012, Pages 18-24 PDF (497.55 K)
DOI: 10.22092/vj.2012.101075
Authors
A Akhlaghi^* ¹; A.A Bahaoodini²
¹School of Veterinary Medicine, Shiraz University
²School of Veterinary Medicine, Islamic Azad University, Kazerun Branch
Abstract
In this research Psedomonas fluorescens as a probiotic, sodium chloride, plant extract (Terminalia catappa), malachite green and formalin as antifungal agents were used daily for 19 days on rainbow trout fertilized eggs in order to compare their survival with untreated fertilized eggs in control group. Results showed that the percent of live eggs (eyed) after 19 days treatment with P. fluorescens (107 bacteria/ml), sodium chloride (20000 ppm), the plant extract (5 ppm), malachite green (66.7 ppm), formalin (200 ppm) and the control group were 81.3%, 86.7%, 82.9%,83.4%, 89.2%, and 77.2% respectively. Thus P. fluorescens as a probiotic, the plant extract and the other anti-fungal agents revealed significant effect in rainbow trout fertilized eggs survival anti-fungal activity as compared with the control group (P<0.05). The plant extracted antifungal agent and also the probiotic as a new agent used in this study compete with the commonly used antifungal in that these are environmentally friendly non-hazardous agents.

References
1- یعقوبی، آ، یوسفی، ج، مهرابی، م. (1380) مهار رشد ساپروگلینا دیکلینا نوع 1 جدا شده از تخم ماهیان قزل آلا با استفاده از باکتری fluroscence P. در شرایط آزمایشگاهی. دوازدهمین گردهمایی جامعه دامپزشکی ایران. تهران 15-12 اسفند . ص 234.## 2- شریف روحانی، م و همکاران. (1385) ارزیابی کاربرد اسانس شمعدانی (Geranium herbarum) در کنترل آلودگی های قارچی تخم ماهی قزل الای رنگین کمان. مجله دانشکده دامپزشکی دانشگاه تهران. دوره 61. شماره 3 صفحات 272-269.## 3- Austin B and Austin. D.A (2007) Bacterial fish pathogens: diseases of farmed and wild fish. 3rd edition, Praxis Publishing, Chichester, UK.## 4- Burapadaja, S., Tuntiwechwuttikul P. and Bunchoo. A. (1994) A constituent with antibacterial action from Terminalia catappa. Thai. J. Pharm. Sci. 18: 42-46.## 5- Chitmanat, C., Tongdonmuan K. and Nunsong. W. (2003) Antimicrobial activity derived from Terminalia catappa solution against some Tilapia pathogens. The 3rd world congress on medicinal plant and aromatic plants for human welfare, 3-7 Feb 2003, Chiang mai, Thailand.## 6- Chyau, C., Tsai, S. Ko P. and Mau. J. (2002) Antioxidant properties of solvent extracts from Terminalia catappa leaves. Food Chem. 78: 483-488.## 7- Fan, Y.M., Xu, L.Z. Gao, J. Wang, Y. Tang, X.H. Zhao X.N. and Zhang. Z.X. (2004) Phytochemical and anti-inflammatory studies on Terminalia catappa. Fitoterapia 75: 253-260.## 8- Ghiasi, M., Zahedi, A., Safari, R., Aghaei Moghadam, A.A and Binaii, M. (2010) Inhibitory effect of bacteria like Pseudomonas anguiliceptica on in vitro Saprolegnia sp. Growth. 2nd International Congress on Aquatic Animal Health Management and Diseases. October 26-27, Tehran, Iran.## 9- Gibbons, S., Ohlendorf B. and Johnsen. I. (2002) The genus Hypericum-a valuable resource of Anti-Staphylococcal leads. Fitoterapia 73: 300-304.## 10- Gurusiddaiad, S., Weller., D. M. Sarkar A. and Cook, R. J. (1986) Charachterization of an antibiotic produced by a strain 4 Pseudomonas fluorescens inhibitory to Geaumannomyces graminis var. tritici and Pythium spp. Antimicrob. Agents Chemother. 29: 488-495.## 11- Hatai, K. and Willoughby. L. G. (1988) Saprolegnia parasitica from the rainbow trout inhibited by the bacterium, pseudomonas ferax. Trans. Brit. Mycol. Soc.. 83: 257-263.## 12- Khallil, A. R. M. (2001) Phytofungitoxic properties in the aqueous extracts of some plants. Assiut J. Agri. Sci. 32: 135—143.## 13- Khodabande, S. and Abtahi. B. (2006) Effects of sodium choloride, formalin and iodine on the hatching success of common carp, Cyprinus carpio, eggs. J. Appl. Ichthyol. 22:54-56.## 14- Kim, D. H. and Austin. B. (2006) Innate immune responses in rainbow trout (Oncorhynchus mykiss, walbauns) induced by probiotics. Fish & Shellfish Immunol. 21: 513-524.## 15- Marking, L. L., Raoh J. J. and Schreier. T. M. (1994) Evaluation of antifungal agents for fish culture. Prog. fish cult. 56: 225 -231.## 16 - Ramesh Kumar, N., Thirumalai Arsu, V. and Gunasekaran, P., (2002) Genotyping of antifungal compounds producing plant growth promoting rhizobacteria, Pseudomonas fluorescence. Cur. Sci. 82: 1463-1466.## 17- Schreier, T. M., Rach, J. J. and Howe, G. E. (1996) Efficacy of formalin, hydrogen peroxide and sodium chloride on fungal infected rainbow trout eggs. Aquaculture. 140: 323-331.## 18- Shahbazian N., Ebrahimzadeh Mousavi H.A., Soltani M., Khosravi A.R., Mirzargar S. and SharifpourI. (2010) Fungal contamination in rainbow trout eggs in Kermanshah province propagations with emphasis on Saprolegniaceae. Iranian J. Fish. Sci. 9: 151-160.## 19- Waterstrat, P. R. and Marking. L.L. (1995) Clinical evaluation of formalin, hydrogen peroxide and sodium chloride for the treatment of Saprolegnia parasitica. Prog. fish Cult. 57: 287-291.## 20- Willoughby, L. G. and Roberts. R. J. (1992) Towards strategic use of fungicides against Saprolognia prasitica in salmonid fish hatcheries. J. fish Dis. 15: 1-13.## 21- Woo, P.T.R. and Bruno. D.W. (2011) Fish diseases and disorders, Vol. 3: viral bacterial and fungal infections. CAB international publication, London.##
Statistics Article View: 1,660 PDF Download: 894

Journal management system. designed by sinaweb

Related Links

Comparison of several treatment methods against saprolegniosis in rainbow trout fertilized eggs