| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شمارهها | 3,208 |
| تعداد مقالات | 35,560 |
| تعداد مشاهده مقاله | 53,003,750 |
| تعداد دریافت فایل اصل مقاله | 92,288,717 |
بررسی قدرت بیمارگری و ویژگیهای مولکولی چند جدایهی باکتری Bacillus thuringiensis روی کرم قوزهی پنبه و شبپرهی پشت الماسی | ||
| مهار زیستی در گیاهپزشکی | ||
| مقاله 2، دوره 1، شماره 2، مهر 1392، صفحه 17-26 اصل مقاله (182.24 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2013.100606 | ||
| نویسندگان | ||
| مریم کلانتری* 1؛ رسول مرزبان1؛ زهرا معقولی فرد2؛ حبیب عباسیپور2 | ||
| 1بخش تحقیقات کنترل بیولوژیک، مؤسسهی تحقیقات گیاهپزشکی | ||
| 2دانشکدهی کشاورزی دانشگاه شاهد | ||
| چکیده | ||
| باکتری Bacillus thuringiensis بهخاطر مزیتهای فراوان مانند اثر اختصاصی روی لارو حشرات آفت، عدم تأثیر نامطلوب روی محیط زیست و جاندارن غیر هدف و همچنین سهولت ترکیب و تلفیق با سایر روشهای کنترل، جایگاه ویژهای در مدیریت کنترل آفات دارد. اولین قدم بهعنوان پایه و اساس تحقیق روی این باکتری، بررسی قدرت بیمارگری جدایهها و سویههای بیمارگر درکنترل آفات مورد هدف است. در این تحقیق قدرت بیمارگری سه جدایهی بومی و یک نمونهی تجاری(Dipel®) روی لارو سن دوم کرم قوزهی پنبه،Helicoverpa armigera و شبپرهی پشت الماسی،Plutella xylostella در دمای 27 درجهی سلسیوس و رطوبت نسبی 65% و دورهی نوری 16:8 (تاریکی: روشنایی) بررسی شد. غلظت کشنده 50 درصد (LC50) سه جدایهی بومی KD2، 20، 6R و نمونهی تجاری دیپل روی لارو سن دوم کرم قوزهی پنبه بهترتیب 107×21/1، 107×8/8، 107×39/1 و 107×91/6 اسپور در میلیلیتر محاسبه شد که بیمارگری جدایهیKD-2 ، با کمترین غلظت کشنده 50 درصد روی کرم قوزهی پنبه بیشتر بود. غلظت کشندهی 50 درصد جدایههای بومی فوق روی لاروهای سن دوم شبپرهی پشت الماسی بهترتیب 104×1/6، 103×4/3، 105×9/2 و 104×8/8 اسپور در میلیلیتر بود که بیشترین قدرت بیمارگری باکتری Bt مربوط به جدایهی20 بود. در بررسی مولکولی جدایهها مشخص شد که جدایههای مورد بررسی دارای ژنهای cry یکسانی نیستند. | ||
| کلیدواژهها | ||
| Bacillus thuringiensis؛ کرم قوزهی پنبه؛ شبپرهی پشت الماسی؛ قدرت بیمارگری؛ ویژگی مولکولی | ||
| عنوان مقاله [English] | ||
| Study of virulence and molecular characteristics of some Bacillus thuringiensis isolates on cotton bollworm and diamondback moth | ||
| نویسندگان [English] | ||
| Maryam Kalantari1؛ Rasoul Marzban1؛ Zahra Magollifard2؛ Habib Abbasipour2 | ||
| چکیده [English] | ||
| Bacillus thuringiensis bacteria because of many advantages such as specific effect on pest insects larvae, no adverse effects on the environment and non-target organisms and also easy combination with other methods of pest control, has an important role in pest management programs. The first step as a basis for doing research on this bacteria is to study virulence of different isolates and strains of the pathogen in the control of the target pest. In this research, the virulence of three native isolates and a commercial product (Dipel®) was evaluated on the larvae of cotton bollworm, Helicoverpa armigera and diamondback moth, Plutella xylostella at 27oC, a relative humidity of 65% and 16L:8D photoperiod. Based on the calculated results, 50% lethal concentration (LC50) of three native isolates of KD2, 20, 6R and commercial product of Dipel on the 2nd larval instars of H. armigera were obtained as 1.21×107, 8.8×107, 1.39×107 and 6.91×107 spore ml-1, respectively and KD2 isolate was the best strain among others. Results of 50% lethal concentration (LC50) bioassay of three native isolates and commercial sample of Dipel on the 2nd larval instars of P. xylostella were calculated as 6.1×104, 3.4×103, 2. 9×105 and 8.8×104 spore ml-1, respectively and isolate of 20 was the best strain among others. The molecular analysis of the isolates revealed that isolates cry genes are not identical. | ||
| کلیدواژهها [English] | ||
| Bacillus thuringiensis, Helicoverpa armigera, Plutella xylostella, virulence, molecular characteristic | ||
| مراجع | ||
|
Behdad, A. 1982. Pests of Field Crops in Iran. Neshat publication, Isfahan. (In Persian). Ben-Dov, E., Zaritsky,A., Dahan, E., Barak, Z., Sinai, R. & Manasherob, R. 1997. Extended screening by PCR for seven cry-group genes from field collected strains of Bacillus thuringiensis. Applied Environmental Microbiology. 63: 4883–4890. Brunner, E. & Stevens, P.F.E. 1986. The control of diamondback moth with Thuricide, pp. 213-217. In: Talekar, N. S. & Griggs, T. D. (eds.), Diamondback Moth Management. Asian Vegetable Research and Development Center, Shanhua, Taiwan. Burgerjon, A. & Biache, G. 1967. Contribution a L’etude du spectre d’activite de different souches de Bacillus thuringiensis. Entomologia Experimentalis et Applicata. 10: 211-230. (In French with English summary). Deilami, A. 2010. Bioassay studies of some Bacillus thuringiensis Iranian strains on larvae of diamondback moth Plutella xylostella L. M.s. Thesis, University of Shahed, 110 pp. (In Persian). Dezianian, A., Sajap, A.S., Lau, W.H., Omar, D., Kadir, H.A., Mohzmed, R. & Yusoh, M.R.M. 2010. Morphological Characteristics of P. xylostella Granulovirus and Effects on Its Larval Host Diamondback Moth, Plutella xylostella L. (Lepidoptera: Plutellidae). American Journal of Agricultural and Biological Science. 5(1): 43-49. Eckenrode, C.J., Andaloro, J.T. & Shelton, A.M. 1981. Suppression of Lepidopterous larvae (Pieris rapae, Plutella xylostella, Trichoplusia ni) in commercial sauerkraut cabbage field and research plots (parathion, Bacillus thuringiensis). Economic Entomology. 72: 276-279. Eldridge, R. 2011. Calidad de los Productos Formulados en base a Bacillus thuringiensis. http://www.anasac.cl/agropecuarios/opensite_det_20110621205036n aspx. Evans, H.F. & Shapiro, M. 1981. Viruses, pp. 17–54. In: Lacey, L. (eds.), Manual of Techniques in Insect Pathology. Academic Press, London. Fitt, G. P. 1989. The ecology of Heliothis species in relation to agroecosystems. Annual Review of Entomology. 34: 17-52. Grzywacz, D., Rossbach, A., Rauf, A., Russell, D.A., Srinivasan, R. & Shelton, A.M. 2009. Current control methods for diamondback moth and other brassica insect pests and the prospects for improved management with lepidopteran-resistant Bt vegetable brassica in Asia and Africa. Crop Protection. 29(1): 68-79. Ho, T.H. & Ng, K.Y. 1970. Bacillus thuringiensis Berliner for the control of diamondback moth in West Malaysia. Malaysian Agriculture Journal. 47: 313-322. Hughes, P.R., Gettig, R.R. & McCarthy, W.J. 1984. Comparison of the time- Mortality response of Heliothis zea to 14 isolates of Heliothis nuclear polyhedrosis virus. Invertebrate Pathology. 41: 256-261. Izadyar, S. 2002. Bioassay of Iranian strains of Bacillus thuringiensis against Helicoverpa armigera and determination of β –exotoxin. M.s. Thesis, Reseach and Sciense of Azad University. 117 pp. (In Persian). Kumar, N.S., Murugan, K. & Zhang, W. 2008. Additive interaction of Helicoverpa armigera Nucleopolyhedrovirus and Azadirachtin. BioControl. 53: 869-880. Kurstak, E. & Tijssen, P. 1982. Mode of action, safety and future popects, pp. 3-45. In: Kurstak, E. (eds.), Microbial and Viral Pesticides. Marcel Dekki, Newyork. Levinson, B.L., Kasyan, K.J., Chiu, S.S., Currier, T.C. & Gonzalez, J.M. 1990. Identification of beta-exotoxin production, plasmids encoding beta-exotoxins, and new exotoxins in Bacillus thuringiensis by using high performance liquid chromatography. Journal of Bacteriology. 172: 3172-3179. Marzban, R. & Baniameri, V. 2004. An investigation on the effectiveness of some chemical and biological insecticides on Diamondback moth, Plutella xylostella L. (Lep.: Plutellidae). Journal of New Agricultural Science. 1: 14-20. Marzban, R. 2001. Comparative bioassay of some native isolates of Bacillus thuringiensis and serotype of kurstaki on Indian meal moth (Plodia interpunctella Hb.). Applied Entomology and Phytopathology. 70: 29-36. Marzban, R. & Salehi, J.G. 2006. Distribution of Bacillus thuringiensis in the Agricultural soils of Iran, pp. 95-100. In: Zaikov, G.E. (eds.), Biotechnology, Agriculture and the Food Industry. Nova Science Publishers, New York. Marzban, R., He, Q., Liu, X.X. & Zhang, Q.W. 2009. Effects of Bacillus thuringiensis toxin Cry1Ac and Cytoplasmic polyhedrosis virus of Helicoverpa armigera (Hübner) (HaCPV) on Cotton bollworm (Lepidoptera: Noctuidae). Journal of Invertebrate Pathology. 101: 71-76. Mitter, C., Robert, W.P. & Mattews, M. 1993. Biosystematic of the Heliothinae (Lepidoptera: Noctuidae). Annual Review of Entomology. 38: 207-225. Ooi, P.A.C. 1981. Microbial control of diamondback moth in Cameron Highlands, Malaysia. Malays Applied Biology. 10: 49-56. Rautapaa, J. 1967. Notes on the toxicity of a commercial preparation of Bacillus thuringiensis var. thuringiensis Berliner on some Lepidoptera and Tenthredinidae larvae. Annals of Agriculture Fennica. 6: 103-105. Shelton, A.M. 2004. Management of diamondback moth, pp. 47-53. In: Sivapragasam, A., Loke, W.H., Hussan, A.K. & Lim, G.S. (eds.), The Management of Diamondback Moth and other Crucifer Pests. Kualalumpur, Malaysia. Sun, C.N., Wu, T.K., Chen, J.S. & Lee, W.T. 1986. Insecticide resistance in diamondback moth, Management, PP. 359-371. Diamondback Moth. In: Talker, N.S. & Griggs, T.D. (eds.), Asian Vegetable Research and Development Center, Taiwan. Talekar, N.S. & Shelton, A.M. 1993. Biology, ecology and management of the diamondback moth.Annual Review of Entomology. 38: 275-301. Tanada, Y. & Kaya, H. K. 1993. Insect Pathology. Academic Press, Sandiego. Teakle, R.E. & Jensen, J.M. 1991. Heliothis armigera, pp. 313-332. In: Singh, p. & Moore, R.F. (eds.), Hand Book of Insect Rearing. Elsevier, Amsterdam. Travis, R.G., & O’Callaghan, M. 1989. Bacillus thuringiensis: Biology, Ecology and Safety. Wiley, New York. Verkerk, R.H.J. & Wright, D.J. 1996. Multitropic interactions and management of the diamondback moth, a review. Bulletin of Entomological Research. 86: 205-216. Wright, D. 2004. Biological control of Plutella xylostella: a global perspective, pp. 9-14. In: Bordat, D. & Kirk, A.A. (eds.), Improving Biocontrol of Plutella xylostella. Montpelier, France | ||
|
آمار تعداد مشاهده مقاله: 1,755 تعداد دریافت فایل اصل مقاله: 831 |
||