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اثر تنش خشکی بر خصوصیات فیزیولوژیکی و بیوشیمیایی گیاه سیاهدانه (Nigella sativa L.) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 10، دوره 30، شماره 4 - شماره پیاپی 66، مهر 1393، صفحه 600-610 اصل مقاله (332.31 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2014.9841 | ||
| نویسندگان | ||
| رزیتا کبیری1؛ حسن فرحبخش* 2؛ فاطمه نصیبی3 | ||
| 1دانشجوی کارشناسی ارشد، گروه زراعت، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان | ||
| 2دانشیار، گروه گیاهان دارویی، پژوهشکده باغبانی، دانشگاه شهید باهنر کرمان | ||
| 3استادیار، گروه زیستشناسی، دانشکده علوم، دانشگاه شهید باهنر کرمان | ||
| چکیده | ||
| بهمنظور بررسی اثر تنش خشکی ناشی از پلیاتیلن گلایکول بر برخی پارامترهای فیزیولوژیکی و بیوشیمیایی شامل وزن خشک اندام هوایی، نشت یونی، محتوی نسبی آب برگ، رنگیزههای فتوسنتزی (کلروفیل a، b، کل و کاروتنوئیدها)، مالوندآلدهید و سایر آلدهیدها، آنتوسیانینها، ترکیبهای پلیفنلی، فلاونوئیدها، فعالیت آنزیم فنیلآلانین آمونیالیاز، محتوی قندهای محلول و پروتئین در گیاه دارویی سیاهدانه (Nigella sativa L.)، آزمایشی در سال 1390 در آزمایشگاه تحقیقاتی دانشگاه شهید باهنر کرمان در قالب طرح کاملاً تصادفی با سه تکرار و در شرایط کشت هیدروپونیک انجام شد. تیمارهای آزمایشی شامل تنش خشکی در سطوح صفر، 2/0-، 4/0- و 6/0- مگاپاسکال بودند. نتایج نشان داد که تنش خشکی باعث کاهش معنیدار وزن خشک اندام هوایی، محتوی نسبی آب برگ، رنگیزههای فتوسنتزی، آنتوسیانینها، ترکیبهای پلیفنلی، فلاونوئیدها و پروتئین و افزایش نشت یونی، مقدار مالوندآلدهید و سایر آلدهیدها، فعالیت آنزیم فنیلآلانین آمونیالیاز و محتوی قندهای محلول گردید. براساس نتایج بدست آمده به نظر میرسد که این گیاه در برابر تنش خشکی حساس بوده، بنابراین بکار بردن ترکیبهای حفاظتی خارجی میتواند ظرفیت آنتیاکسیدانی این گیاه را در برابر شرایط تنش افزایش دهد. | ||
| کلیدواژهها | ||
| سیاهدانه (Nigella sativa L.)؛ آنتوسیانین؛ پلیفنلها؛ فلاونوئیدها؛ آنزیم فنیلآلانین آمونیالیاز | ||
| عنوان مقاله [English] | ||
| Effect of drought stress on physiological and biochemical characteristics of Nigella sativa L. | ||
| نویسندگان [English] | ||
| R. Kabiri1؛ H. Farahbakhsh2؛ F. Nasibi3 | ||
| 1MSc. Student, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran | ||
| 2Horticultural Research Institute, Shahid Bahonar University of Kerman, Kerman, Iran | ||
| 3Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran | ||
| چکیده [English] | ||
| This research was aimed to evaluate the effect of drought stress, induced by polyethylene glycol (PEG6000), on physiological and biochemical parameters of black cumin (Nigella sativa L.). The traits were included: shoot dry weight, electrolyte leakage, relative water content, photosynthetic pigments (chlorophyll a, b, total chlorophyll and carotenoids), malondialdehyde and other aldehyde's contents, anthocyanines, polyphenol compounds, flavonoids, phenylalanine ammonialayse activity, soluble sugar content and protein. An experiment was conducted as a completely randomized design with three replications under hydroponic culture at research laboratory of Shahid Bahonar University of Kerman in 2011. Treatments were four levels of drought stress (0, -0.2, -0.4 and -0.6 MPa). Results showed that drought stress caused a significant reduction in shoot dry weight, relative water content, photosynthetic pigments, anthocyanines, polyphenol compounds, flavonoids and protein and an increase in electrolyte leakage, malondialdehyde content, phenylalanine ammonialayse activity and soluble sugar. Based on the obtained results, it seems that this plant is sensitive to drought stress, and the application of exogenous protective compounds could increase the antioxidant capacity of plant against stress condition. | ||
| کلیدواژهها [English] | ||
| Nigella sativa L, Anthocyanin, polyphenol compounds, Flavonoids, phenylalanine ammonialayse | ||
| مراجع | ||
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