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مطالعه تفاوت ژنتیکی تودههای مختلف علفهرز جو دره Hordeum spontaneum با استفاده از نشانگر SSR | ||
| دانش علفهای هرز ایران | ||
| مقاله 8، دوره 12، شماره 1، شهریور 1395، صفحه 107-119 اصل مقاله (709.53 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| نویسندگان | ||
| سیروان بابایی1؛ حسن علیزاده* 2؛ محمدعلی باغستانی3؛ محمدرضا نقوی4؛ سهیلا محمدی5 | ||
| 1استادیار گروه زراعت و اصلاح نباتات دانشگاه کردستان | ||
| 2عضو هیئت علمی دانشگاه تهران | ||
| 3موسسه تحقیقات گیاهپزشکی | ||
| 4دانشگاه تهران | ||
| 5دانشجوی دکتری اصلاح نباتات دانشگاه ایلام | ||
| چکیده | ||
| بهمنظور بررسی تفاوت ژنتیکی تودههای مختلف جودره در ایران، پژوهشی طی سالهای 93-1392 در گروه زراعت و اصلاح نباتات دانشگاه تهران انجام شد. در این پژوهش 40 توده جمعآوریشده از سراسر کشور با استفاده از 11 نشانگر SSR مورد بررسی قرار گرفتند. نتایج بهطور کل نشان داد که تعداد باندهای چند شکل از 2 تا 14 باند برای هر آغازگر متغیر بود. بهطور متوسط 7 باند و 6/5 باند چند شکل برای همه آغازگرها حاصل شد. از کل واریانس مشاهده شده، در حدود 48 درصد تنوع مشاهده شده مربوط به تنوع بین جمعیتها و 52 درصد مربوط به داخل جمعیتها بود که مقدار تمایز بالای بین جمعیتها را نشان میدهد. 40 توده مورد بررسی جودره در 5 گروه مختلف قرارگرفتند. گروه الف) شامل تودههای استان فارس، گروه ب) شامل تودههای یزد، چهارمحال و بختیاری، لرستان، ایلام و یک توده همدان، گروه ج) شامل تودههای قزوین، البرز، خمین، قم و خراسان، گروه د) شامل تودههای کردستان، آذربایجان غربی، اردبیل و قزوین و گروه هـ) شامل تودههای همدان، کردستان و کرمانشاه شدند. قرارگیری تودههای مناطق نزدیک به هم در یک گروه، ممکن است به دلیل نفوذ این تودهها به داخل همدیگر، از طریق ماشین آلات کشاورزی و یا بذور گیاهان زراعی باشد. همچنین به احتمال زیاد فرضیه انتقال بذر جودره از استان فارس، حداقل به مناطق دوردست مانند کرج و ورامین مردود است. آگاهی از تنوع ژنتیکی این علفهرز، میتواند منجر به پیشرفت برنامههای مدیریتی به منظور کنترل مؤثر این علفهرز مخصوصا در مزارع گندم شود. | ||
| کلیدواژهها | ||
| پراکنش علفهرز؛ تنوع ژنتیکی؛ نشانگر SSR | ||
| عنوان مقاله [English] | ||
| Analysis of Genetic Diversity in Wild barley (Horedeum spontaneum) Populations by Using SSR Marker | ||
| نویسندگان [English] | ||
| sirvan babaei1؛ Hassan Alizadeh2؛ Mohammad ali baghestani3؛ Mohammad reza Naghavi4؛ sohila mohammadi5 | ||
| 2University of Tehran | ||
| چکیده [English] | ||
| In order to investigate genetic variation of Hordeum spontaneum C. Koch populations in Iran, an experiment was conducted at the research section of the Department of Agronomy and Plant Breeding, University of Tehran from 2013 to 2014. In this study, 40 ecotypes of H. spontaneum collected from all parts of Iran were investigated by means of 11 SSR markers. Results revealed that the number of polymorphic bands for each primer varied from 2 to 14, on average, 7 and 5.6 polymorphic bands for all primers. Total variances observed were related to diversity among and within the populations which were 48% and 52% respectively. This means a high differentiation among the populations. The biotypes of wild barely (40) were divided into 5 groups including: A: Fars populations, B: populations of Yazd, Chaharmahal va bakhtiari, Lorestan, Ilam and Hamedan populations, C: Qazvin, Alborz, Khomein, Qom and Khorasan populations, D: Kurdistan, West Azarbaijan, Lorestan, Ardebil and Qazvin populations. E: Hamedan, Kurdistan and Kermanshah populations. The placement of closed area populations in one group may be due to penetrations into each other, through farm machinery or cultivated crop seeds. The hypothesis of seed transport from Fars province to far areas such as Karaj and Varamin is rejected. Knowledge of the genetic diversity of this weed, can lead to development of management plans in order to effectively control this weed especially in wheat fields. | ||
| کلیدواژهها [English] | ||
| Genetic diversity, SSR marker, weed dispersal | ||
| مراجع | ||
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Agrama, H. and Tuinstra, M.R. 2003. Phylogenetic diversity and relationship among Sorghum accessions using SSRs and RAPDs. Afr. J. Biotechnol. 2: 334-340. Babaei, S., Alizadeh, H., Baghestani, M.A. and Naghavi, M.R. 2014. Evaluation the effect of some adjuvants on Hordeum spontaneum control in wheat at three different locations. Iranian J. Weed Sci. 14: 22-35. Baghestani, M.A., Zand,E., Moeini, M.M. and Atri, A.R. 2007. Review of the research on wield barley control in wheat fields. Proceeding of 2nd Iranian weed congress, Mashhad, Iran. 58-76. Bassam, B.J. and Gresshoff, P.M. 2007. Silver staining of DNA in polyacrylamide gels. Nat. Protoc. 2: 2649-2654. Beckie, H.J., I.M. Heap, R.J. Smeda and L.M. Hall. 2000. Screening for herbicide resistance in weeds. Weed Technol. 14: 428- 445. Brown, A.H.D., D. Zohary, and E. Nevo. 1978. Outcrossing rates and heterozygosity in natural populations of Hordeum spontaneum Koch in Israel. Heredity. 41: 49-62. El-Awady, A.M., M. El-Tarras, A.E. Adel. 2012. Genetic diversity of some Saudi barely (Hordeum vulgar) landraces based on microsatellite markers. Am. J. Appl. Sci., 752-758. Ganjkhanlou, A., Mohammadi, S.A., Moghadam,M., Ghasemi Gholezani, K., Shakiba, M.R. and Yousefi, A. 2012. Genetic diversity in barley as revealed by microsatellite markers and association analysis of these markers by traits related to freezing tolerance. Seed & Plant Impro. J. 28: 101-114. Gutterman, Y., Corbineau, F. and Come, D. 1996. Dormancy of Hordeum spontaneum caryopses from a population on the Negev Desert Highlands. J. Arid Env., 33, 337–345. Harlan, J.R., and D. Zohary. 1966. Distribution of wild wheats and barley. Science. 153: 1074-1080. Heidari, A., S.A. Mohammadi, M. Moghadam, M.R. Shakiba, K. Ghasemi Gholezani, and A. Yousefi. 2011. Analysis of genetic diversity in barley genotypes using SSR and EST-SSR markers. Iranian J. Crop Sci., 13:146-156. Hoseini, S.A. 2012. Evaluation the Hordeum spontaneum chemical control in wheat and determine the connection between herbicide efficacy and P450 cytochrome enzyme monooxygenase. PhD thesis in Weed Science. Ferdosi Mashhad University. Pp. 110. Hou, Y.C., Yan, Z.H., Wei,Y.M. and Y.L. Zheng. 2005. Genetic diversity in barley from west China based on RAPD and ISSR analysis. Barley Gen. Ne., 35:9-22. Jakob, S.S., D. Rodder, J.O. Engler, S. Shaaf, H. Ozkan, F.R. Blattner, and B. Kilian. 2014. Evolutionary history of wild barley (Hordeum vulgare subsp. spontaneum) analyzed using multilocus sequence data and paleodistribution modeling. Genome Biol. Evol., 6:685–702. Jamali, M.R. and Jokar, L. 2010. Effect of rotation in Hordeum spontaneum control in Fars province wheat fields. Crop Protect. Agri. Sci. Ind., 24: 99-107. Keshtkar, E., Kordbacheh, F., Mesgaran, M.B., Mashhadi, H.R. and Alizadeh, H.M. 2009. Effects of the sowing depth and temperature on the seedling emergence and early growth of wild barley (Hordeum spontaneum) and wheat. Weed Biol. & Manag. 9: 10–19. Khelestkina E.K., E.G. Pestsova, E. Salina , M.S. Roder, V.S. Abruzova, S.F. Koval and A. Borner. 2002. Genetic mapping and tagging of wheat Genes using RAPD, STS and SSR markers. Cell. Mol. Biol. Lett. Vol.(7). Pp: 795-802. Maleki, M., Naghavi, M.R., Alizadeh, H. and Mardi, M. 2006. Study the genetic diversity of west Iranian Triticum boeoticum wild populations using RAPD and AFLP. MSc thesis in Biotechnology. University of Tehran. Pp. 112. Matus, I.A. and P.M. Hayes. 2002. Genetic diversity in three groups of barley germplasm assessed by simple sequence repeats. Genome. 45: 1095-1106. Mengistu L.W., C.G. Messersmith, & M.J. Christoffers. 2005. Genetic diversity of herbicide-resistant and –susceptible Avena fatua populations in North Dakota and Minnesota. Weed Res. 45: 413–423. Molina-Cano, J.l., C. Gomez-Campo, and J. Conde. 1982. Hordeum spontaneum C. Koch as a weed of barley fields in Morocco. Z. Pflanzenzucht. 88: 161-167. Moseman, J.G, Baenziger,P.F., Kilpatrick, R.A. 1980. Hordeum spontaneum- an overlooked source of disease resistance. Eur. Medit. Cereal Rust Found. 91-93. Nei, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetices. 89:583-590. Peakall R. and Smouse, P.E. 2006. GenAlEx 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol. Ecol. Notes. 6:288–295. Ranjbar, M., M.R. Naghavi and A. Zali. 2007. Evaluation of morphological and molecular diversity in Iranian accessions of Aegilops crassa. M.Sc. thesis in Plant breeding, University of Tehran. Pp. 94. Roder, M.S., V. Korzun, K. Wendehake, J. Plaschke, M.H. Tixier, P. Leroy and M.W. Ganal. 1998. A microsatellite map of wheat. Genetics. 149:2007–2023. Roldan-Ruiz I., Dendauw, J. E., Van Bockstaele, A. Depicker, De M. Loose. 2000. AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Mol. Breed. 6: 125–134. Roy, J.K., Bandopadhyay, R., Rustgi, S., Balyan, H.S. and Gupta, P.K. 2006. Association analysis of agronomically important traits using SSR, SAMPL and AFLP markers in bread wheat. Curr. Sci. 90: 5-10. Saeidi, S., Karimishahri, M.R., Bazoubandi, M., Dehvari, V. and Ghezelseflou, N. 2013. Assessment of genetic diversity of some Hordeum spontaneum C. Koch. genotypes using SSR markers. Proceeding of 5th Iranian weed congress, Karaj, Iran. 1536-1539. Saghai-Maroof, M.A., K.M. Soliman, R.A. Jorgensen, and R.W. Allard. 1984. Ribosomal DNA spacerlength polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics. Proceedings of the National Academy of Sciences. USA. 81: 8014-8018. Shakhatreh, Y., Haddad, N., Alrababah, M., Grando, S., Ceccarelli, S. 2010. Phenotypic diversity in wild barley (Hordeum vulgare L. ssp. spontaneum (C. Koch) Thell.) accessions collected in Jordan. Genet. Resour. Crop Evol.57: 131-146. Varshney, R.K., Marcel, T.C., Ramsay, L., Russell, J., Roder, M.S., Stein, N. R., Waugh. P. Langridge, R.E. Niks, A. Graner. 2007. A high density barley microsatellite consensus map with 775 SSR loci. Theor. Appl. Genet. 114: 1091–1103. Volis, S., S. Mendlinger, Y. Turuspekov, U. Esnazarov, S. Abugalieva, and N. Orlovsky. 2001. Allozyme variation in Turkmenian populations of wild barley (Hordeum spontaneum Koch.). Ann. Bot. 87: 435-446. Yu, Q., A. Collavo, M.Q. Zheng, M. Owen, M. Sattin, & S.B. Powles. 2007. Diversity of acetyl-coenzyme A carboxylase mutations in resistant Lolium populations: evaluation using clethodim. Plant Physiol. 145, 547–558. Zhang, P., Dreisigacker,S., Melchinger,A.E., Rife, J.C., Mujeebkazi, A., Yan-Ginkel, M., Hoisington, D. and Warburton, M.L. 2005. Quantifying hovel sequence. Variation and selective and their backcross –derived lines using SSR markers. Mol Breed. 15: 1-10. Zong-Yun, F., Xian-Jun, L., Yi-Zhang, Z. and Hong-Qing, L. 2006. Genetic diversity analysis of Tibetan wild barley using SSR marker. Acta Genet. Sin. 33: 917-923. Zohary, D. and Hopf, M. 2003. Domestication of plants in the old world: The origin and spread of cultivated plants in West Asia, Europe, and the Nile Valley. Plant Syst. Evol. 236: 227-228. | ||
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