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Ghadiminejad, Zahra, Lotfi, Mohsen, Abdoshah, Mohammad, Mahmoudzadeh Akhijahani, Mohsen, Kermanshahani, Salimeh, Abtin, Alireza, Shamohammadi, Masoud, Yousefi, Alireza. (1405). An Iranian Velogenic Newcastle Disease Virus Genotype VII.1.1: Isolation, Characterization, and Establishment as a Homologous Challenge Strain for Vaccine Efficacy Evaluation. سامانه مدیریت نشریات علمی, (), -. doi: 10.66224/ari.2026.374330.4360
Zahra Ghadiminejad; Mohsen Lotfi; Mohammad Abdoshah; Mohsen Mahmoudzadeh Akhijahani; Salimeh Kermanshahani; Alireza Abtin; Masoud Shamohammadi; Alireza Yousefi. "An Iranian Velogenic Newcastle Disease Virus Genotype VII.1.1: Isolation, Characterization, and Establishment as a Homologous Challenge Strain for Vaccine Efficacy Evaluation". سامانه مدیریت نشریات علمی, , , 1405, -. doi: 10.66224/ari.2026.374330.4360
Ghadiminejad, Zahra, Lotfi, Mohsen, Abdoshah, Mohammad, Mahmoudzadeh Akhijahani, Mohsen, Kermanshahani, Salimeh, Abtin, Alireza, Shamohammadi, Masoud, Yousefi, Alireza. (1405). 'An Iranian Velogenic Newcastle Disease Virus Genotype VII.1.1: Isolation, Characterization, and Establishment as a Homologous Challenge Strain for Vaccine Efficacy Evaluation', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.66224/ari.2026.374330.4360
Ghadiminejad, Zahra, Lotfi, Mohsen, Abdoshah, Mohammad, Mahmoudzadeh Akhijahani, Mohsen, Kermanshahani, Salimeh, Abtin, Alireza, Shamohammadi, Masoud, Yousefi, Alireza. An Iranian Velogenic Newcastle Disease Virus Genotype VII.1.1: Isolation, Characterization, and Establishment as a Homologous Challenge Strain for Vaccine Efficacy Evaluation. سامانه مدیریت نشریات علمی, 1405; (): -. doi: 10.66224/ari.2026.374330.4360

An Iranian Velogenic Newcastle Disease Virus Genotype VII.1.1: Isolation, Characterization, and Establishment as a Homologous Challenge Strain for Vaccine Efficacy Evaluation

Archives of Razi Institute
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 23 آبان 1405    اصل مقاله (625.71 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.66224/ari.2026.374330.4360
نویسندگان
Zahra Ghadiminejad1؛ Mohsen Lotfi* 1؛ Mohammad Abdoshah1؛ Mohsen Mahmoudzadeh Akhijahani1؛ Salimeh Kermanshahani1؛ Alireza Abtin1؛ Masoud Shamohammadi2؛ Alireza Yousefi1
1Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
21.Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran 2.Department of Immunology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran.
چکیده
Background: Newcastle disease (ND) remains a threat to the poultry industry, with genotype VII strains causing outbreaks in vaccinated flocks. Genetic and antigenic differences between vaccine strains (genotypes I/II) and velogenic strains (genotype VII) necessitate homologous challenge strains to assess vaccine efficacy. Objective: This study aimed to isolate, purify, and characterize a velogenic Newcastle disease virus (NDV) isolate from Iran to establish a homologous challenge strain for vaccine efficacy studies. Methods: Two velogenic NDV isolates from broiler farms in Iran were plaque-purified on Vero cells to obtain clones. Seven clones were characterized using RT-PCR targeting the fusion (F) gene, sequencing, and phylogenetic analysis. Pathogenicity was determined by Mean Death Time (MDT) in embryonated SPF eggs and Intracerebral Pathogenicity Index (ICPI) in day-old SPF chicks. Results: All seven clones belonged to genotype VII, specifically sub-genotype VII.1.1, demonstrating >99.9% nucleotide identity with the parental isolates. The ¹¹²RRQKRF¹¹⁷ cleavage site motif confirmed the velogenic nature of all clones. Pathogenicity revealed MDT values ranging from 45.5 to 55.6 hours (mean ± SD: 50.8 ± 4.2 hours) and ICPI values from 1.86 to 1.97 (mean ± SD: 1.91 ± 0.05). Isolate Ck/IR/ABD/2018 exhibited an ICPI of 1.97 and MDT of 56 hours, approximating the hypervirulent standard strain Herts33/56 (ICPI=2.0). Conclusion: This study introduces Ck/IR/ABD/2018 as the first officially recommended, well-characterized, highly virulent homologous challenge strain for evaluating NDV vaccine efficacy in Iran. Its genetic and phenotypic similarity to circulating field viruses provides a more accurate assessment of vaccine performance under local conditions.
کلیدواژه‌ها
Newcastle disease virus؛ Avian paramyxovirus type 1؛ Genotype VII؛ Challenge strain؛ Vaccine efficacy؛ Pathotyping؛ Phylogenetic analysis
آمار
تعداد مشاهده مقاله: 3


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