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Molecular characterization of Leptospira vaccine serovars using the MLST technique | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 23 آبان 1405 اصل مقاله (1.1 M) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.66224/ari.2026.373364.4197 | ||
| نویسندگان | ||
| Mohadese Tadayon1؛ Pejvak Khaki* 2؛ Mojtaba noofeli3 | ||
| 1Department of Microbiology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iranarak | ||
| 2Department of Microbiology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. | ||
| 3Department of Research and Development, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| چکیده | ||
| Introduction: Leptospirosis is a widespread zoonotic disease causing substantial morbidity and mortality in humans and animals globally. Understanding genetic diversity, verifying strain authenticity, and ensuring quality control of biological products rely heavily on the molecular characterization of vaccine seed serovars. Materials and Methods: In this study, five Leptospira vaccine serovars maintained at the Razi Vaccine and Serum Research Institute were characterized using MLST scheme 3 based on six housekeeping loci. Following PCR amplification and Sanger sequencing, allelic profiles and sequence types (STs) were determined, concatenated, and compared with 15 reference strains from GenBank. Results: Among the vaccination strains, three different species were found: L. interrogans, L. kirschneri, and L. borgpetersenii. L. interrogans serovar Pomona and L. kirschneri serovar Grippotyphosa were found to have two new genotypes. Phylogenetic study demonstrated that L. interrogans serovars Icterohaemorrhagiae (ST1), Pomona (new ST), and Canicola (ST31) grouped closely, showing high genetic identity. L. borgpetersenii serovar Hardjo type bovis (ST145), on the other hand, showed clear phylogenetic separation from the other species. Conclusion: This study demonstrates the first application of MLST to characterize Leptospira vaccine serovars in Iran. Comparative analysis confirmed that these vaccine serovars represent the three major pathogenic species prevalent in humans and livestock globally and domestically. By characterizing the genetic identity of these vaccine strains, this work indirectly aids in future vaccine optimization and quality assurance. Furthermore, the identification of novel sequence types emphasizes the necessity of continuous molecular surveillance and routine PubMLST curation. | ||
| کلیدواژه‌ها | ||
| Iran؛ leptospira؛ MLST؛ phylogenetic analysis؛ vaccine strains | ||
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آمار تعداد مشاهده مقاله: 2 |
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