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Molecular characterization of the resistome in bacterial isolates from canine cutaneous infections. | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 23 آبان 1405 اصل مقاله (840.7 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.66224/ari.2026.373303.4185 | ||
| نویسندگان | ||
| Christian Camilo Patiño Valencia1؛ Maria Alejandra A Bautista Paez1؛ Valentina Rueda Garcia1؛ Iang Schroniltgen Rondón Barragán* 2 | ||
| 1Department of Animal Health, Research Group in Immunobiology and Pathogenesis, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Ibagué-Tolima, Colombia | ||
| 2Department of Animal Health, Research Group in Immunobiology and Pathogenesis, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Ibagué-Tolima, Colombia Department of Animal Health, Poultry Research Group, Faculty | ||
| چکیده | ||
| Introduction: Antimicrobial resistance is one of the most significant challenges in contemporary medicine, affecting human and animal health, agriculture, and the environment. This issue has become increasingly critical due to the rising prevalence of antimicrobial-resistant cases, which has led to adverse consequences, including prolonged hospital stays, increased treatment costs, and higher morbidity and mortality rates within the population. Material and Methods: A total of 30 bacterial isolates were analyzed. The isolates were biochemically characterized, and their phenotypic antimicrobial resistance was determined, showing a predominance of resistance to β-lactam antibiotics. After total DNA extraction, the presence of resistance genes to different antimicrobial classes was evaluated using specific primers and end-point PCR. Results: The analyzed isolates showed the presence of resistance genes, with the highest frequencies observed for β-lactams (blaCMY-2 and blaTEM), trimethoprim (dfrA1), sulfonamides (sul1), quinolones (qnrS), and tetracyclines (tetB). Significant associations (p < 0.05) were observed between most resistance genes and their corresponding phenotypic profiles, indicating a high genotype–phenotype concordance. Conclusions: The rational use of antimicrobials, combined with antimicrobial susceptibility testing and molecular methods, may help limit the spread of bacterial resistance. Finally, the association between genotypic and phenotypic resistance profiles provided a better understanding of the resistance mechanisms present in the studied isolates. | ||
| کلیدواژه‌ها | ||
| Antimicrobial resistance؛ canine skin infections؛ resistome؛ resistance bacteria؛ molecular biology | ||
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