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The Effect of Propolis Extracts on Healthy Vascular Aging Mechanism by The Telomere Regulation in HUVEC Cells | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 19 آبان 1405 اصل مقاله (741.95 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.66224/ari.2026.373791.4279 | ||
| نویسندگان | ||
| Zeliha Selamoglu* 1؛ Seyda Kaya2؛ Sevgi Durna Daştan3؛ Taner Dastan4؛ Ainash Oshibayeva5؛ Yerbol Baltabay6 | ||
| 1Department of Medical Biology, Medicine Faculty, Nigde Omer Halisdemir University, Nigde, Türkiye | ||
| 2Department of Medical Services and Techniques, Medical Laboratory Techniques Program, Vocational School of Health Services, Gaziantep Islam Science and Technology University, Gaziantep / Türkiye | ||
| 3Department of Biology, Faculty of Science, Sivas Cumhuriyet University, Sivas, Türkiye | ||
| 4Department of Biochemistry, Faculty of Science, Sivas Cumhuriyet University, Sivas, Türkiye | ||
| 5Department of Public Health and Scientific Research, Faculty of Medicine, Khoja Akhmet Yassawi International KazakhTurkish University, Turkestan, Kazakhstan | ||
| 6Department of Biology, Faculty of Sciences, Khoja Akhmet Yassawi International Kazakh- Turkish University, Turkestan, Kazakhstan | ||
| چکیده | ||
| Introduction: Propolis is a very important natural bioactive product with potent antioxidant properties; however, the effects of Kazakhstan propolis on telomere-associated molecular pathways remain largely unexplored. The objective was to comparatively evaluate the antioxidant activity, total phenolic and flavonoid contents, cytotoxicity, and telomere-related gene expression profiles of different solvent extracts obtained from propolis collected from four regions of southern Kazakhstan. Methods: Ethanolic, methanolic, water, and ethyl acetate extracts of four propolis samples (Pro1–Pro4) were prepared. Total phenolic content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, total flavonoid content, and HUVEC cell viability were determined. Relative expression levels of TERT, TERF1, TERF2, and PINX1 were quantified by qRT-PCR using the fold change ΔΔCt method with GAPDH. Results: Methanolic and ethanolic extracts generally showed the highest phenolic and flavonoid contents and antioxidant activity. All extracts showed low cytotoxicity toward HUVEC cells, indicating good cellular compatibility. Gene expression analysis demonstrated that methanol extracts increased TERF1 and TERT expression, particularly in the Pro2 and Pro3 samples, whereas ethanol extracts produced intermediate responses, and aqueous extracts showed comparatively weaker effects. TERF2 showed a significant overall group effect, without significant pairwise differences, while PINX1 showed variable but non-significant alterations among treatment groups. One-way ANOVA revealed significant differences for TERF1, TERT, and TERF2. Conclusions: These data suggest that phenolic-rich propolis extracts may support endothelial health and are associated with altered expression of telomere-related genes, and highlight Kazakhstan propolis as a promising source of bioactive compounds for further vascular-aging research. | ||
| کلیدواژه‌ها | ||
| Anti-aging؛ Antioxidant activity؛ Endothelial cells؛ HUVEC؛ Kazakhstan propolis؛ qRT-PCR؛ Telomere | ||
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