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Biocompatible fish-targeted chitosan-TPP/CaP hybrid nanocarrier (Cs/Tpp-Cap) for oral DNA vaccine delivery and immune gene expression in goldfish | ||
| Iranian Journal of Fisheries Sciences | ||
| دوره 25، شماره 6، بهمن 2026، صفحه 1001-1017 اصل مقاله (582.7 K) | ||
| شناسه دیجیتال (DOI): 10.22092/ijfs.2026.136498 | ||
| نویسندگان | ||
| M.B. Balaji1؛ E. Thirumalaikumar* 2؛ S. Vimal* 3؛ S. Abdul Majeed4 | ||
| 1Department of Biochemistry, Saveetha Medical College & Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Thandalam, Chennai - 602105, Tamil Nadu, India | ||
| 2Centre for Aquaculture, Sathyabama Institute of Science and technology, Chennai -600119, Tamilnadu, India | ||
| 3Department of Biochemistry, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Thandalam, Chennai - 602105, Tamil Nadu, India | ||
| 4Aquatic Animal Health Laboratory, PG and Research Department of Zoology, C Abdul Hakeem College, Melvisharam, Tamil Nadu, 632509, India | ||
| چکیده | ||
| This study evaluates the potential of chitosan tripolyphosphate/calcium phosphate (Cs/Tpp-Cap) nanoparticles as carriers for oral DNA vaccine delivery in fish. In aqueous solution, positively charged Cs/Tpp-Cap nanoparticles interact electrostatically with negatively charged DNA molecules, resulting in the spontaneous self-assembly of stable nanocomplexes. This self-assembly strategy provides an innovative means of modifying anionic Cs/Tpp-Cap hybrid nanoparticles for effective oral DNA vaccine applications. The DNA vaccine was constructed by cloning the Vibrio anguillarum outer membrane porin (OMP) gene (993 bp) into the eukaryotic expression vector pcDNA3.1, generating the recombinant plasmid pVAOMP DNA. The morphology and structural characteristics of Cs/Tpp-Cap nanoparticles were characterized using scanning electron microscopy (SEM), high-resolution transmission electron microscopy (TEM), and Fourier-transform infrared spectroscopy (FTIR). For encapsulation, equal volumes of heated Cs/Tpp-Cap and pVAOMP DNA solutions were combined, achieving a DNA binding efficiency of 85%. Gene distribution and expression analyses confirmed the successful incorporation of the VAOMP gene and revealed the upregulation of immune-related genes in vaccinated fish. These findings demonstrate that Cs/Tpp-Cap nanoparticles can function as an efficient, biocompatible, and non-viral delivery system for oral DNA vaccination in aquaculture species, providing a promising platform for disease prevention in fish farming. | ||
| کلیدواژهها | ||
| Chitosan tripolyphosphate/calcium phosphate nanoparticles؛ In vivo tissue distribution؛ pVAOMP DNA؛ In vivo gene expression | ||
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