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Oomycetes associated with cucurbit rhizospheres in Iran: A new record of Pythium tardicrescens | ||
| Mycologia Iranica | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 19 مهر 1405 اصل مقاله (1.9 M) | ||
| نوع مقاله: Short Article | ||
| شناسه دیجیتال (DOI): 10.22092/mi.2026.373643.1352 | ||
| نویسندگان | ||
| Fatemeh Salmaninezhad؛ Pouria Sekandarpour؛ Reza Mostowfizadeh-Ghalamfarsa* | ||
| Department of Plant Protection, School of Agriculture, Shiraz University, Shiraz, Iran | ||
| چکیده | ||
| Cucurbits are widely cultivated in Iran, particularly under intensive greenhouse production, where soilborne oomycetes may contribute to root and crown diseases. During a survey of cucurbit production systems in Fars Province in 2025, symptomatic root and crown tissues, rhizosphere soils, plant debris, and irrigation water were collected from cucumber, cantaloupe, muskmelon, squash, and watermelon. Oomycetes were isolated using selective media and baiting methods, and representative isolates were characterized based on cultural and morphological features, cardinal temperatures, pathogenicity assays, and molecular phylogenetic analyses. Among recovered oomycete isolates, seven were identified as Phytophthora inundata, Phytopythium litorale, and Pythium tardicrescens. Molecular analyses confirmed their placement in Clade 6a of Phytophthora, Clade 1 of Phytopythium, and Clade B of Pythium sensu stricto, respectively. Five isolates of Py. tardicrescens were recovered from diseased squash rhizosphere soil, representing the first record of this species from Iran. The species was characterized by slow radial growth, delayed formation of reproductive structures, filamentous to lobate sporangia, globose terminal oogonia, monoclinous or paragynous antheridia, and aplerotic oospores. A single isolate of Ph. inundata was recovered from cucumber rhizosphere soil, representing the first association of this species with cucumber worldwide. Phytopythium litorale was also recovered from cucumber rhizosphere soil. None of the isolates caused symptoms in cucurbit seedlings tested under the experimental conditions used. These findings document Py. tardicrescens in Iran for the first time and a new cucumber association for Ph. inundata and highlight the need for broader ecological and pathogenicity studies to clarify their roles in cucurbit production systems. | ||
| کلیدواژه‌ها | ||
| Cucurbitaceae؛ Oomycota؛ Pathogenicity؛ Phylogeny؛ Taxonomy | ||
| مراجع | ||
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