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Mechanistic Evaluation of the Antidiabetic Potential of Kazakhstan Honey through in vitro and in silico Approaches | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 05 مهر 1405 اصل مقاله (1.31 M) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.66224/ari.2026.373647.4244 | ||
| نویسندگان | ||
| Zeliha Selamoglu* 1؛ Seyda Kaya2؛ Eda Sonmez Gurer3؛ Sevgi Durna Daştan4؛ Ainash Oshibayeva5؛ Furkat Ubaev6؛ Taner Dastan7؛ Nurdana Salybekova8 | ||
| 1Nigde Omer Halisdemir University | ||
| 2Department of Medical Services and Techniques, Medical Laboratory Techniques Program, Vocational School of Health Services, Gaziantep Islam Science and Technology University, Gaziantep / Türkiye | ||
| 3Department of Pharmacy Vocational Sciences, Faculty of Pharmacy, Sivas Cumhuriyet University, Sivas, Türkiye | ||
| 4Department of Biology, Faculty of Science, Sivas Cumhuriyet University, Sivas, Türkiye | ||
| 5Department of Public Health and Scientific Research, Faculty of Medicine, Khoja Akhmet Yassawi International KazakhTurkish University, Turkestan, Kazakhstan | ||
| 6Center for Sustainable Development and Scientific Research, Khoja Akhmet Yassawi International Kazakh-Turkish University, Turkestan, Kazakhstan | ||
| 7Department of Biochemistry, Faculty of Science, Sivas Cumhuriyet University, Sivas, Turkiye | ||
| 8Department of Biology, Faculty of Sciences, Khoja Akhmet Yassawi International Kazakh- Turkish University, Turkestan, Kazakhstan | ||
| چکیده | ||
| Introduction: This study evaluated the antioxidant, antidiabetic, and antiglycation properties of honey samples from southern Kazakhstan and investigated their underlying molecular mechanisms using integrated in vitro and in silico approaches. Methods: Honey samples were evaluated for total phenolic and flavonoid contents, DPPH radical scavenging, α-glucosidase inhibition, and bovine serum albumin glycation inhibition. Representative phenolic compounds were further analyzed by network pharmacology, protein–protein interaction analysis, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment, and molecular docking. Results: DPPH radical scavenging IC₅₀ values ranged from 157.9 to 337.8 μg/mL. Total phenolic and flavonoid contents ranged from 98.3–116.7 mg GAE/g and 5.3–6.9 mg QE/g extract, respectively. BSA glycation and α-glucosidase inhibition IC₅₀ values ranged from 318–443 and 36.7–65.3 μg/mL, respectively. HC showed the antioxidant activity (IC₅₀ = 157.9 μg/mL) and α-glucosidase inhibitory (IC₅₀ = 37.9 μg/mL) activities, whereas H13 exhibited the highest antiglycation activity (IC₅₀ = 318 μg/mL). Computational analyses identified STAT3, EGFR, ALB, and SRC as hub proteins, highlighted the PI3K–Akt pathway, and demonstrated favorable docking interactions and pharmacokinetic properties of the investigated phenolic compounds. Conclusion: This study provides the first integrated experimental and computational evidence supporting the multitarget antidiabetic potential of Kazakhstan honey, highlighting its promise as a functional food and a source of natural lead compounds for nutraceutical and pharmaceutical applications. | ||
| کلیدواژهها | ||
| α-glucosidase inhibition؛ BSA glycation inhibition؛ In silico؛ Kazakhstan Honey؛ T2DM | ||
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