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بررسی اثر ضد میکروبی فیلم نانوکیتوزان- کربوکسی متیل سلولز حاوی نانو ذرات نقره و اسانس خوشاریزه | ||
| تحقیقات مهندسی صنایع غذایی | ||
| دوره 25، شماره 2، شهریور 1405، صفحه 49-60 اصل مقاله (719.75 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/fooder.2026.372953.1459 | ||
| نویسندگان | ||
| اله یار نبی پور1؛ علی خنجری* 2؛ ژاله خوشخو1؛ افشین آخوندزاده بستی3؛ مرجانه صداقتی1 | ||
| 1گروه علوم و صنایع غذایی، دانشکده علوم زیستی، واحد تهران شمال،، دانشگاه آزاد اسلامی ،تهران، ایران | ||
| 2دانشگاه تهران | ||
| 3گروه بهداشت و کنترل مواد غذایی، دانشکده دامپزشکی، دانشگاه تهران ،تهران، ایران | ||
| چکیده | ||
| این مطالعه با هدف بررسی اثر ضد میکروبی فیلم نانوکیتوزان–کربوکسی متیل سلولز حاوی نانو ذرات نقره و اسانس خوشاریزه (Echinophora sibthorpiana) علیه برخی باکتریهای مهم غذازاد بهاجرا در آمد. اسانس خوشاریزه از اندام های هوایی گیاه با روش تقطیر با آب و دستگاه کلونجر استخراج شد. نانو ذرات نقره به روش زیستی تهیه شد و اسانس ها با غلظت مشخص اضافه گردید. فیلمهای نانوکیتوزان–کربوکسی متیل سلولز در چهار تیمار شامل: پایه، حاوی نانو نقره، حاوی نانو نقره و ۳/۰ درصد اسانس، و حاوی نانو نقره و ۶/۰ درصد اسانس ساخته شدند. اثر ضد میکروبی این فیلمها علیه سالمونلا تیفی موریوم (Salmonella typhimurium )، اشریشیا کلی (Escherichia coli)، لیستریا مونوسیتوژنز (Listeria monocytogenes) و استافیلوکوکوس اورئوس (Staphylococcus aureus ) ارزیابی شد. دادهها با نرم افزار SPSS 26 و آزمون های ANOVA و توکی تحلیل شدند. نتایج نشان داد با افزودن نانو نقره و پس از آن اسانس خوشاریزه به فیلم پایه، قطر هاله عدم رشد افزایش مییابد. بیشترین اثر ضد میکروبی در فیلم حاوی ۶/۰ درصد اسانس خوشاریزه مشاهده شد و باکتریهای گرم مثبت نسبت به گرم منفیها حساسیت بیشتری نشان دادند. نتایج تحقیق نشان داد که نانو ذرات نقره و اسانس خوشاریزه موجب افزایش فعالیت ضد میکروبی فیلمهای نانو کیتوزان-کربوکسی متیل سلولز در شرایط آزمایشگاهی میشوند. برای معرفی این فیلمها به عنوان گزینۀ کاربردی در بستهبندی فعال مواد غذایی، لازم است آزمون های تکمیلی روی مادۀ غذایی صورت پذیرد. | ||
| کلیدواژهها | ||
| اثر ضد میکروبی؛ خوشاریزه؛ کربوکسی متیل سلولز؛ نانو ذرات نقره؛ نانو کیتوزان | ||
| عنوان مقاله [English] | ||
| Antimicrobial Activity of Nanochitosan – Carboxymethyl Cellulose Films Incorporating Silver Nanoparticles and Echinophora sibthorpiana Essential Oil | ||
| نویسندگان [English] | ||
| Allahyar Nabipour1؛ Zhaleh Khoshkhoo1؛ Afshin Akhoondzadeh Basti3؛ Marjaneh Sedaghati1؛ | ||
| 1Department of Food Science and Technology, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran | ||
| 3Department of Food Hygiene and Quality Control,Faculty of Veterinary medicine,Tehran University,Tehran,Iran | ||
| چکیده [English] | ||
| This study aimed to investigate the antimicrobial effects of nano-chitosan/carboxymethyl cellulose (CMC) films incorporated with silver nanoparticles (SNPs) and Echinophora sibthorpiana essential oil against several foodborne pathogens. The essential oil was extracted from the aerial parts of the plant by hydrodistillation using a Clevenger-type apparatus. Subsequently, silver nanoparticles were synthesized via a green method, followed by the incorporation of the essential oil at specific concentrations. Nano-chitosan/CMC films were prepared in four treatments: control (base film), film containing SNPs, film containing SNPs + 0.3% essential oil, and film containing SNPs + 0.6% essential oil. The antimicrobial activity of these films was evaluated against Salmonella typhimurium, Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus. Data were analyzed using SPSS software (version 26) through one-way ANOVA and Tukey’s test. The results demonstrated that the incorporation of silver nanoparticles and, subsequently, the essential oil into the base film significantly increased the inhibition zone diameters. The highest antimicrobial activity was observed in the film containing 0.6% essential oil, and Gram-positive bacteria were more sensitive than Gram-negative strains. The results demonstrated that the incorporation of silver nanoparticles and Echinophora sibthorpiana essential oil enhances the antimicrobial activity of chitosan-carboxymethyl cellulose nanofilm. The results indicated that the incorporation of silver nanoparticles and Echinophora sibthorpiana essential oil enhanced the antimicrobial activity of chitosan-carboxymethyl cellulose nanofilms under in vitro conditions. Furthermore, to introduce these films as practical options for active food packaging, additional in situ testing within real food matrices is required. | ||
| کلیدواژهها [English] | ||
| Antimicrobial activity, Carboxymethyl cellulose, Echinophora sibthorpiana, Nanochitosan, Silver nanoparticles | ||
| مراجع | ||
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