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Mitochondrial Cyt b and CR differentiation and in silico RFLP markers for Bangladeshi and Vietnam-originated Climbing Perch (Anabas testudineus) | ||
| Iranian Journal of Fisheries Sciences | ||
| دوره 25، شماره 5، آذر 2026، صفحه 895-915 اصل مقاله (1013.54 K) | ||
| شناسه دیجیتال (DOI): 10.22092/ijfs.2026.136196 | ||
| نویسندگان | ||
| Md.A. Shahariar1؛ S. Aditya1؛ Md.M. Hassan1؛ T. Saulin1؛ A. Arif2؛ S.R. Deo3؛ T. Sharmin1؛ S. Nahar4؛ M.S. Alam* 1 | ||
| 1Department of Genetics and Fish Breeding, Gazipur Agricultural University, Gazipur-1706, Bangladesh | ||
| 2Grameen Jano Unnayan Sangstha (GJUS), Charnoabad, Bhola, Bangladesh | ||
| 3Upazila Fisheries Office, Birol, Dinajpur, Bangladesh | ||
| 4Researcher V, Department of Laboratory Medicine, UAB Heersink School of Medicine, Birmingham, Alabama, United States of America | ||
| چکیده | ||
| This study employed mitochondrial Cytochrome b (Cyt b) and Control Region (CR) markers to investigate the genetic diversity and population structure of Bangladeshi and Vietnam-origin climbing perch (Anabas testudineus) populations across Bangladesh. A total of 320 individuals were collected from 16 sampling sites representing all eight administrative divisions of Bangladesh. Following quality trimming, 818 bp of Cyt b and 705 bp of CR sequences were analyzed, revealing 9 and 28 haplotypes, respectively. Analysis of molecular variance (AMOVA) indicated that the majority of genetic variation was attributable to differences among populations (Cyt b: 95.6%; CR: 89.5%). Additionally, substantial genetic differentiation was observed between Bangladeshi and Vietnam-origin groups (Cyt b: 67.5%; CR: 73.0%). Pairwise Fst values (Cyt b: 0.815; CR: 0.804) confirmed strong differentiation between Bangladeshi and Vietnam-originated groups. Bayesian phylogeny and haplotype network analyses separated Bangladeshi and Vietnam-origin stocks into distinct clades. Shared haplotypes in Dhaka and Chattogram indicated admixture with Vietnam-originated stocks. Several unique haplotypes were identified in both Bangladeshi and Vietnam-originated populations, which may serve as markers for stock identification and broodstock management. In silico RFLP analysis identified seven enzymes (AciI, HaeIII, HhaI, MboI, MspI, RsaI, and StuI) for Cyt b sequences as cost-effective, practical tools for stock identification, while no diagnostic enzymes were found for CR. These findings provide a genetic baseline for conservation and selective breeding, and highlight the urgent need to protect Bangladeshi A. testudineus from admixture with exotic strains. | ||
| کلیدواژهها | ||
| Bangladesh, Climbing perch, Control Region, Cyt b؛ mtDNA, Population structure, RFLP markers | ||
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