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Biosynthesis of Silver Nanoparticles Using Oleander (Nerium oleander) Extract and Its Effect on the Expression of the TEM Gene in Escherichia coli Causing urinary tract infections | ||
| Journal of Medicinal Plants and By-products | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 24 شهریور 1405 اصل مقاله (3.27 M) | ||
| نوع مقاله: Research Article | ||
| شناسه دیجیتال (DOI): 10.22034/jmpb.2027.373416.2227 | ||
| نویسندگان | ||
| Nahid Heydari1؛ Zeinab Fotoohiyan2؛ Mohammad Shariati3؛ Ali Salehi Sardoei4؛ Reza Ghasemi5؛ Hamidreza Balouchi6؛ Bahman Fazeli-Nasab* 7 | ||
| 1Department of Microbiology Plant Pathology, Ji.c., Islamic Azad University, Jiroft, Iran | ||
| 2Department of Plant Pathology, Ji.c., Islamic Azad University, Jiroft, Iran | ||
| 3Jiroft Social Security Clinic, Jiroft, Iran | ||
| 4Crop and Horticultural Science Research Department, South Kerman Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Jiroft, Iran | ||
| 5Department of Environmental Health Engineering, Tehran University of Medical Science, Tehran, Iran | ||
| 6Agronomy and Plant Breeding Department, Faculty of Agriculture, Yasouj University, Yasouj, Iran | ||
| 7Department of Agronomy and Plant Breeding, Agriculture Institute, Research Institute of Zabol, Zabol, Iran | ||
| چکیده | ||
| E. coli’s TEM gene-mediated resistance to beta-lactams challenges UTI treatment. This study explores oleander-synthesized silver nanoparticles as a novel antimicrobial strategy against resistant strains. To assess TEM gene prevalence in E. coli isolates and evaluate oleander-based AgNPs and methyltransferase interactions for combating antibiotic resistance. A total of 155 UTI-associated E. coli isolates were identified via biochemical tests and ESBL screening (CLSI guidelines). TEM gene detection used PCR with specific primers. Oleander-synthesized AgNPs were characterized (UV-Vis) and tested for antimicrobial activity (disk diffusion, MIC/MBC). Protein-protein docking analyzed blaTEM (KX462012.1) and oleander methyltransferase (MH504111.1) interactions. Experimental data analyzed via randomized ANOVA, Duncan's test (p<0.05), PFAFFL. Among 155 E. coli isolates from UTIs, 65% (100 strains) produced ESBLs, with highest resistance to ampicillin (74%), carbenicillin (61%), and chloramphenicol (53%). PCR confirmed the blaTEM gene in 60% of tested isolates. Oleander-synthesized silver nanoparticles (AgNPs) exhibited potent antimicrobial activity, showing dose-dependent inhibition zones (10–40 µg/mL), with MIC and MBC values of 16 µg/mL and 32 µg/mL, respectively. Real-time PCR revealed a 48.5% reduction in blaTEM expression (*p* < 0.05) after AgNP treatment. Molecular docking demonstrated stable binding between blaTEM and oleander methyltransferase, driven by hydrophobic interactions (Leu, Ile, Val, Phe, Trp), with Trp playing a pivotal role. Ramachandran plots validated high-quality 3D structures (94–96% favored residues) for both proteins. The study isolated 155 E. coli strains (65% ESBL-producing) from UTIs, showing high antibiotic resistance. Oleander-synthesized silver nanoparticles (MIC: 16 µg/mL) inhibited TEM gene-carrying E. coli and reduced TEM expression by 48.5%. Oleander methyltransferase stably binds blaTEM via hydrophobic interactions, validated by high-quality 3D modeling and docking studies. | ||
| کلیدواژه‌ها | ||
| Escherichia coli؛ Nerium oleander؛ ESBL؛ TEM gene؛ In Silico | ||
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آمار تعداد مشاهده مقاله: 107 تعداد دریافت فایل اصل مقاله: 110 |
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