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Serological Detection of Toxoplasma gondii in Packed Red Blood Cells Using Soluble Recombinant Dense Granule Antigen (rGRA7), with Confirmatory Nested-PCR Performed on ELISA-Positive Samples | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 14 شهریور 1405 اصل مقاله (954.34 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.66224/ari.2026.372677.4104 | ||
| نویسندگان | ||
| Hadi Mirahmadi1؛ Marziye Shahraki Zare2؛ Alireza Salimi-Khorashad2؛ Ebrahim Alijani3؛ Abolhassan Safdari4؛ Ahmad Mehravaran5؛ Reza Shafiei6؛ Seyed Ghader Azizi* 3 | ||
| 1Clinical Immunology Research Center, Zahedan University of Medical Sciences, Zahedan, Iran Department of Parasitology and Mycology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran | ||
| 2Department of Parasitology and Mycology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran | ||
| 3Clinical Immunology Research Center, Zahedan University of Medical Sciences, Zahedan, Iran | ||
| 4Department of Infectious Disease, School of Medicine Infectious Diseases and Tropical Medicine Research Center, Zahedan, Iran | ||
| 5Department of Parasitology and Mycology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran Infectious Diseases and Tropical Medicine Research Center, Research Institute of Cellular and Molecular Science in | ||
| 6Vector-borne Diseases Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran | ||
| چکیده | ||
| Introduction: Toxoplasmosis is a prevalent and severe but significantly overlooked parasitic disease, which can be transmitted via blood products. The current study aimed to assess the possible contamination of packed red blood cells (PRBCs) with Toxoplasma gondii (T. gondii) and the applicability of combined serological (ELISA) and molecular (Nested-PCR) methods for its detection. Material and methods: After plasmid transformation and GRA7 protein production, the purified protein was used to develop an ELISA kit. IgG and IgM antibodies against T. gondii were detected via a GRA7 antigen-based ELISA. In serologically positive samples, Nested-PCR was used to verify the parasite by amplifying a 164 bp fragment within a larger 529 bp repetitive element (RE) sequence of T. gondii. Results: Out of all tested samples, 35 (1.75 %) were positive for IgM, 402 (20.1 %) were positive for IgG, and 14 (0.7 %) samples showed simultaneous positivity for both IgG and IgM. Notably, within the 423 antibody-positive PRBCs, 82 units (4.1%) underwent leukocyte reduction. Four PRBC units that were non leukocyte reduced were positive for T. gondii DNA. Conclusion: Due to the limited sensitivity of serological assays in detecting T. gondii, the use of complementary molecular diagnostic techniques is essential for accurate identification. Also, practical procedures such as using leukocyte reducing filters for high-risk blood recipients and implementing a strict donor screening process are necessary to mitigate the transmission of Toxoplasma. | ||
| کلیدواژهها | ||
| ELISA؛ Packed RBC؛ PCR؛ rGRA7؛ Toxoplasma gondii | ||
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