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Exploring Bacterial Attachment on Modified Polycaprolactone-Polyaniline-Gelatin Nanofibers for Innovative Detection Strategies | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 20 مرداد 1405 اصل مقاله (1.57 M) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.66224/ari.2026.372329.4068 | ||
| نویسندگان | ||
| Ali KarbalaeiMahdi1؛ Atefeh Derakhshani2؛ Kaykhosro Moridi3؛ Mohsen Abedini Esfahlani2؛ Danial Ashiani4؛ Mohammad Hossein Farhid5؛ Reza Faridi-Majidi* 6 | ||
| 1Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. | ||
| 2Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. | ||
| 3Department of Biosciences, Università degli studi di Milano, Milan, Italy. | ||
| 4The Research Institute of Petroleum Industry, Tehran, Iran | ||
| 5Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. | ||
| 6Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran Universityof Medical Sciences, Tehran, Iran | ||
| چکیده | ||
| Introduction: Detecting pathogenic bacteria quickly and accurately is essential for food safety and clinical diagnostics. Biosensors offer a fast, cost-effective alternative to traditional microbial identification. This study describes a novel nanofiber-based biosensor designed to sensitively detect Escherichia coli (E. coli). Materials and Methods: Electrospun nanofibers composed of polycaprolactone (PCL), gelatin (Gel), and polyaniline (PANi) were fabricated to create a biocompatible scaffold. Biotinylated bacteriophages, serving as highly specific bioreceptors for E. coli, were covalently immobilized onto the surface of the prepared nanofibers. This immobilization strategy employed avidin-biotin interactions mediated by EDC/ NHS crosslinking chemistry, which activated carboxyl groups on the nanofiber surface to enable stable and oriented bioconjugation. The capture efficiency and specificity of the functionalized platform were subsequently evaluated against target E. coli cells using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Results: Raman spectral analysis confirmed the presence of characteristic peaks corresponding to amide and amine (NH) functional groups on the nanofiber surface following phage immobilization, verifying successful chemical modification. Qualitative analysis using SEM and AFM provided direct visual evidence of bacterial attachment onto the bacteriophage-functionalized nanofiber mats, demonstrating the platform's capacity for effective microbial capture. Conclusion: The integration of conductive PANi with a biocompatible PCL/Gel scaffold, combined with the specificity of phage functionalization, establishes an effective interface for bacterial detection. This innovation signifies a pivotal advancement in pathogen detection methodologies and biosensor technologies, offering a promising tool for applications requiring rapid and reliable identification of microbial contaminants. | ||
| کلیدواژهها | ||
| Bacteriophage T4؛ Biosensor؛ E. coli؛ Electrospinning؛ PANi | ||
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آمار تعداد مشاهده مقاله: 65 تعداد دریافت فایل اصل مقاله: 41 |
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