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AvrStb6 diversity and isoform variation in Zymoseptoria tritici, the fungal wheat pathogen from Golestan Province, Iran | ||
| Mycologia Iranica | ||
| دوره 13، شماره 1، شهریور 2026، صفحه 39-50 اصل مقاله (1.14 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22092/mi.2026.372598.1346 | ||
| نویسندگان | ||
| Vala Rezvani1؛ Parissa Taheri2؛ Mohammad Javan-Nikkhah1؛ Naser Mohammadi3؛ Amir Mirzadi Gohari* 1 | ||
| 1Department of Plant Protection, Faculty of Agriculture, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran | ||
| 2Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. | ||
| 3Dryland Agricultural Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Maragheh, Iran | ||
| چکیده | ||
| Septoria tritici blotch (STB), caused by Zymoseptoria tritici, is a major disease of wheat worldwide. The avirulence effector AvrStb6 is central to the gene-for-gene interaction with the wheat resistance protein Stb6. In this study, the AvrStb6 locus was analyzed in three isolates from Golestan Province, Iran. Sequencing identified two haplotypes that encoded two distinct protein isoforms of 82 amino acids. Structural modelling using SWISS-MODEL showed that many nonsynonymous substitutions were located in surface-exposed loops, potentially affecting effector–host interactions. Pathogenicity tests revealed a clear association between isoform sequence and virulence: an I01‑like isoform produced very low pycnidial coverage on Stb6‑containing wheat, whereas divergent isoforms produced high pycnidial coverage. Notably, the persistence of an I01‑like isoform in Iran contrasts with global reports of its absence. Global phylogenetic analysis placed the Golestan haplotypes within Turkish–European lineages. These findings demonstrate rapid effector diversification in Golestan province and emphasize the importance of diversified resistance breeding for durable STB management. | ||
| کلیدواژه‌ها | ||
| Avirulence؛ Effector isoforms؛ Phylogenetic analysis؛ Protein structure modelling؛ Septoria tritici blotch | ||
| مراجع | ||
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