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Identification of Canariomyces notabilis associated with Ficus carica branch bark discoloration | ||
| Rostaniha | ||
| دوره 27، شماره 1 - شماره پیاپی 74، آبان 2026، صفحه 35-47 اصل مقاله (1.12 M) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22092/bot.j.iran.2026.372496.1450 | ||
| نویسندگان | ||
| Hamed Negahban1؛ Moslem Jafari2؛ Reza Mostowfizadeh-Ghalamfarsa* 3 | ||
| 1PhD Candidate, Department of Plant Protection, School of Agriculture, Shiraz University, Shiraz, Iran | ||
| 2Assistant Prof., Fig Research Station, Fars Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Estahban, Iran | ||
| 3Prof., Department of Plant Protection, School of Agriculture, Shiraz University, Shiraz, Iran | ||
| چکیده | ||
| This study, reports the first isolation and characterization of Canariomyces notabilis from Iran, an emerging pathogenic fungus associated with bark discoloration of branches in fig trees (Ficus carica). Morphological, cultural, and molecular analyses were employed to identify isolates obtained from symptomatic bark. Phylogenetic analysis of tub2 gene sequences using Bayesian inference and maximum likelihood methods robustly placed the isolates within the C. notabilis clade, demonstrating a close relationship with the type strain. To establish pathogenicity, Koch’s postulates were fulfilled through controlled inoculation experiments. Inoculation of detached fig shoots with mycelial plugs consistently induced brown necrosis within eight days. Similarly, conidial suspensions applied to detached leaves resulted in necrotic and chlorotic lesions within one week. Inoculation of one-year-old fig saplings using conidial suspensions revealed the pathogen’s capability to cause external lesions and internal vascular discoloration, extending both proximally and distally from inoculation sites over three months. Successful re-isolation of the fungus from symptomatic tissues confirmed its etiological role. This research establishes C. notabilis as a new fungal species for the funga of Iran and identifies it as an emerging pathogen of fig trees, representing the first documented report of this species as a plant pathogen. | ||
| کلیدواژهها | ||
| β‑tubulin gene؛ Chaetomiaceae؛ emerging pathogen؛ molecular phylogenetics؛ Moraceae | ||
| عنوان مقاله [English] | ||
| شناسایی Canariomyces notabilis همراه با تغییر رنگ پوست شاخه Ficus carica | ||
| نویسندگان [English] | ||
| حامد نگهبان1؛ مسلم جعفری2؛ رضا مستوفی زاده قلمفرسا3 | ||
| 1دانشجوی دکتری بخش گیاهپزشکی، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران | ||
| 2استادیار پژوهش ایستگاه تحقیقات انجیر، سازمان تحقیقات کشاورزی، استهبان، ایران | ||
| 3استاد بخش گیاهپزشکی، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران | ||
| چکیده [English] | ||
| این مطالعه، نخستین گزارش از جداسازی و شناسایی قارچ Canariomyces notabilis از ایران، همراه با علایم نوظهور تغییر رنگ پوست شاخههای انجیر خوراکی (Ficus carica) است. جداسازی از پوست شاخههای علایمدار انجام شد و شناسایی جدایههای به دست آمده با بررسیهای ریختشناختی و مولکولی انجام گرفت. واکاویهای فیلوژنتیکی براساس توالیهای ژن بتاتوبولین (tub2) با استفاده از روشهای استنتاجِ بیزی و درستنمایی بیشینه جدایهها را در تبار C. notabilis در کنار جدایه تیپ این گونه قرار داد. به منظور اثبات بیماریزایی، اصول کُخ از طریق آزمونهای بیماریزایی متعددی انجام شد. مایهزنی شاخههای بریده انجیر با بلوکهای میسیلیومی، منجر به ظهور علایم بافتمردگی و تغییر رنگ چوب در مدت هشت روز پس از مایهزنی شد. همچنین، برگهای مایهزنیشده انجیر با سوسپانسیون هاگ، یک هفته پس از مایهزنی، علایم بافتمردگی و هاله سبزردی را در اطراف محلهای مایهزنی نشان دادند. به علاوه، مایهزنی نهالهای یکساله انجیر با استفاده از سوسپانسیون هاگ، توانایی این بیمارگر را در ایجاد علایم خارجی (شامل لکههای قهوهای روی ساقهها) و همچنین، علایم داخلی (شامل تغییر رنگ بافت آوندی به سمت بالا و پایین از محل مایهزنی) را در یک بازه سهماهه پس از مایهزنی نشان داد. جداسازی دوباره جدایههای مایهزنیشده از حاشیه بافتهای علایمدار، نقش سببشناختی این بیمارگر نوظهور را در علایم مشاهده شده، تأیید کرد. پژوهش حاضر، گونه C. notabilis را به عنوان یک گونه جدید برای قارچهای ایران و یک بیمارگر نوظهور در درختان انجیر معرفی میکند. همچنین، این نخستین مورد مستند از فعالیت این گونه به عنوان یک عامل بیمارگر گیاهی محسوب میشود. | ||
| کلیدواژهها [English] | ||
| بیمارگر نوظهور, تیره توت, ژن بتاتوبولین, فیلوژنتیک مولکولی, Chaetomiaceae | ||
| مراجع | ||
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