Jalili, Leyla, Chaghakaboodi, Zeinab, Zebarjadi, Alireza, Zarei, Leila. (1405). Effect of Plant Growth Regulators and Explant Type on Direct Regeneration and Root Induction of Zarrin-Giah (Dracocephalum kotschyi Boiss) as a Medicinal Plant. سامانه مدیریت نشریات علمی, 15(3), 311-320. doi: 10.22034/jmpb.2026.370541.2042
Leyla Jalili; Zeinab Chaghakaboodi; Alireza Zebarjadi; Leila Zarei. "Effect of Plant Growth Regulators and Explant Type on Direct Regeneration and Root Induction of Zarrin-Giah (Dracocephalum kotschyi Boiss) as a Medicinal Plant". سامانه مدیریت نشریات علمی, 15, 3, 1405, 311-320. doi: 10.22034/jmpb.2026.370541.2042
Jalili, Leyla, Chaghakaboodi, Zeinab, Zebarjadi, Alireza, Zarei, Leila. (1405). 'Effect of Plant Growth Regulators and Explant Type on Direct Regeneration and Root Induction of Zarrin-Giah (Dracocephalum kotschyi Boiss) as a Medicinal Plant', سامانه مدیریت نشریات علمی, 15(3), pp. 311-320. doi: 10.22034/jmpb.2026.370541.2042
Jalili, Leyla, Chaghakaboodi, Zeinab, Zebarjadi, Alireza, Zarei, Leila. Effect of Plant Growth Regulators and Explant Type on Direct Regeneration and Root Induction of Zarrin-Giah (Dracocephalum kotschyi Boiss) as a Medicinal Plant. سامانه مدیریت نشریات علمی, 1405; 15(3): 311-320. doi: 10.22034/jmpb.2026.370541.2042
Effect of Plant Growth Regulators and Explant Type on Direct Regeneration and Root Induction of Zarrin-Giah (Dracocephalum kotschyi Boiss) as a Medicinal Plant
1Department of Plant Production and Genetics, Faculty of Sciences and Agricultural Engineering, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran
2Research Institute of Medicinal Plants, Razi University, Kermanshah, Iran
چکیده
Dracocephalum kotschyi Boiss. (Lamiaceae), known in Persian as “Zarrin-giah” or “Denaei Lemon Balm”, is a medicinal plant native to Iran. It is used in traditional remedies and as a flavoring in tea and doogh (a yogurt-based drink). Extracts of this species contain bioactive compounds with reported therapeutic properties. Efficient micropropagation protocols can support its conservation and large-scale production. This study evaluated plant growth regulator combinations and explant types for direct regeneration and rooting of D. kotschyi. A completely randomized experiment with three replications was conducted in 2025 to investigate the effects of cytokinin (BAP), auxin (NAA), and explant type (leaf, hypocotyl, and cotyledon) on regeneration, as well as the effects of Indole-3-butyric acid (IBA) and culture media (MS and ½ MS) on rooting. Measured traits included regeneration percentage, number of shoots per explant, shoot length, number of leaves per shoot, rooting percentage, number of roots, and root length. Results showed that the combination of 1 mg/L BAP and 1.5 mg/L NAA applied to hypocotyl explants produced the highest regeneration percentage and improved shoot quality. In the rooting stage, the interaction between medium type and IBA concentration significantly influenced root formation. The best rooting response, including 100% rooting, occurred in ½ MS medium supplemented with 1–2 mg/L IBA. These results provide a practical protocol for efficient in vitro regeneration and rooting of D. kotschyi and support its micropropagation for medicinal use and conservation.
Bednark P.T., Orlowska R. Plant tissue culture environment as a switch-key of (epi)genetic changes. Plant Cell, Tissue and Organ Culture. 2020; 140(2): 245–257.
Bonga J.M., Von Aderkas P. In vitro culture of trees. Springer Science & Business Media. 1992.
Chandran H., Meena M., Barupal T., Sharma K. Plant tissue culture as a perpetual source for production of industrially important bioactive compounds. Biotechnology Reports. 2020; 26: e00450.
De Klerk G.J., Van der Krieken W., De Jong J.C. The formation of adventitious roots: new concepts, new possibilities. In Vitro Cellular and Developmental Biology – Plant. 1999; 35(3): 189–199.
Fattahi M., Nazeri V., Sefidkon F., Zamani Z.A. Investigation of the autecology of Danayi lemon balm (Dracocephalum kotschyi Boiss.) in Iran. Iranian Journal of Medicinal and Aromatic Plants Research. 2013; 29(6): 325–342.
George E.F., Hall M.A., De Klerk G.J. Plant propagation by tissue culture: Volume 1. The background. Springer. 2008.
Ghani A., Mohtashami S., Esmaeilpour M., Esfanani M. Investigation of the effect of different treatments on the stimulation of germination in the medicinal plant Zarrin (Dracocephalum kotschyi). Proceedings of the Ninth Congress of Horticultural Science, Ahvaz, Iran. 2015.
Hatami M., Samadi S., Khanizadeh S. Effect of different treatments on dormancy breaking and germination stimulation of seeds of Zarrin (Dracocephalum kotschyi Boiss). Iranian Journal of Rangeland and Desert Research. 2019; 26(4): 918–931.
Husen A. Clonal propagation of Dalbergia sissoo Roxb. through axillary shoot proliferation. Scientia Horticulturae. 2008; 115(3): 320–326.
Ikeuchi M., Sugimoto K., Iwase A. Plant callus: mechanisms of induction and repression. The Plant Cell. 2016; 28(5): 1009–1024.
Jahanian F., Ebrahimi S.A., Rahbar Roshandel N., Mahmoudian M. Xanthomicrol is the main cytotoxic component of Dracocephalum kotschyi and a potential anticancer agent. Phytochemistry. 2005; 66(13): 1581–1592.
Jain R., Sinha P., Gupta A. Influence of plant growth regulators on direct regeneration of Stevia rebaudiana and its genetic fidelity assessment through RAPD. Plant Cell Biotechnology and Molecular Biology. 2009; 10(3–4): 101–108.
Jonoubi M., Zamani Nasrabadi M. Indirect regeneration of the endangered medicinal plant Zarrin (Dracocephalum kotschyi Boiss). Developmental Biology. 2018; 10(1): 33–42.
Kumar A., Mishra P., Jha B. Efficient regeneration from hypocotyl explants of Jatropha curcas and evaluation for genetic fidelity using RAPD and ISSR markers. Industrial Crops and Products. 2010; 32(3): 491–498.
Lloyd G., McCown B., Amerson H. Commercially feasible micropropagation of mountain laurel (Kalmia latifolia) by use of shoot-tip culture. Combined Proceedings of the International Plant Propagators' Society. 1983; 30: 421–427.
Moradi K., Otroshy M. Trichomes and regeneration by direct organogenesis of medicinal plant Dracocephalum kotschyi L. using shoot tips (Lamiaceae). Journal of Crop Science and Biotechnology. 2012; 15(3): 251–257.
Otroshy M., Moradi K. Micropropagation of medicinal plant Dracocephalum kotschyi Boiss. via nodal cutting technique. Journal of Medicinal Plants Research. 2011; 5(25): 5967–5972.
Patel R.M., Shah R.R. Regeneration of plants from nodal and apical explants of Ocimum sanctum L. In Vitro Cellular and Developmental Biology – Plant. 2009; 45(4): 442–449.
Razavizadeh R., Adabavazheh F. In vitro application of chitosan effects on essential oil content and physiological characteristics of Dracocephalum kotschyi Boiss. Journal of Plant Process and Function. 2018; 8(31): 23–30.
Sharada M., Bhagyalakshmi N., Chandrasekharan S. In vitro clonal propagation of Withania somnifera through nodal explants. Phytomorphology. 2003; 53(2): 203–209.
Skoog F., Miller C.O. Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symposium of the Society for Experimental Biology. 1957; 11: 118–131.
Taghizadeh M., Nasibi F., Kalantari K.M., Benakashani F. Callogenesis optimization and cell suspension culture establishment of Dracocephalum polychaetum Bornm. and Dracocephalum kotschyi Boiss.: An in vitro approach for secondary metabolite production. South African Journal of Botany. 2020; 132: 79–86.
Thorpe T.A. History of plant tissue culture. Molecular Biotechnology. 2007; 37(2): 169–180
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سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب
