Investigation of growth and proliferation of GN15 rootstock using growth regulators and sugars in vitro | ||
| پژوهش در علوم باغبانی | ||
| Volume 4, Issue 2, September 2025, Pages 52-64 PDF (477.83 K) | ||
| Document Type: Research | ||
| DOI: 10.22092/rhsj.2026.370625.1132 | ||
| Author | ||
| Sahar Toopchizadeh* | ||
| Managing Director of Arda Textile Company, located in the Village of Technology and Innovation in Agriculture, Natural Resources and Food Industries of East Azerbaijan Province | ||
| Abstract | ||
| Rootstock is one of the main factors in determining the seedlings adaptation to environmental conditions and its resistance to biotic and abiotic stresses. In this study, the effect of plant growth regulators including cytokinin in two forms, BAP and KIN (at concentrations of 1 and 2 mg/L), auxin in the form of NAA (at concentrations of 0 and 0.1 mg/L), and gibberellic acid (at concentrations of 0, 0.5, and 1 mg/L), and the effect of sugars including sucrose, glucose, and sorbitol (each at concentrations of 15, 30, and 45 mg/L) were investigated in micropropagation of the almond peach hybrid GN15. After establishment and growth of the micro-samples, traits such as proliferation rate, number of branches, shoot longitudinal growth, number of leaves, and root longitudinal growth were measured. By comparing the mean data, the maximum proliferation rate was observed using BAP at a concentration of 2 mg/L along with GA at a concentration of 0.5 mg/L. The highest number of leaves was obtained in plants treated with 2 mg/L BAP along with 1 mg/L GA, in the absence of NAA hormone. For promoting the longitudinal growth of shoots and roots, nutritional treatment with sucrose (30 g/L) showed the highest efficiency. In general, the rate of proliferation and number of leaves are mainly affected by hormonal compounds (BAP or KIN with GA), while the longitudinal growth of shoots and roots is mainly increased by the use of sucrose. | ||
| Keywords | ||
| Auxin; Peach-almond hybrid; Sucrose; Cytokinin; Sorbitol and Invitro culture | ||
| References | ||
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