Investigation of the effects of different concentrations of thyme (Zataria mulltiora) extract on three species of edible mushrooms and their fungal pathogens | ||
| مهار زیستی در گیاهپزشکی | ||
| Articles in Press, Accepted Manuscript, Available Online from 14 February 2026 | ||
| Document Type: Short Article | ||
| DOI: 10.22092/bcpp.2026.371503.406 | ||
| Authors | ||
| zeinab zare rahmat abad1; Seyed Esmaeil Razavi* 2; Minoo Rastegar3 | ||
| 1Ph. D Graduated Student in Plant Pathology, Department of Plant Protection, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. | ||
| 2Associated Professor, Department of Plant Protection, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. | ||
| 3M. Sc. Center Laboratory, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. | ||
| Abstract | ||
| Today, many studies have been conducted on the antimicrobial properties of edible coatings. Many flavonoids and plant extracts found in plants have antimicrobial properties. Zataria multiflora extract plays a crucial role in inhibiting factors that cause decay and extending the shelf life of food. Various pests and diseases are the most important factors limiting the cultivation and breeding of edible mushrooms, and among them, fungal diseases are one of the most important and serious problems in the cultivation of edible mushrooms that affect their yield and quality. Therefore, the aim of this study is to investigate the effect of different concentrations of thyme extract on pathogens isolated from button mushrooms (Agaricus bisporus). Therefore, in order to identify fungal pathogens, sampling was carried out in the fall of 2021 from edible mushroom cultivation halls in the eastern counties of Golestan province and its suburbs. Also, in the present study, five different concentrations of thyme (82.5, 125, 187.5, 200, and 300 ppm) were tested in vitro on four pathogenic fungi isolated from button mushrooms, as well as two species of edible oyster mushrooms (Pleurotus eryngii and P. florida) and one species of edible button mushroom (Agaricus bisporus). Thyme was obtained from the Gorgan Essential Oil Pharmaceutical Company and then extracted using an ethanol/water solvent (75 parts alcohol and 25 parts water). In this study, the antimicrobial test was performed by creating wells in the culture medium, and 0.125 ml of thyme extract with the studied concentrations was added to the wells. In laboratory conditions, the colony diameter index (radial growth) of pathogenic and edible fungi and the percentage of inhibition of the growth of the desired fungi by thyme extracts were investigated. In this study, the results of pathogen identification showed that Mycogon perniciosa, Trichoderma harzianum and T. vierens, Cephalosporium costantini, and Alternaria alternata species were the pathogens of edible mushroom production halls in the eastern counties of Golestan province. Also, the results of pathogen inhibition using different concentrations of thyme extract showed that a concentration of 200 ppm of thyme extract caused a decrease of about 10, 9, and 27 percent in the colony growth of pathogenic fungi Trichoderma harzianum, Alternaria alternata, and Cephalosporium costantini, respectively. In this study, thyme extract acted selectively in inhibiting the growth and did not affect the mycelium growth of the edible mushrooms A. bisporus, P. florida, and P. eryngii. | ||
| Keywords | ||
| Ethanol extract; oyster mushroom; Mycogon perniciosa; inhibition percentage; Agaricus bisporus | ||
| References | ||
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Abbott, W.S. 1987. A mothod of computing the effectiveness of an insecticide 1925. Journal of the American Mosquito Control Association, 3: 302–303. Al–Balushi, Y.J.R., Al–Sadi, A.M., Al–Mahmooli, I.H., Al–Harrasi, M.M.A., Al–Sabahi, J.N., Al–Alawi, A.K.S. & Al–Farsi, K. 2022. Antifungal Activity of Shirazi Thyme (Zataria multiflora Boiss.) Essential Oil against Hypomyces perniciosus, a causal agent of wet bubble disease of Agaricus bisporus, Journal of Agricultural and Marine Sciences, 27(2): 59–65. https:/ /doi.org/10.53541/jams. Arnold, G. R. W. & Yurchenko, E. O. 2007. The first contribution on fungi from Belarus. Mycena, 7: 4–19. Domesch, K. H., Gams, W. & Anderson, T. H. 1980. Compendium of soil fungi. London: Academica, 21(1): 859–865. Durling, N.E., Catchpole, O.J., Grey, J.B., Webby, R.F., Mitchell, K.A., Foo, L. Y. & Perry, N. B. 2007. Extraction of phenolics and essential oil from dried sage () using ethanol–water Mixtures. Food chemistery, 101: 1417–24. Enyiukwu, D.N., Awurum, A.N., Ononuju, C.C. & Nwanerij, A. 2014. Significance of characterization of secondary metabolites from extracts of higer plants in plant disease management. International journal of Advance Agricaltural Research, 8–28. Gea, F. J., Navarro, M. J., Santos, M., Diánez, F. & Herraiz–Peñalver, D. 2019. Screening and evaluation of essentialoils from Mediterranean aromatic plants against the mushroom cobweb disease, Cladobotryum mycophilum. Agronomy, 9(10): 656–69. DOI: 10.3390/agronomy9100656. Ghuffar, S., Irshad, G., Naz, F., Khan, M.A., Ahmed, M.Z. & Gleason, M.L. 2022. Efficacy of plant essential oils against Alternaria alternate causing bunch rot of grapes in Pakistan. Journal of Animal and Plant Sciences, 32: 150–162. Glamoclija, J., Sokovic, M., Vukojevi, J., Milenkovic, I. & van Griensven, L. 2008. Morphological characteristics and mycelial compatibility of different Mycogone perniciosa isolates. Journal of Microscopy, 232: 489–492. Kotan, R., Cakir, A., Dadasoglu, F., Aydin, T., Cakmakci, R., Ozer, H., Kordali, S., Mete, E. & Dikbas, N. 2010. Antibacterial activities of essential oils and extracts of Turkish Achillea, Satureja and Thymus species against plant pathogenic bacteria. Journal of the Science of Food and Agriculture, 90: 145–160. Largeteau, M.L. & Savoie, J.M. 2010. Microbially induced diseases of Agaricus bisporus: Biochemical mechanisms and impact on commercial mushroom production. Applied Microbiology and Biotechnology, 86: 63–73. Mehrparvar, M., Mohammadi Goltapeh, E. & Safaei, N. 2016. Resistance of Iranian Lecanicillium fungicola to Benzimidazole and Ergosterol Demethylation Inhibiting Fungicides. Journal of Agricultural Science and Technology, 15: 389–396. Nateqi, M. & Mirghazanfari, S.M. 2018. Determination of total phenolic content, antioxidant activity and antifungal effects of Thymus vulgaris, Trachyspermum ammi and Trigonella foenum–graecum extracts on growth of Fusarium solani. Cellular and Molecular Biology, 39–46. Rifai, M.A. 1969. A revision of genus Trichoderma. Commonwolth Mycologycal institute, Pap. 116–156. Stevi´c, T., Beri´c, T., Savikin, K., Sokovi´c, M., Godzevac, D., Dimki´c, I. & Stankovi´c, S. 2014. Antifungal activity of selected essential oils against fungi isolated from medicinal plant. Industrial Crops and Products, 55: 116–122. | ||
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