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Molecular and morphological evidence for two Clonostachys and one Sesquicillium species new to Iran | ||
| Rostaniha | ||
| دوره 26، شماره 1 - شماره پیاپی 72، دی 2025، صفحه 60-76 اصل مقاله (1.27 M) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22092/bot.j.iran.2025.370380.1429 | ||
| نویسندگان | ||
| Maedeh Pourshirmohammadi1؛ Mohammad Javad Pourmoghaddam2؛ Salar Jamali* 3؛ Seyed Akbar Khodaparast* 4؛ Sedigheh Mousanejad3 | ||
| 1MSc Student in Plant Pathology, Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| 2Post Doc. Researcher, Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| 3Associate Prof., Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| 4Prof., Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| چکیده | ||
| To identify some fungal species associated with root–knot nematode (Meloidogyne species) or inhabiting the rhizosphere of Kiwifruit trees (Actinidia chinensis), infected roots and rhizospheric soil samples were collected from Kiwifruit orchards in Guilan Province (North of Iran). For fungal isolation from soil, one g of each soil sample was suspended in 100 mL of sterile distilled water. After making serial dilutions, 100 µL of each dilution was spread onto PDA-Rose Bengal agar supplemented with streptomycin. For isolation from nematodes, egg masses were surface-sterilized in 0.5% sodium hypochlorite, transferred to the same culture medium, and incubated at 25 °C in the dark for 10 days. The isolates were characterized using the morphological assessment and DNA sequence analyses of the internal transcribed spacer (ITS), translation elongation factor 1-α (tef1), and beta-tubulin (tub2) regions. Some isolates produced monomorphic or dimorphic conidiophores typical of members of the Clonostachys and Sesquicillium genera. Morphological and molecular data confirmed that, the isolates represented three species, including Clonostachys chloroleuca (from rhizospheric soli), C. rogersoniana (from a Meloidogyne sp. egg sac), and Sesquicillium aff. essexcoheniae (from rhizospheric soil). To the best of the authors’ knowledge, this is the first report of these species from Iran. | ||
| کلیدواژه‌ها | ||
| Biodiversity؛ Bionectriaceae؛ Kiwifruit؛ Meloidogyne؛ phylogeny | ||
| عنوان مقاله [English] | ||
| شواهد مولکولی و ریخت‌شناسی برای دو گونه Clonostachys و یک گونه Sesquicillium جدید در ایران | ||
| نویسندگان [English] | ||
| مائده پورشیرمحمدی1؛ محمدجواد پورمقدم2؛ سالار جمالی3؛ سیداکبر خداپرست4؛ صدیقه موسی‌نژاد3 | ||
| 1دانشجوی کارشناسی ارشد گروه گیاه‌پزشکی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران | ||
| 2محقق پسادکتری گروه گیاه‌پزشکی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران | ||
| 3دانشیار گروه گیاه‌پزشکی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران | ||
| 4استاد گروه گیاه‌پزشکی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران | ||
| چکیده [English] | ||
| به منظور شناسایی برخی گونه‌های قارچی مرتبط با گونه‌های Meloidogyne یا موجود در ریزوسفر درختان کیوی (Actinidia chinensis)، نمونه‌هایی از گونه‌های Meloidogyne و خاک ریزوسفر از باغ‌های کیوی در استان گیلان جمع‌آوری شد. برای جداسازی قارچ از خاک، یک گرم از هر نمونه خاک در ۱۰۰ میلی‌لیتر آب مقطر سترون معلق گردید. پس از تهیه رقت‌های متوالی، ۱۰۰ میکرولیتر از هر رقت روی محیط کشت PDA-Rose Bengal حاوی استرپتومایسین پخش شد. برای جداسازی از نماتدها، توده‌های تخم با محلول هیپوکلریت سدیم نیم درصد ضدعفونی سطحی شده، به محیط کشت مشابه منتقل و به مدت ۱۰ روز در دمای ۲۵ درجه سلسیوس در تاریکی نگهداری شدند. جدایه‌ها با استفاده از ارزیابی ریخت‌شناختی و تجزیه و تحلیل توالی DNA نواحی فاصله‌دهنده رونویسی‌شونده داخلی (ITS)، فاکتور طویل شدن ترجمه یک آلفا (tef1) و بتا-توبولین (tub2) شناسایی شدند. برخی از جدایه‌ها کنیدیوفورهای مونومورفیک یا دیمورفیک معمول اعضای جنس‌های Clonostachys و Sesquicillium تولید کردند. داده‌های ریخت‌شناختی و مولکولی تأیید کردند که جدایه‌ها متعلق به سه گونه: Clonostachys chloroleuca (از ریزوسفر)، C. rogersoniana (از کیسه تخم .Meloidogyne sp) و Sesquicillium aff. essexcoheniae (از ریزوسفر) هستند. براساس اطلاعات نگارندگان، این نخستین گزارش از گونه‌های مذکور از ایران است. | ||
| کلیدواژه‌ها [English] | ||
| تبارشناسی, تنوع زیستی, کیوی, نماتد مولد غده, Bionectriaceae | ||
| مراجع | ||
|
Abbasi, K. & Afzalinia, S. 2022. Identification of eight fungi isolated from potato golden cyst nematode from Iran. Plant Pathology Science 11(2): 22–31. Abdollahi Aghdam, S. & Fotouhifar, K.B. 2017. Identification of some endophytic fungi of cherry trees (Prunus avium) in Iran. Iranian Journal of Plant Protection Science 48(1): 43–57. Abreu, L.M., Moreira, G.M., Ferreira, D., Rodrigues-Filho, E. & Pfenning, L.H. 2014. Diversity of Clonostachys species assessed by molecular phylogenetics and MALDI-TOF mass spectrometry. Fungal Biology 118(12): 1004–1012. DOI: 10.1016/j.funbio.2014.10.001. Alves, A., Crous, P.W., Correia, A. & Phillips, A.J.L. 2008. Morphological and molecular data reveal cryptic speciation in Lasiodiplodia theobromae. Fungal Diversity 28: 1–13. DOI: 10.1002/yea.1554. Bao, D.F., Hyde, K.D., Maharachchikumbura, S.S.N., Perera, R.H., Thiyagaraja, V., Hongsanan, S., Wanasinghe, D.N., Shen, H.W., Tian, X.G., Yang, L.Q., Nalumpang, S. & Luo, Z.L. 2023. Taxonomy, phylogeny and evolution of freshwater Hypocreomycetidae (Sordariomycetes). Fungal Diversity 121(1): 1–94. DOI: 10.1007/s13225-023-00521-8. Carling, D.E. & Sumner, D.R. 1992. Rhizoctonia. Pp. 157–165. In: Methods for research on soilborne phytopathogenic fungi (Singleton, L.L., Mihail, J.D. & Rush, C.M.; eds). Carbone, I. & Kohn, L.M. 1999. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91: 553–556. DOI: 10.1080/00275514.1999.12061051. Ebrahimi, L. & Fotouhifar, K. 2016. Identification of some fungi accompanying the scab symptoms in Iran. Mycologia Iranica 3(1): 25–37. DOI: 10.22043/mi.2017.48716.1077. Edler, D., Klein, J., Antonelli, A. & Silvestro, D. 2021. RaxmlGUI 2.0: A graphical interface and toolkit for phylogenetic analyses using RAxML. Methods in Ecology and Evolution 12: 373–377. DOI: 10.1111/2041- 210X.13512. Ghahremani, F., Khodaparast, S.A., Pourmoghaddam, M.J. & Mousanejad, S. 2025. Diaporthe species associated with Blackberry and Blueberry from Guilan Province, Iran. Mycologia Iranica 12(1): 43–57. DOI: 10.22092/mi.2025.368317.1306. Han, P., Zhang, X., Xu, D., Zhang, B., Lai, D. & Zhou, L. 2020. Metabolites from Clonostachys Fungi and Their Biological Activities. Journal of Fungi 6: 229. DOI: 10.3390/jof6040229. He, S., Thiyagaraja, V., Bhunjun, C.S., Chomnunti, P., Dissanayake, L.S., Jayawardena, R.S., ... et al. 2025. Morphology and multi-gene phylogeny reveal three new species of Clonostachys and two combinations of Sesquicillium (Bionectriaceae, Hypocreales) from Xizang, China. MycoKeys 115: 43–66. DOI: 10.3897/mycokeys.115.139757. Hou, L.W., Giraldo, A., Groenewald, J.Z., Rämä, T., Summerbell, R.C., Huang, G.Z., Cai, L. & Crous, P.W. 2023. Redisposition of acremonium-like fungi in Hypocreales. Studies in Mycology 105: 23–203. DOI: 10.3114/sim.2023.105.02. Hyde, K.D., Noorabadi, M.T., Thiyagaraja, V., Mq, H., Johnston, PR., Wijesinghe, S.N., Armand, A., ... et al. 2024. The 2024 Outline of Fungi and fungus-like taxa. Mycosphere 15(1): 5146–6239. DOI: 10.5943/mycosphere/15/1/25. Katoh, K., Rozewicki, J. & Yamada, K.D. 2019. MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20(4): 1160–1166. DOI: 10.1093/bib/bbx108. Kapeua-Ndacnou, M., de Abreu, L.M., de Macedo, D.M., da Nóbrega, T.F., Pereira, C.M., Evans, H.C. & Barreto, R.W. 2023. Assessing the biocontrol potential of Clonostachys species isolated as endophytes from Coffea species and as mycoparasites of Hemileia rusts of coffee in Africa. Journal of Fungi 9(2): 248. DOI: 10.3390/jof9020248. Kumar, S., Stecher, G. & Tamura, K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33 1870–1874. DOI: 10.1093/molbev/msw054.Epub. Lechat, C. & Fournier, J. 2018. Clonostachys spinulosispora (Hypocreales, Bionectriaceae), a new species on palm from French Guiana. Ascomycete.org 10: 127–130. Lechat, C., Fournier, J., Chaduli, D., Lesage-Meessen, L. & Favel, A. 2019. Clonostachys saulensis (Bionectriaceae, Hypocreales), a new species from French Guiana. Ascomycete.org 11: 65–68. Leslie, J.F. & Summerell, B.A. 2006. The Fusarium Laboratory Manual. Blackwell Publishing. Ames, IA. Maddison, W.P. & Maddison, D.R. 2015. Mesquite: a modular system for evolutionary analysis. Version 3.10. http://mesquiteproject.org. Mahmoudi, H., Amini, A., Mirzaee, M.R., Sadeghi, H. & Tavakkoli, G.R. 2018. Clonostachys rosea, a new and promising entomopathogenic fungus infecting pupa of jujube fruit fly, Carpomya vesuviana. Mycologia Iranica 5(1): 43–49. DOI: 10.22043/mi.2019.118753. Moreira, G.M., Abreu, L.M., Carvalho, V.G., Schroers, H.J. & Pfenning, L.H. 2016. Multilocus phylogeny of Clonostachys subgenus Bionectria from Brazil and description of Clonostachys chloroleuca sp. nov. Mycological Progress 15: 1031–1039. DOI: 10.1007/s11557-016-1224-6. Onkar, D. & James, B.S. 1985. Basic Plant Pathology Methods. Boca Raton, FL: CRC Press. 306 pp. O’Donnell, K., Kistler H.C., Cigelnik E. & Ploetz R.C. 1998. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proceedings of the National Academy of Sciences of the United States of America 95(5): 2044–2049. DOI: 10.1073/pnas.95.5.2044. Preedanon, S., Suetrong, S., Srihom, C., Somrithipol, S., Kobmoo, N., Saengkaewsuk, S., Srikitikulchai, P., Klaysuban, A., Nuankaew, S., Chuaseeharonnachai, C., Chainuwong, B., Muangsong, C., Zhang, Z.F., Cai, L. & Boonyuen, N. 2023. Eight novel cave fungi in Thailand’s Satun Geopark. Fungal Systematics and Evolution 12: 1–30. DOI: 10.3114/fuse.2023.12.01. Rossman, A.Y., McKemy, J.M., Pardo-Schultheiss, R.A. & Schroers, H.J. 2001. Molecular studies of the Bionectriaceae using large subunit rDNA sequences. Mycologia 93: 100–110. DOI: 10.1080/00275514.2001.12061283. Rossman, A.Y, Seifert, K.A., Samuels, G.J., Minnis, A.M., Schroers, H.J., Lombard, L., Crous, P.W., Põldmaa, K., Cannon, P.F., Summerbell, R.C., Geiser, D.M., Zhuang, W.Y., Hirooka, Y., Herrera, C., Salgado-Salazar, C. & Chaverri, P. 2013. Genera of Bionectriaceae, Hypocreaceae and Nectriaceae (Hypocreales) proposed for acceptance and rejection. IMA Fungus 4: 41–51. DOI: 10.5598/imafungus.2013.04.01.05. Schroers, H.J. 2001. A monograph of Bionectria ascomycota, Hypocreales, Bionectriaceae and its Clonostachys anamorphs. Studies in Mycology 46: 1–214. Singh, S. & Mathur, N. 2010. In vitro studies of antagonistic fungi against the root-knot nematode, Meloidogyne incognita. Biocontrol Science and Technology 20(3): 275–282. DOI: 10.1080/09583150903484318. Tan, Y.P. & Shivas, R.G. 2023. Nomenclatural novelties. Index of Australian Fungi 5: 1–12. Torcato, C., Gonalves, M.F.M, Rodríguez-Gálvez, E. & Alves, A. 2020. Clonostachys viticola sp. nov., a novel species isolated from Vitis vinifera. International Journal of Systematic and Evolutionary Microbiology 70: 4321–4328. DOI: 10.1099/ijsem.0.004286. Vu, D., Groenewald, M., De Vries, M., Gehrmann, T., Stielow, B., Eberhardt, U., Al-Hatmi, A., Groenewald, J.Z., Cardinali, G., Houbraken, J., Boekhout, T., Crous, P.W., Robert, V. & Verkley, G.J.M. 2019. Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom fungi and reveals thresholds for fungal species and higher taxon delimitation. Studies in Mycology 92: 135–154. DOI: 10.1016/j.simyco.2018.05.001. White, T.J., Bruns, T., Lee, S. & Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal genes for phylogenetics. Pp: 315–322. In: PCR protocols: a guide to methods and applications (Gelfand, M., Sninsky, J.I. & White, T.J.; eds). Academic Press, New York, DOI: 10.1016/B978-0-12-372180-8.50042-1. Wang, Y., Tang, D.X., Luo, R., Wang, Y.B., Thanarut, C., Dao, V.M. & Yu, H. 2023. Phylogeny and systematics of the genus Clonostachys. Frontiers in Microbiology 14: 1117753. DOI: 10.3389/fmicb.2023.1117753. Yao, H., Liu, K., Peng, L., Huang, T., Shi, J., Sun, B. & Zou, J. 2024. Clonostachys rosea, a Pathogen of Brown Rot in Gastrodia elata in China. Biology 13(9): 730. DOI: 10.3390/biology13090730. Zeng, Z.Q. & Zhuang, W.Y. 2022. Three New Species of Clonostachys (Hypocreales, Ascomycota) from China. Journal of Fungi 8(10): 1027. DOI: 10.3390/jof8101027. Zhao, L., Groenewald, J.Z., Hernández-Restrepo, M., Schroers, H.J. & Crous, P.W. 2023. Revising Clonostachys and allied genera in Bionectriaceae. Studies in Mycology 105: 204–65. DOI: 10.3114/sim.2023.105.03. Zhao, L., Groenewald, J.Z., Hou, L.W., Summerbell, R.C. & Crous, P.W. 2025. Bionectriaceae: a poorly known family of hypocrealean fungi with major commercial potential. Studies in Mycology 111: 115–198. DOI: 10.3114/sim.2025.111.04. Zhang, Y.J., Zhang, S., Liu, X., Wen, H.A. & Wang, M. 2010. A simple method of genomic DNA extraction suitable for analysis of bulk fungal strains. Letters in Applied Microbiology 51(1): 114–118. DOI: 10.1111/j.1472-765X.2010.02867.x. Zhang, Z.Y., Li, X., Chen, W.H., Liang, J.D. & Han, Y.F. 2023. Culturable fungi from urban soils in China II, with the description of 18 novel species in Ascomycota (Dothideomycetes, Eurotiomycetes, Leotiomycetes and Sordariomycetes). MycoKeys 98: 167–220. DOI: 10.3897/mycokeys.98.102816. Zheng, C.J., Kim, C.J., Bae, K.S., Kim, Y.H. & Kim, W.G. 2006. Bionectins A-C, epidithiodioxopiperazines with anti-MRSA activity, from Bionectra byssicola F120. Journal of Natural Products 69(12): 1816–1819. DOI: 10.1021/np060348t. | ||
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