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Re-evaluation of the specificity of Pyricularia oryzae diagnostic primers and introduction of a cost-effective, species-level screening method for Pyricularia isolates using an ISSR marker | ||
| Mycologia Iranica | ||
| دوره 12، شماره 2، اسفند 2025، صفحه 31-45 اصل مقاله (1.74 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22092/mi.2025.370318.1321 | ||
| نویسندگان | ||
| Esmaeil Hashemlou1؛ Didier Tharreau2؛ Mohammad Javan-Nikkhah* 1؛ Khalil-Berdi Fotouhifar1؛ Amir Mirzadi Gohari1؛ Hossein Saremi1 | ||
| 1Department of Plant Protection, Faculty of Agriculture, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran | ||
| 2PHIM Plant Health Institute, Univ Montpellier, INRAE, CIRAD, Institute Agro, IRD, Montpellier, France | ||
| چکیده | ||
| Pyricularia oryzae is a devastating pathogen affecting rice and a wide range of cereal crops. Accurate and specific detection of this species is essential for population genetics, epidemiological studies, and the enforcement of quarantine measures. In this study, the specificity of several P. oryzae-specific diagnostic primer pairs, including pfh2a/pfh2b, Pot2a-L2/Pot2a-R2, mif23_01F/mif23_01R, MIF-forward/MIF-reverse, MHP1F/MHP1R, and PoM-1F/PoM-1R were re-evaluated using in silico and PCR assays. In addition, the efficacy of an ISSR marker was assessed as a cost-effective, species-level screening method for a large set of Pyricularia isolates. The results of in silico analysis revealed that PoM-1F/PoM-1R primer pair was not species-specific, while four other primers showed probable specificity for P. oryzae detection. However, PCR assays revealed that none of the tested P. oryzae-specific primers could reliably distinguish this species from other formally accepted species within the genus Pyricularia. These results highlight the urgent need to develop novel molecular markers that can unambiguously differentiate P. oryzae from related Pyricularia species. Such markers would significantly improve the diagnosis and surveillance of blast diseases. Meanwhile, the ISSR-PCR banding patterns produced from 36 strains representing seven formally accepted Pyricularia species were reproducible and congruent with phylogenetic species recognition. Therefore, in studies with large numbers of isolates, where cost-effective grouping is required prior to multi-gene sequencing, this marker represents a highly beneficial tool for selecting representative isolates corresponding to distinct species. | ||
| کلیدواژهها | ||
| Cereal Blast؛ Magnaporthe oryzae؛ Molecular detection؛ Pyriculariaceae؛ Species-specific primers | ||
| مراجع | ||
|
Ahmadpour A, Ghosta Y, Alavi Z, Alavi F, Poursafar A, Rampelotto PH. 2025a. Diversity of Alternaria section Nimbya in Iran, with the description of eight new species. Journal of Fungi. 11(3): 225. https://doi.org/10.3390/jof11030225.
Ahmadpour A, Ghosta Y, Poursafar A. 2021. Novel species of Alternaria section Nimbya from Iran as revealed by morphological and molecular data. Mycologia. 113(5): 1073-1088. https://doi.org/10.1080/00275514.2021.1923299.
Ahmadpour, A., Ghosta, Y., Alavi, F., Alavi, Z., Hashemlou, E., Kumla, J., Karunarathna, S.C. and Suwannarach, N. 2025b. Additions to Macgarvieomyces in Iran: morphological and phylogenetic analyses reveal six new species. Journal of Fungi. 11(7): 489. https://doi.org/10.3390/jof11070489.
Akbarzadeh S, Alizadeh A, Ahmadpour A, Shirzad A. 2023. Genetic diversity of Colletotrichum fructicola isolates using ISSR molecular marker and efficiency of this technique in differentiating this species from several Colletotrichum species. Journal of Applied Research in Plant Protection. 11(4): 43-61. https://doi.org/10.22034/arpp.2022.15600.
Alves A, Phillips AJ, Henriques I, Correia A. 2007. Rapid differentiation of species of Botryosphaeriaceae by PCR fingerprinting. Research in Microbiology. 158(2): 112-121. https://doi.org/10.1016/j.resmic.2006.10.003.
Ascari JP, Cazón LI, Rahnama M, Lamour K, Fernandes JM, ... et al. 2024. Pyricularia are mostly host-specialized with limited reciprocal cross-infection between wheat and endemic grasses in Minas Gerais, Brazil. Phytopathology. 114(1): 226-240. https://doi.org/10.1094/PHYTO-01-23-0024-R.
Baudin M, Le Naour‐Vernet M, Gladieux P, Tharreau D, Lebrun MH, ... et al. 2024. Pyricularia oryzae: Lab star and field scourge. Molecular Plant Pathology. 25(4): e13449. https://doi.org/10.1111/mpp.13449.
Bussaban B, Lumyong S, Lumyong P, Hyde KD, McKenzie EH. 2003. Three new species of Pyricularia are isolated as zingiberaceous endophytes from Thailand. Mycologia. 95(3): 519-524. https://doi.org/10.1080/15572536.2004.11833098.
Bussaban B, Lumyong S, Lumyong P, Seelanan T, Park DC, McKenzie EH, Hyde KD. 2005. Molecular and morphological characterization of Pyricularia and allied genera. Mycologia. 97(5): 1002-1011. https://doi.org/10.1080/15572536.2006.11832750.
Castroagudín VL, Moreira SI, Pereira DA, Moreira SS, Brunner PC, ... et al. 2016. Pyricularia graminis-tritici, a new Pyricularia species causing wheat blast. Persoonia. 37(1): 199-216. https://doi.org/10.3767/003158516X692149.
Chadha S. 2019. Molecular detection of Magnaporthe oryzae from rice seeds. In: Magnaporthe oryzae: Methods and Protocols (Jacob J. ed): (187-197). Springer, USA. https://doi.org/10.1007/978-1-0716-1613-0_15.
Chadha S, Gopalakrishna T. 2006. Detection of Magnaporthe grisea in infested rice seeds using polymerase chain reaction. Journal of Applied Microbiology, 100(5): 1147-1153. https://doi.org/10.1111/j.1365-2672.2006.02920.x.
Couch BC, Fudal I, Lebrun MH, Tharreau D, Valent B, ... et al. 2005. Origins of host-specific populations of the blast pathogen Magnaporthe oryzae in crop domestication with subsequent expansion of pandemic clones on rice and weeds of rice. Genetics. 170(2): 613-630. https://doi.org/10.1534/genetics.105.041780.
Crous PW, Wingfield MJ, Burgess TI, Hardy G, Crane C, … et al. 2016. Fungal Planet description sheets: 469-557. Persoonia. 37: 218-403. https://doi.org/10.3767/003158516X694499.
Ellis MB. 1971. Dematiaceous hyphomycetes. Commonwealth Mycological Institute, England.
Ellis MB. 1976. More dematiaceous hyphomycetes. C.A.B International Mycological Institute, England.
Ganesan S, Singh HS, Petikam S, Biswal D. 2017. Pathological status of Pyricularia angulata causing blast and pitting disease of banana in Eastern India. The Plant Pathology Journal. 33(1): 9-20. https://doi.org/10.5423/PPJ.OA.08.2016.0162.
Ghaderi O, Abdollahzadeh J. 2025. Biodiversity of Aspergillus species in some dried fruits and the traditional food Terkhêna, with description of Aspergillus mahabadiensis sp. nov. from Mahabad, western Iran. Mycologia Iranica. 12(1): 21-42. https://doi.org/10.22092/mi.2025.368273.1305.
Gladieux P, Condon B, Ravel S, Soanes D, Maciel JL, ... et al. 2018. Gene flow between divergent cereal-and grass-specific lineages of the rice blast fungus Magnaporthe oryzae. mBio. 9(1): e01219-17. https://doi.org/10.1128/mbio.01219-17.
Harmon PF, Dunkle LD, Latin R. 2003. A rapid PCR-based method for the detection of Magnaporthe oryzae from infected perennial ryegrass. Plant Disease. 87(9): 1072-1076. https://doi.org/10.1094/PDIS.2003.87.9.1072.
Hirata K, Kusaba M, Chuma I, Osue J, Nakayashiki H, Mayama S, Tosa Y. 2007. Speciation in Pyricularia inferred from multilocus phylogenetic analysis. Mycological Research.111(7):799-808. https://doi.org/10.1016/j.mycres.2007.05.014.
Huang CM, Liao DJ, Wu HS, Shen WC, Chung CL. 2016. Cyclone‐based spore trapping, quantitative real‐time polymerase chain reaction and high-resolution melting analysis for monitoring airborne inoculum of Magnaporthe oryzae. Annals of Applied Biology. 69(1): 75-90. https://doi.org/10.1111/aab.12282.
Ioos R, Tharreau D. 2025. Pyricularia oryzae lineage Triticum causing wheat blast disease: An emerging threat to the EPPO region. EPPO Bulletin, 00:1–8. https://doi.org/10.1111/epp.13094.
Jayawardena RS, Hyde KD, de Farias AR, Bhunjun CS, Ferdinandez HS, … et al. 2021. What is a species in fungal plant pathogens?. Fungal Diversity. 109(1): 239-266. https://doi.org/10.1007/s13225-021-00484-8.
Katoh K, Rozewicki J, Yamada KD. 2019. MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics. 20(4): 1160-1166. https://doi.org/10.1093/bib/bbx108.
Klaubauf S, Tharreau D, Fournier E, Groenewald JZ, Crous PW, ... et al. 2014. Resolving the polyphyletic nature of Pyricularia (Pyriculariaceae). Studies in Mycology. 79(1): 85-120. https://doi.org/10.1016/j.simyco.2014.09.004.
Kumar P, Akhtar J, Meena BR, Tiwari R, Chalam VC. 2025. Development of multiplex PCR protocols for simultaneous detection of Alternaria padwickii, Bipolaris oryzae, Pyricularia oryzae and Ustilaginoidea virens in rice seed. Physiological and Molecular Plant Pathology. 9: 102786. https://doi.org/10.1016/j.pmpp.2025.102786.
Lassagne A, Brun S, Malagnac F, Adreit H, Milazzo J, … et al. 2022. Male fertility in Pyricularia oryzae: Microconidia are spermatia. Environmental Microbiology. 24(12): 6365-6375. https://doi.org/10.1111/1462-2920.16226.
Luo J, Zhang N. 2022. Diseases and Management. In: The Rice Blast Fungus and Allied Species: A Monograph of the Fungal Order Magnaporthales (Luo J, Zhang N. eds): 29-35. The American Phytopathological Society, USA. https://doi.org/10.1094/9780890546833.002.
Maddison WP, Maddison DR. 2023. Mesquite: a modular system for evolutionary analysis. Version 3.81. Available from: http://www.mesquiteproject.org.
Male MF, Tan YP, Vawdrey LL, Shivas RG. 2011. Elucidation of the taxonomy and pathological status of Pyricularia associated with banana blast in Australia. Australasian Plant Disease Notes. 6(1): 22-25. https://doi.org/10.1007/s13314-011-0008-8.
Marin-Felix Y, Groenewald JZ, Cai LM, Chen Q, Marincowitz S, ... et al. 2017. Genera of phytopathogenic fungi: GOPHY 1. Studies in Mycology. 86: 99-216. https://doi.org/10.1016/j.simyco.2017.04.002.
Marin-Felix Y, Hernández-Restrepo M, Wingfield MJ, Akulov A, Carnegie AJ, ... et al. 2019. Genera of phytopathogenic fungi: GOPHY 2. Studies in Mycology. 92: 47-133. https://doi.org/10.1016/j.simyco.2018.04.002.
Miller MA, Pfeiffer W, Schwartz T. 2010. Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: 2010 gateway computing environments workshop (GCE) 2010 Nov 14 :1-8 https://doi.org/10.1109/GCE.2010.5676129.
Nylander JAA. 2004 MrModeltest 2.3. Computer program and documentation distributed by the author. Evolutionary Biology Centre, Uppsala University.
Odeph M, Luasi WW, Kavoo A, Mweu C, Ngugi M, ... et al. 2020. Occurrence, distribution and severity of finger millet blast caused by Magnaporthe oryzae in Kenya. African Journal of Plant Science. 14(4): 139-49. https://doi.org/10.5897/AJPS2020.1970.
Oliveira M, Azevedo L. 2022. Molecular markers: An overview of data published for fungi over the last ten years. Journal of Fungi. 8(8): 803. https://doi.org/10.3390/jof8080803.
Ou SH. 1985. Rice Disease. 2nd ed, CAB International, UK.
Pappas AC, Paplomatas EJ. 1998. Pyricularia leaf spot: a new disease of ornamental plants of the family Marantaceae. Plant Disease. 82(5):465-469. https://doi.org/10.1094/PDIS.1998.82.5.465.
Patro TS, Georgia KE, Praveen B, Palanna KB, Anuradha N. 2021. The blast: a major malady in nutricereals in Southeast Asia. In: Blast Disease of Cereal Crops: Evolution and Adaptation in Context of Climate Change (Nayaka S, Hosahatti R, Prakash G, Satyavathi C, Sharma R. eds): 135-141. Springer, Switzerland. https://doi.org/10.1007/978-3-030-60585-8_9.
Pieck ML, Ruck A, Farman ML, Peterson GL, Stack JP, ... et al. 2017. Genomics-based marker discovery and diagnostic assay development for wheat blast. Plant Disease. 101(1): 103-109. https://doi.org/10.1094/PDIS-04-16-0500-RE.
Rambaut A. 2019. FigTree, a graphical viewer of phylogenetic trees. Available from: http://tree.bio.ed.ac.uk/software/figtree.
Ronquist F, Huelsenbeck JP. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 19(12): 1572-1574. https://doi.org/10.1093/bioinformatics/btg180.
Stamatakis A.2014. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics. 30(9): 1312-1313. https://doi.org/10.1093/bioinformatics/btu033.
Standish JR, Góngora-Castillo E, Bowman MJ, Childs KL, Tian M, ... et al. 2022 Development, validation, and utility of species-specific diagnostic markers for detection of Peronospora belbahrii. Phytopathology 112(8): 1667-1775. https://doi.org/10.1094/PHYTO-09-21-0393-R.
Su’udi M, Kim J, Park JM, Bae SC, Kim D, ... et al. 2013. Quantification of rice blast disease progressions through TaqMan real-time PCR. Molecular Biotechnology. 55(1): 43-48. https://doi.org/10.1007/s12033-012-9632-6.
Swofford DL. 2003. PAUP^* phylogenetic analysis using parsimony (^* and other methods). Version 4. Available from: https://phylosolutions.com/paup-test/
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution. 30(12): 2725-2729. https://doi.org/10.1093/molbev/mst197.
Thierry M, Chatet A, Fournier E, Tharreau D, Ioos R. 2020a. A PCR, qPCR, and LAMP toolkit for the detection of the wheat blast pathogen in seeds. Plants. 9(2): 277. https://doi.org/10.3390/plants9020277.
Thierry M, Gladieux P, Fournier E, Tharreau D, Ioos R. 2020b. A genomic approach to develop a new qPCR test enabling detection of the Pyricularia oryzae lineage causing wheat blast. Plant Disease. 104(1): 60-70. https://doi.org/10.1094/PDIS-04-19-0685-RE.
Tosa Y, Inoue Y, Trinh TV, Chuma I. 2016. Genetic and molecular analyses of the incompatibility between Lolium isolates of Pyricularia oryzae and wheat. Physiological and Molecular Plant Pathology. 95: 84-86. https://doi.org/10.1016/j.pmpp.2016.01.007.
Valent B, Farman M, Tosa Y, Begerow D, Fournier E, ... et al. 2019. Pyricularia graminis‐tritici is not the correct species name for the wheat blast fungus: response to Ceresini et al. (MPP 20:2). Molecular Plant Pathology. 20(2): 173-179. https://doi.org/10.1111/mpp.12778.
Venbrux M, Crauwels S, Rediers H. 2023. Current and emerging trends in techniques for plant pathogen detection. Frontiers in Plant Science. 14: 1120968. https://doi.org/10.3389/fpls.2023.1120968.
Villari C, Mahaffee WF, Mitchell TK, Pedley KF, Pieck ML, ... et al. 2017. Early detection of airborne inoculum of Magnaporthe oryzae in turfgrass fields using a quantitative LAMP assay. Plant Disease. 101(1): 170-177. https://doi.org/10.1094/PDIS-06-16-0834-RE.
Wang J, Wang Q, Anchieta A, Chen R, Dai X, ... et al. 2025. Species-specific primers derived from comparative genomics for PCR detection of Verticillium species. Plant Disease. https://doi.org/10.1094/PDIS-10-24-2227-RE.
Zhang N, Zhao S, Shen Q. 2011. A six-gene phylogeny reveals the evolution of mode of infection in the rice blast fungus and allied species. Mycologia. 103(6): 1267-1276. https://doi.org/10.3852/11-022.
Zhou S, Smith DR, Stanosz GR. 2001. Differentiation of Botryosphaeria species and related anamorphic fungi using Inter Simple or Short Sequence Repeat (ISSR) fingerprinting. Mycological Research. 105(8): 919-926. https://doi.org/10.1016/S0953-7562(08)61947-4. | ||
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