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Evaluation of a newly established rapid and selective graphene-based kit for diagnosis of hydatidosis by using nano ELISA and nano-immunochromatography | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 13 مرداد 1404 اصل مقاله (432.63 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2025.367899.3445 | ||
| نویسندگان | ||
| Manal Ahmed1؛ shadia Hassan1؛ Ibrahim Shalash2؛ Rabab Zalat2؛ Marwa Aboueldahab3؛ Ahmed Hamdy Nigm* 4 | ||
| 1Department of Zoology, Faculty of Science, Ain Shams University, 11566, Abbassia, Cairo, Egypt | ||
| 2Department of Parasitology, Theodor Bilharz Research Institute, Cairo, Egypt | ||
| 3Department of Zoology, Faculty of science, Ain Shams University, Abbasia, Cairo, Egypt | ||
| 4Zoology department, Faculty of Science, Ain Shams University, Abbasia- Ghalifa Mamoon street - cairo -Egypt | ||
| چکیده | ||
| Hydatid disease (HD) or hydatidosis, is a major public health problem that is spreading all over the world. The diagnosis of hydatid disease is considered to be challenging; however, the serological diagnosis provides an early protocol, and confirmation of the existence of the hydatid cyst microscopically is still a must. Enzyme-linked immunosorbent assay (ELISA) is one of the best diagnostic methods. Sandwich ELISA, nano-sandwich ELISA and immunochromatographic test (ICT) were conducted utilizing purified hydatid antigen for diagnosis of human hydatidosis. The analyzed sera comprised samples from patients infected with hydatidosis (n = 50) and sera of patients infected with helminthic parasites (20, 20 and 10 with Schistosoma mansoni, Fasciola hepatica, and Ancylostoma duodenale, respectively), and 50 healthy negative controls. The findings indicated that the sandwich ELISA detected positive results in 39 out of 50 hydatid-infected samples, yielding an assay sensitivity of 78%. Seven out of fifty parasite-infected groups yielded positive results, resulting in 93% specificity. In the nano-sandwich ELISA, 42 of 50 hydatid-infected samples yielded positive results, which produced an assay sensitivity of 84%. 7 out of 50 other parasite-infected groups had positive results giving 93% specificity. Using immunochromatographic test; Thirty-six out of fifty hydatid-infected samples yielded positive results, which gave an assay sensitivity of 72%. 16 out of 50 other parasite-infected groups had positive results, giving 80% specificity. Diagnostic specificity reached 93%, 93% and 80% by using sandwich ELISA, nano-sandwich ELISA and ICT techniques respectively. The use of nano-sandwich ELISA is one of the best methods for diagnosis of human hydatidosis | ||
| کلیدواژه‌ها | ||
| Echinococcosis؛ Schistosoma؛ Fasciola؛ Ancylostoma؛ Diagnostic Kit | ||
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