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Evaluation of a Newly Established Rapid and Selective Graphene-based Kit for Diagnosis of Hydatidosis by Using Nano ELISA and Nano-immunochromatography | ||
| Archives of Razi Institute | ||
| مقاله 11، دوره 81، شماره 1، فروردین و اردیبهشت 2026، صفحه 89-98 اصل مقاله (1.06 M) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.66224/ARI.81.1.3445 | ||
| نویسندگان | ||
| Manal Ahmed1؛ Shadia H. Mohamed1؛ Ibrahim R. Shalash2؛ Rabab S. M. Zalat2؛ Marwa M. Aboueldahab1؛ Ahmed M. Nigm* 1 | ||
| 1Department of Zoology, Faculty of Science, Ain Shams University, Cairo, Egypt. | ||
| 2Department of Parasitology, Theodor Bilharz Research Institute, Cairo, Egypt. | ||
| چکیده | ||
| Introduction: Hydatid disease (HD) or hydatidosis is a major public health problem that is spreading all over the world. The diagnosis of hydatid disease is considered to be challenging; however, serological diagnosis provides an early diagnostic approach, and confirmation of the existence of the hydatid cyst microscopically is still necessary. Enzyme-linked immunosorbent assay (ELISA) is one of the best diagnostic methods. Materials & Methods: Sandwich ELISA, nano-sandwich ELISA, and the immunochromatographic test (ICT) were conducted utilizing purified hydatid antigen for the diagnosis of human hydatidosis. The analyzed sera comprised samples from patients infected with hydatidosis (n=50), patients infected with other helminthic parasites (20, 20, and 10 with Schistosoma mansoni, Fasciola hepatica, and Ancylostoma duodenale, respectively), and 50 healthy negative control subjects. Results: The findings indicated that the sandwich ELISA detected positive results in 39 out of 50 hydatid-infected samples, yielding an assay sensitivity of 78%. Seven out of 50 samples from parasite-infected groups yielded positive results, resulting in 93% specificity. In the nano-sandwich ELISA, 42 of 50 hydatid-infected samples yielded positive results, which produced an assay sensitivity of 84%. seven out of 50 samples from other parasite-infected groups had positive results, giving 93% specificity. Using the ICT, 36 out of 50 hydatid-infected samples yielded positive results, which gave an assay sensitivity of 72%. sixteen out of 50 from other parasite-infected groups had positive results, giving 80% specificity. Diagnostic specificity reached 93%, 93%, and 80% by using sandwich ELISA, nano-sandwich ELISA, and ICT techniques, respectively. Conclusion: The use of nano-sandwich ELISA is one of the best methods for the diagnosis of human hydatidosis. | ||
| کلیدواژه‌ها | ||
| Ancylostoma؛ Diagnostic Kit؛ Echinococcosis؛ Fasciola؛ Schistosoma | ||
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