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بررسی بیان ژن های خانواده تیروزیناز (TYR، TYRP1 و DCT) و ژنMATP در بافت پوست گوسفندان لری بختیاری | ||
| علوم دامی | ||
| دوره 38، شماره 148، آذر 1404، صفحه 3-14 اصل مقاله (1.79 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2024.365765.2390 | ||
| نویسندگان | ||
| محسن رحمانی1؛ مصطفی محقق دولت آبادی* 2؛ جواد حبیبی زاد3 | ||
| 1فارغ التحصیل کارشناسی ارشد ژنتیک و اصلاح دام، گروه علوم دامی، دانشکده کشاورزی، دانشگاه یاسوج | ||
| 2دانشیار گروه علوم دامی، گروه علوم دامی، دانشکده کشاورزی، دانشگاه یاسوج | ||
| 3استادیار گروه علوم دامی، گروه علوم دامی، دانشکده کشاورزی، دانشگاه یاسوج | ||
| چکیده | ||
| به منظور بررسی تاثیر بیان ژن های خانواده تیروزیناز(TYR، TYRP1 و DCT) و ژن MATP بر روی ایجاد لکه های پوستی در گوسفند نژاد لری بختیاری، از مجموع تعداد 14 رأس گوسفند با رنگ پوشش سفید که دارای لکه‌های تیره روی پوشش خود بودند، نمونه پوست از هر دو بخش سفید و تیره تهیه و RNA کل استخراج شد. همچنین، پس از تعیین کیفیت و کمیت RNA استخراج شده، cDNA برای هر نمونه سنتز گردید. جهت تکثیر قطعه‌هایی از ژن‌های هدف و مرجع، آغازگر‌های لازم بر اساس توالی‌های mRNA موجود در GenBank و نرم افزار Primer3plus طراحی شدند. تجزیه و تحلیل داده‌های بیان ژن نشان داد که بیان ژن‌های DCT و MATP در بافت لکه‌دار پوست نسبت به بافت سفید پوست تغییر معنی‌داری نداشت . در بین ژن‌های هدف، ژن DCT با میانگین چرخه آستانه 78/35 دارای میزان بیان کمتری نسبت به ژن MATP با میانگین چرخه آستانه 68/31 در نمونه‌های پوست گوسفند نژاد لری‌بختیاری بودند. بیان ژن‌های TYR و TYRP1 در هیچ یک از نمونه‌ها از هر دو بخش پوست مشاهده نشد. بر اساس نتایج این پژوهش، سطح بیان ژن‌های DCT، TYR، TYRP1 و MATP نمی‌تواند نقش موثری در تنظیم رنگ پوشش و ایجاد لکه‌های سیاه در بافت پوست گوسفندان لری‌بختیاری داشته باشد و به نظر می‌رسد ژن‌ها یا عوامل دیگری باعث ایجاد لکه سیاه در این نژاد بوده که شناسایی آنها نیاز به مطالعات بیشتری دارد. | ||
| کلیدواژه‌ها | ||
| بیان ژن؛ پوست؛ رنگ پوشش؛ گوسفند | ||
| عنوان مقاله [English] | ||
| Expression analysis of tyrosinase family genes (TYR, TYRP1 and DCT) and MATP gene in the skin tissue of Lori Bakhtiari sheep | ||
| نویسندگان [English] | ||
| mohsen rahmani1؛ mustafa muhaghegh dolatabady2؛ javad hibibizad3 | ||
| 1Department of Animal Sciences faculty of Agriculture Yasouj University yasouj iran | ||
| 2Animal Science Department, Faculty of Agriculture Science, University of Yasouj, Yasouj, Iran | ||
| 3Animal Science Department, Faculty of Agriculture Science, University of Yasouj, Yasouj, Iran | ||
| چکیده [English] | ||
| The effect of mRNA levels in tyrosinase family genes (TYR, TYRP1 and DCT) and MATP gene on the development of skin spots were investigated in Lori Bakhtiari sheep. For this porpose, the skin samples were collected from both white and dark parts of 14 white-haired Lori-Bakhtiari sheep with black spots and total RNA was extracted. In addition, after determining the quality and quantity of extracted RNA, cDNA was synthesized for each sample. To amplify fragments of the target and reference genes, the necessary primers were designed based on the mRNA sequences available in GenBank and Primer3plus software. The result showed mRNA levels of DCT and MATP genes were not significantly different in spotted skin tissue compared to white skin tissue. compared to the white part of the skin tissue. Among the target genes, the DCT gene with an average threshold cycle (Ct) of 35.78 had a lower expression level than the MATP gene with an average Ct of 31.68 in skin samples of Lori-Bakhtiari sheep. The expression of TYR and TYRP1 genes were not detected in any of the samples from both parts of the skin. The results of this study showed that the expression level of DCT, TYR, TYRP1 and MATP genes can not play an effective role in regulating the coat color and creating black spots in the skin tissue of Lori Bakhtiari sheep. Therefore, it appears that genes or other factors cause black spots in Lori-Bakhtiari sheep, and their identification requires further study. | ||
| کلیدواژه‌ها [English] | ||
| gene expression, skin, coat color, sheep | ||
| مراجع | ||
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