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In Silico genomic fingerprints of the pathogenic cyanobacterial strains isolated from Alma-Gol Wetland (Golestan Province, Iran), using highly repeated sequences | ||
| Rostaniha | ||
| دوره 26، شماره 1 - شماره پیاپی 72، دی 2025، صفحه 27-48 اصل مقاله (2.2 M) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22092/bot.j.iran.2025.369416.1420 | ||
| نویسندگان | ||
| Marzieh Samadi1؛ Hassan Beiranvand1؛ Morteza Mohajeri َAmiri2؛ Bahareh Nowruzi* 3 | ||
| 1MSc Student, Department of Biology, SR.C., Islamic Azad University, Tehran, Iran | ||
| 2Assistant Prof., Department of Biology, SR.C., Islamic Azad University, Tehran, Iran | ||
| 3Associate Prof., Department of Biology, SR.C., Islamic Azad University, Tehran, Iran | ||
| چکیده | ||
| Alma-Gol Wetland (Golestan Province, Iran) is a permanent habitat for the cultivation of many fish and aquatic organisms and is a popular destination for many travelers during the warm seasons of the year. The discovery of several dead fish carcasses on the water surface indicated the presence of cyanotoxins in the said area. Therefore, the present study, is aimed to screen potentially toxic cyanobacterial strains in this wetland by amplifying mcyG, mcyD, and mcyE toxic genes, as well as structural genes including 16S rRNA and ITS. It was also employed the rep-PCR technique to amplify highly repetitive genes HIP, STRR, and ERIC. The results obtained from the dendrogram based on the amplification of palindromic primers showed that, strains Nostoc sp. 9, Aliinostoc sp. 1, and Desmonostoc sp. 10 clustered together, while strains Calothrix sp. 7 and Neowestiellopsis sp. 2 mostly formed separate branches. However, only the strain Neowestiellopsis sp. 2 contained the toxic gene mcyD. Furthermore, comparative analysis of the ITS region length revealed that, both the length and the structure of the D1-D1 and BOX B helices differed from those of other strains. This study is among the first to demonstrate that fingerprinting techniques can provide new insights into different cyanobacterial strains. | ||
| کلیدواژهها | ||
| Dendrogram؛ molecular phylogeny؛ palindromic primers؛ phylogenetic tree؛ toxic cyanobacteria | ||
| عنوان مقاله [English] | ||
| مطالعه انگشتنگاری ژنومی درونرایانهای سویههای سیانوباکتریایی بیماریزا جداشده از تالاب آلماگل (استان گلستان) با استفاده از توالیهای به شدت تکراری | ||
| نویسندگان [English] | ||
| مرضیه صمدی1؛ حسن بیرانوند1؛ مرتضی مهاجریامیری2؛ بهاره نوروزی3 | ||
| 1دانشجوی کارشناسی ارشد بخش زیستشناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 2استادیار بخش زیستشناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 3دانشیار بخش زیستشناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| چکیده [English] | ||
| تالاب آلماگل (استان گلستان)، محل دایمی پرورش بسیاری از ماهیها و موجودات آبزی، محل بازدید عمومی بسیاری از گردشگران در فصول گرم سال است. شناسایی اجساد مرده چندین ماهی روی سطح آب نشان از حضور سیانوباکتریهای بیماریزا (سیانوتوکسینها) در این منطقه بود. به همین دلیل در تحقیق حاضر، با تکثیر ژنهای بیماریزای mcyG، mcyD و mcyE و همچنین ژنهای ساختاری از جمله 16S rRNA و ITS به همراه استفاده از روش rep-PCR برای تکثیر ژنهای به شدت تکراری HIP، STRR و ERIC، به غربالگری سویه بالقوه سیانوباکتریهای بیماریزای این تالاب پرداخته شد. نتایج به دست آمده از دندروگرام حاصل از تکثیر پرایمرهای پالیندرومیک نشان داد سویههای Nostoc sp. 9، Aliinostoc sp. 1 و Desmonostoc sp. 10 در کلاد مشترک و سویههای Calothrix sp. 7 و Neowestiellopsis sp. 2 در بیشتر موارد، انشعابات جداگانهای را تشکیل دادند. با این حال، تنها سویه Neowestiellopsis sp. 2 حاوی ژن بیماریزای mcyD بود. همچنین، نتایج حاصل از آنالیز مقایسه طول مناطق ITS نشان داد که طول منطقه و همچنین ساختار مارپیچهای D1-D1 و BOX B با سایر سویهها متفاوت بود. مطالعه حاضر جزو نخستین تحقیقات انجام شده است که نشان داد میتوان با استفاده از روش انگشتنگاری، اطلاعات جدیدی را در زمینه سویههای مختلف سیانوباکتری ارایه نمود. | ||
| کلیدواژهها [English] | ||
| پرایمرهای پالیندرومیک, درخت فیلوژنتیک, دندروگرام, سیانوباکتریهای سمی, فیلوژنی مولکولی | ||
| مراجع | ||
|
Akoijam, C. & Singh, A.K. 2011. Molecular typing and distribution of filamentous heterocystous cyanobacteria isolated from two distinctly located regions in North-Eastern India. World Journal of Microbiology and Biotechnology 27(3): 2187–2194. DOI: 10.1007/s11274-011-0684-8. Baptista, M., Cunha, J.T. & Domingues, L. 2021. DNA-based approaches for dairy products authentication: A review and perspectives. Trends in Food Science & Technology 109(2): 386–397. DOI: 10.1016/j.tifs.2021.01.043. Becerra-Absalón, I., Rodarte, B., Osorio, K., Alba-Lois, L., Segal-Kischinevzky, C. & Montejano, G. 2013. A new species of Brasilonema (Scytonemataceae, Cyanoprokaryota) from Tolantongo, Hidalgo, Central Mexico. Fottea 13(5): 25–38. DOI: 10.5507/fot.2013.003. Bittner, M., Štern, A., Smutná, M., Hilscherová, K. & Žegura, B. 2021. Cytotoxic and genotoxic effects of cyanobacterial and algal extracts-Microcystin and retinoic acid content. Toxins 13(1): 107–121. DOI: 10.3390/toxins13020107. Cai, F., Peng, X. & Li, R. 2020. Violetonostoc minutum gen. et sp. nov. (Nostocales, Cyanobacteria) from a rocky substrate in China. Algae 35(2): 1–15. DOI:10.4490/algae.2020.35.3.4. Chonudomkul, D., Yongmanitchai, W., Theeragool, G., Kawachi, M., Kasai, F., Kaya, K. & Watanabe, M.M. 2004. Morphology, genetic diversity, temperature tolerance and toxicity of Cylindrospermopsis raciborskii (Nostocales, Cyanobacteria) strains from Thailand and Japan. FEMS Microbiology Ecology 48(11): 345–355. DOI: 10.1016/j.femsec.2004.02.014. De Bruijn, F.J. 1992. Use of repetitive (repetitive extragenic palindromic and enterobacterial repetitive intergeneric consensus) sequences and the polymerase chain reaction to fingerprint the genomes of Rhizobium meliloti isolates and other soil bacteria. Applied and Environmental Microbiology 58(1): 2180–2187. DOI: 10.1128/aem.58.7.2180-2187.1992. Desikachary, T.V. 1959. Cyanophyta, New Delhi. Indian Council of Agricultural Research 12(4): 1431–1500. DOI: 10.1007/s10811-012-9793-5. Fewer, D.P., Rouhiainen, L., Jokela, J., Wahlsten, M., Laakso, K., Wang, H. & Sivonen, K. 2007. Recurrent adenylation domain replacement in the microcystin synthetase gene cluster. BMC Evolutionary Biology 7(4): 1–11. DOI: 10.1186/1471-2148-7-183. Guajardo-Leiva, S., Pedrós-Alió, C., Salgado, O., Pinto, F. & Díez, B. 2018. Active crossfire between Cyanobacteria and Cyanophages in phototrophic mat communities within hot springs. Frontiers in Microbiology 9(1): 20–39. DOI: 10.3389/fmicb.2018.02039. Hulton, C., Higgins, C. & Sharp, P. 1991. ERIC sequences: a novel family of repetitive elements in the genomes of Escherichia coli, Salmonella typhimurium and other enterobacteria. Molecular Microbiology 5(1): 825–834. DOI: 10.1111/j.1365-2958.1991.tb00755.x. Iteman, I., Rippka, R., Tandeau de Marsac, N. & Herdman, M. 2000. Comparison of conserved structural and regulatory domains within divergent 16S rRNA–23S rRNA spacer sequences of cyanobacteria. Microbiology 146(3): 1275–1286. DOI: 10.1099/00221287-146-6-1275. Komárek, J. 2006. Cyanobacterial taxonomy: current problems and prospects for the integration of traditional and molecular approaches. Algae 21(2): 349–375. DOI: 10.4490/algae.2006.21.4.349. Komárek, J., Sant’Anna, C.L., Bohunicka, M., Mareš, J., Hentschke, G.S., Rigonato, J. & Fiore, M.F. 2013. Phenotype diversity and phylogeny of selected Scytonema species (Cyanoprokaryota) from SE Brazil. Fottea 13(3): 173–200. DOI: 10.5507/fot.2013.015. Kotai, J. 1972. Instruction of preparations of modified nutrient medium Z8 for algae. Norw. Institute of Water Research 4(3): 32–39. Lepère, C., Wilmotte, A. & Meyer, B. 2000. Molecular diversity of Microcystis strains (Cyanophyceae, Chroococcales) based on 16S rDNA sequences. Systematics and Geography of Plants 4(4): 275–283. DOI: 10.2307/3668646. Mutoti, M., Gumbo, J. & Jideani, A.I.O. 2022. Occurrence of cyanobacteria in water used for food production: A review. Physics and Chemistry of the Earth, Parts A/B/C 125(2): 103–121. DOI: 10.1016/j.pce.2021.103101. Neilan, B., Saker, M., Fastner, J., Törökné, A. & Burns, B. 2003. Phylogeography of the invasive cyanobacterium Cylindrospermopsis raciborskii. Molecular Ecology 12(1): 133–140. DOI: 10.1046/j.1365-294X.2003.01709.x. Neilan, B.A., Jacobs, D., Therese, D.D., Blackall, L.L., Hawkins, P.R., Cox, P.T. & Goodman, A.E. 1997. rRNA sequences and evolutionary relationships among toxic and nontoxic cyanobacteria of the genus Microcystis. International Journal of Systematic and Evolutionary Microbiology 47(4): 693–697. DOI: 10.1099/00207713-47-3-693. Nowruzi, B., Becerra-Absalón, I. & Metcalf, J.S. 2023. A novel microcystin-producing cyanobacterial species from the genus Desmonostoc, D. alborizicum sp. nov., isolated from a water supply system of Iran. Current Microbiology 80(1): 49–53. DOI: 10.1007/s00284-022-03144-5. Nowruzi, B. & Beiranvand, H. 2024. In vitro and in vivo study of the antifungal activity of extracellular products of cyanobacterium Neowestiellopsis persica strain A1387 against Fusarium wilt disease of cucumber. Revista Argentina de Microbiología 57(3): 182–197. DOI: 10.1016/j.ram.2024.10.011. Nowruzi, B., Beiranvand, H., Aghdam, F.M. & Barandak, R. 2024. The effect of plasma activated water on antimicrobial activity of silver nanoparticles biosynthesized by cyanobacterium Alborzia kermanshahica. BMC Biotechnology 24(2): 75–93. DOI: 10.1186/s12896-024-00905-x. Nowruzi, B., Blanco, S. & Nejadsattari, T.J.I.J.O.A. 2018. Chemical and Molecular Evidences for the Poisoning of a Duck by Anatoxin-a, Nodularin and Cryptophycin at the Coast of Lake Shoormast (Mazandaran Province, Iran). International Nowruzi, B., Bouaïcha, N., Metcalf, J.S., Porzani, S.J. & Konur, O. 2021. Plant-cyanobacteria interactions: Beneficial and harmful effects of cyanobacterial bioactive compounds on soil-plant systems and subsequent risk to animal and human health. Phytochemistry 192(11): 112–159. DOI: 10.1016/j.phytochem.2021.112959. Nowruzi, B. & Fahimi, H. 2022. Fingerprinting and molecular phylogeny of some heterocystous cyanobacteria using 16S rRNA, ITS regions and highly iterated palindromes as molecular markers. Rostaniha 23(2): 79–104. DOI: 10.22092/BOTANY.2022.358594.1306. Nowruzi, B. & Hutarova, L. 2023. Structural and functional genes, and highly repetitive sequences commonly used in the phylogeny and species concept of the phylum Cyanobacteria. Cryptogamie, Algologie 44(15): 59–84. DOI: 10.5252/cryptogamie-algologie2023v44a3. Nowruzi, B., Khavari-Nejad, R.-A., Sivonen, K., Kazemi, B., Najafi, F. & Nejadsattari, T. 2012. Identification and toxigenic potential of a Nostoc sp. Algae 27(12): 303–313. DOI: 10.4490/algae.2012.27.4.303. Nowruzi, B., Khavari-Nejad, R.A., Sivonen, K., Kazemi, B., Najafi, F. & Nejadsattari, T.J.P.I.B.S. 2013. Identification and toxigenic potential of a cyanobacterial strain (Stigomena sp.). Algae 3(2): 79–85. DOI: 10.4490/algae.2012.27.4.303. Nowruzi, B. & Lorenzi, A.S. 2024. Morphological and molecular characterization of Goleter sp. (Nostocales, Nostocaceae) isolated from freshwater in Iran. Cryptogamie, Algologie 45(1): 39–51. DOI: 10.5252/cryptogamie-algologie2024v45a4. Nowruzi, B. & Shalygin, S. 2021. Multiple phylogenies reveal a true taxonomic position of Dulcicalothrix alborzica sp. nov. (Nostocales, Cyanobacteria). Fottea 21(4): 235–246. DOI: 10.5507/fot.2021.008. Nowruzi, B. & Soares, F. 2021. Alborzia kermanshahica gen. nov., sp. nov. (Chroococcales, Cyanobacteria), isolated from paddy fields in Iran. International Journal of Systematic and Evolutionary Microbiology 71(4): 14–26. DOI: 10.1099/ijsem.0.004828. Nowruzi, B. & Zakerfirouzabad, M. 2024. Antifungal activity of Neowestiellopsis persica against Rhizoctonia solani in root and crown of Faba bean cultivated under modified BG-110 medium composition. The Microbe 4(11): 51–71. DOI: 10.1016/j.microb.2024.100112. Orcutt, K., Rasmussen, U., Webb, E.A., Waterbury, J.B., Gundersen, K. & Bergman, B. 2002. Characterization of Trichodesmium spp. by genetic techniques. Applied and Environmental Microbiology 68(13): 2236–2245. DOI: 10.1128/AEM.68.5.2236-2245.2002. Rantala, A., Rajaniemi-Wacklin, P., Lyra, C., Lepistö, L., Rintala, J., Mankiewicz-Boczek, J. & Sivonen, K. 2006. Detection of microcystin-producing cyanobacteria in Finnish lakes with genus-specific microcystin synthetase gene E (mcyE) PCR and associations with environmental factors. Applied and Environmental Microbiology 72(4): 6101–6110. DOI: 10.1128/AEM.01058-06. Rasmussen, U. & Svenning, M.M. 1998. Fingerprinting of cyanobacteria based on PCR with primers derived from short and long tandemly repeated repetitive sequences. Applied and Environmental Microbiology 64(3): 265–272. DOI: 10.1128/AEM.64.1.265-272.1998. Rouhiainen, L., Sivonen, K., Buikema, W.J. & Haselkorn, R. 1995. Characterization of toxin-producing cyanobacteria by using an oligonucleotide probe containing a tandemly repeated heptamer. Journal of Bacteriology 177(15): 6021–6026. DOI: 10.1128/jb.177.20.6021-6026.1995. Sánchez-Baracaldo, P., Bianchini, G., Wilson, J.D. & Knoll, A.H. 2022. Cyanobacteria and biogeochemical cycles through earth history. Trends in Microbiology 30(17): 143–157. DOI: 10.1016/j.tim.2021.05.008. Selvakumar, G. & Gopalaswamy, G. 2008. PCR based fingerprinting of Westiellopsis cultures with short tandemly repeated repetitive (STRR) and highly iterated palindrome (HIP) sequences. Biologia 63(2): 283–288. DOI: 10.2478/s11756-008-0065-4. Shokraei, R., Fahimi, H., Blanco, S. & Nowruzi, B. 2019. Genomic fingerprinting using highly repetitive sequences to differentiate close cyanobacterial strains. Microbial Bioactivity 2(13): 068–075. DOI: 10.25163/microbbioacts.21015A2624310119. Singh, P., Kaushik, M.S., Srivastava, M. & Mishra, A.K. 2014. Phylogenetic analysis of heterocystous cyanobacteria (Subsections IV and V) using highly iterated palindromes as molecular markers. Physiology and Molecular Biology of Plants 20(5): 331–342. DOI: 10.1007/s12298-014-0244-4. Smith, J., Parry, J., Day, J. & Smith, R. 1998. A PCR technique based on the Hipl interspersed repetitive sequence distinguishes cyanobacterial species and strains. Microbiology 144(34): 2791–2801. DOI: 10.1099/00221287-144-10-2791. Tavakoli, Y., Mohammadipanah, F., Te, S.H., You, L. & Gin, K.Y.-H. 2021. Biodiversity, phylogeny and toxin production profile of cyanobacterial strains isolated from lake Latyan in Iran. Harmful Algae 106(10): 102–154. DOI: 10.1016/j.hal.2021.102054. Valerio, E., Chambel, L., Paulino, S., Faria, N., Pereira, P. & Tenreiro, R. 2009. Molecular identification, typing and traceability of cyanobacteria from freshwater reservoirs. Microbiology 155(4): 642–656. DOI: 10.1099/mic.0.022848-0. Wilson, A.E., Sarnelle, O., Neilan, B.A., Salmon, T.P., Gehringer, M.M. & Hay, M.E. 2005. Genetic variation of the bloom-forming cyanobacterium Microcystis aeruginosa within and among lakes: implications for harmful algal blooms. Applied and Environmental Microbiology 71(1): 6126–6133. DOI: 10.1128/aem.71.10.6126-6133.2005. Wilson, K.M., Schembri, M.A., Baker, P.D. & Saint, C.P. 2000. Molecular characterization of the toxic cyanobacterium Cylindrospermopsis raciborskii and design of a species-specific PCR. Applied and Environmental Microbiology 66(12): 332–338. DOI: 10.1128/AEM.66.1.332-338.2000. Zheng, W., Song, T., Bao, X., Bergman, B. & Rasmussen, U. 2002. High cyanobacterial diversity in coralloid roots of cycads revealed by PCR fingerprinting. FEMS Microbiology Ecology 40(6): 215–222. DOI: 10.1111/j.1574-6941.2002.tb00954.x. | ||
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